Date published: 2025-9-8
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EEA1 Antibody (E-8) - Western Blotting - Image 374829
EEA1 抗体 (E-8): sc-365652. NIH/3T3 (A), RAW 264. 7 (B), 3611-RF (C), HeLa (D), Jurkat (E) 和 NAMALWA (F) 全细胞裂解液中 EEA1 表达的 Western 印迹分析.
EEA1 抗体 (E-8): sc-365652. NIH/3T3 (A), RAW 264. 7 (B), 3611-RF (C), HeLa (D), Jurkat (E) 和 NAMALWA (F) 全细胞裂解液中 EEA1 表达的 Western 印迹分析.

EEA1 抗体 (E-8): sc-365652

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寫評論提問

说明书
  • EEA1抗体(E-8)是小鼠单克隆IgG1 κ, 在24篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的EEA1的N-terminus的氨基酸2-27之间
  • 抗-EEA1 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human 和monkey 来源的 EEA1; 也和以下物种反应，包括： porcine
  • 抗EEA1抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EEA1 (E-8): sc-365652的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是EEA1 Antibody (E-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与EEA1 Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

EEA1抗体（E-8）是一种IgG1 κ小鼠单克隆EEA1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的EEA1。EEA1抗体（E-8）既可以非偶联的抗EEA1抗体形式提供，也可以多种偶联形式的抗EEA1抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。早期内体是细胞质中的隔室，在接收和分拣内吞蛋白质以供囊泡运输中发挥作用。EEA1（早期内体抗原1）是一种外围膜蛋白，在早期内体与转铁蛋白受体和Rab5共定位。EEA1包含一个钙调蛋白结合IQ基序和富含半胱氨酸的指状基序，这对于其特定位于早期内体至关重要。EEA1与几种涉及膜转运的酵母蛋白质具有序列同源性，包括Vps27、Fab1和Vac1。有证据表明，EEA1可能在介导3'-磷酸肌醇对膜转运的调节作用中扮演重要角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EEA1 抗体 (E-8) 参考文献:

  1. 小 GTP 酶 Rab22 与 EEA1 相互作用，控制着内体膜贩运。  |  Kauppi, M., et al. 2002. J Cell Sci. 115: 899-911. PMID: 11870209
  2. 诱导p38丝裂原活化蛋白激酶可减少早期内体自身抗原1（EEA1）向吞噬泡膜的募集。  |  Fratti, RA., et al. 2003. J Biol Chem. 278: 46961-7. PMID: 12963735
  3. 液泡遗传和液泡蛋白分选所需的基因 VAC1 的分子特征。  |  Weisman, LS. and Wickner, W. 1992. J Biol Chem. 267: 618-23. PMID: 1730622
  4. 感染巴西副球孢子菌的巨噬细胞中早期内体抗原1（EEA1）减少。  |  Voltan, AR., et al. 2013. Med Mycol. 51: 759-64. PMID: 23566224
  5. 内体成熟过程中，由稳定素受体内化的反义寡核苷酸的内体逃逸受Rab5C和EEA1调控。  |  Miller, CM., et al. 2018. Nucleic Acid Ther. 28: 86-96. PMID: 29437530
  6. VPS27 通过酿酒酵母的前液泡区控制液泡和内细胞交通。  |  Piper, RC., et al. 1995. J Cell Biol. 131: 603-17. PMID: 7593183
  7. 新型 PI(4)P 5- 激酶同源物 Fab1p 对酵母正常液泡功能和形态至关重要  |  Yamamoto, A., et al. 1995. Mol Biol Cell. 6: 525-39. PMID: 7663021
  8. EEA1，一种早期内膜相关蛋白。EEA1 是一种保守的α-螺旋外周膜蛋白，两侧有半胱氨酸 '指'，并含有钙调蛋白结合 IQ 基序。  |  Mu, FT., et al. 1995. J Biol Chem. 270: 13503-11. PMID: 7768953
  9. 自身抗原 EEA1 的内体定位是由锌结合 FYVE 手指介导的。  |  Stenmark, H., et al. 1996. J Biol Chem. 271: 24048-54. PMID: 8798641
  10. Rab4 和 Rab7 界定了不同的, 不重叠的内膜区。  |  Bottger, G., et al. 1996. J Biol Chem. 271: 29191-7. PMID: 8910576
  11. 微管参与了巴佛洛霉素 A1 诱导的管化和早期内体的 Rab5 依赖性空泡化。  |  D'Arrigo, A., et al. 1997. Eur J Cell Biol. 72: 95-103. PMID: 9157016
  12. 鉴定受磷脂酰肌醇 3- 激酶调控的早期内体蛋白  |  Patki, V., et al. 1997. Proc Natl Acad Sci U S A. 94: 7326-30. PMID: 9207090

