Date published: 2025-9-8
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EEA1 Antibody (281.7) - Western Blotting - Image 353163
EEA1 抗体 (281. 7): sc-53939. JAR (A), Jurkat (B), HeLa (C), HEK293 (D) 和 A-431 (E) 全细胞裂解液中 EEA1 表达的 Western 印迹分析.
EEA1 抗体 (281. 7): sc-53939. JAR (A), Jurkat (B), HeLa (C), HEK293 (D) 和 A-431 (E) 全细胞裂解液中 EEA1 表达的 Western 印迹分析.

EEA1 抗体 (281.7): sc-53939

3.3(4)
寫評論提問

说明书
  • EEA1抗体(281.7)是小鼠单克隆IgG1 κ, 在12篇文献中引用，规格为200 µg/ml
  • 与重组蛋白结合，该重组蛋白与EEA1的82-1411的氨基酸对应，来源于human
  • 推荐用于 mouse, rat 和 human 来源的EEA1 WB, IP, IF 和 IHC(P)检测
  • 看 EEA1 (G-4): sc-137130，可查看有标记的 EEA1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 EEA1 抗体 (281.7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EEA1 抗体 (281.7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

早期内体是细胞质中的隔室，在接收和分拣内吞蛋白质以供囊泡运输中发挥作用。EEA1（早期内体抗原1）是一种外围膜蛋白，在早期内体与转铁蛋白受体和Rab5共定位。EEA1包含一个钙调蛋白结合IQ基序和富含半胱氨酸的指状基序，这对于其特定位于早期内体至关重要。EEA1与几种涉及膜转运的酵母蛋白质具有序列同源性，包括Vps27、Fab1和Vac1。有证据表明，EEA1可能在介导3'-磷酸肌醇对膜转运的调节作用中扮演重要角色。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

EEA1 抗体 (281.7)

sc-53939
200 µg/ml
RMB2377.00

EEA1 (281.7): m-IgG Fc BP-HRP 套装

sc-537017
200 µg Ab; 10 µg BP
RMB2662.00

EEA1 (281.7): m-IgGκ BP-HRP 套装

sc-534198
200 µg Ab; 40 µg BP
RMB2662.00

EEA1 (281.7): m-IgG1 BP-HRP 套装

sc-544958
200 µg Ab; 20 µg BP
RMB2662.00

I am using EEA1 (281.7): sc-53939 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not too sure.Did not work in HEK 293 for Immunofluorescence....
Date published: 2016-10-27
Rated 4 out of 5 by from Positive signal in WB in APositive signal in WB in A-431 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by from Produced wonderful immunoperoxidase cytoplasmicProduced wonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-01-26
Rated 4 out of 5 by from Good publishable data in WB in human HeLaGood publishable data in WB in human HeLa, HCT116, and U87MG cells. -SCBT Publication Review
Date published: 2015-01-03
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EEA1 抗体 (281.7) is rated 3.3 out of 5 by 4.
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