订购信息

产品名称产品编号规格价格数量收藏夹

EEA1 抗体 (E-8)

sc-365652
200 µg/ml
RMB2377.00

EEA1 (E-8): m-IgG Fc BP-HRP 套装

sc-529426
200 µg Ab; 10 µg BP
RMB2662.00

EEA1 (E-8): m-IgGκ BP-HRP 套装

sc-522313
200 µg Ab, 40 µg BP
RMB2662.00

EEA1 (E-8): m-IgG1 BP-HRP 套装

sc-543511
200 µg Ab; 20 µg BP
RMB2662.00

EEA1 抗体 (E-8) AC

sc-365652 AC
500 µg/ml, 25% agarose
RMB3129.00

EEA1 抗体 (E-8) HRP

sc-365652 HRP
200 µg/ml
RMB2377.00

EEA1 抗体 (E-8) FITC

sc-365652 FITC
200 µg/ml
RMB2482.00

EEA1 抗体 (E-8) PE

sc-365652 PE
200 µg/ml
RMB2580.00

EEA1 抗体 (E-8) Alexa Fluor® 488

sc-365652 AF488
200 µg/ml
RMB2685.00

EEA1 抗体 (E-8) Alexa Fluor® 546

sc-365652 AF546
200 µg/ml
RMB2685.00

EEA1 抗体 (E-8) Alexa Fluor® 594

sc-365652 AF594
200 µg/ml
RMB2685.00

EEA1 抗体 (E-8) Alexa Fluor® 647

sc-365652 AF647
200 µg/ml
RMB2685.00

EEA1 抗体 (E-8) Alexa Fluor® 680

sc-365652 AF680
200 µg/ml
RMB2685.00

EEA1 抗体 (E-8) Alexa Fluor® 790

sc-365652 AF790
200 µg/ml
RMB2685.00

EEA1 (E-8) 中和勝肽

sc-365652 P
100 µg/0.5 ml
RMB511.00

How is the G-4 different from the E-8 antibody?

Asked by: racsumbr
Thank you for your question. These two mouse monoclonal antibodies are raised against different EEA1 antigens and have distinct epitopes. EEA1 Antibody (G-4): sc-137130 is raised against a recombinant protein antigen corresponding to amino acids 1-300 of EEA1 of human origin. And EEA1 Antibody (E-8): sc-365652 is raised against a peptide mapping between amino acids 2-27 of EEA1 of human origin.
Answered by: Technical Support
Date published: 2018-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-20
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Rated 1 out of 5 by from Low signal in VeroE6 cells (IF)I used this Ab to stain endoscopes in VeroE6 cells by IF), but I got almost no signal (1:100-1:200, methanol fixation). That may depend on the cell line, however.
Date published: 2021-05-04
Rated 5 out of 5 by from WBHEK293T細胞のWBを行い、1:1000希釈ではっきりした単一のバンドを予想された高さに認めました。
Date published: 2018-09-22
Rated 5 out of 5 by from 免疫染色固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用して強い染色性が得られました。
Date published: 2018-03-22
Rated 5 out of 5 by from EEA1EEA1 works well in immunocytochemistry at dilution1:600 in cultured methanol-fixed epidermal keratinocytes
Date published: 2018-01-24
Rated 5 out of 5 by from GOOD Ab!i tried with hippocampal culture, very good Ab for WB!
Date published: 2017-11-22
Rated 5 out of 5 by from Great productGreat product for both western blot and IHC staining
Date published: 2017-02-05
Rated 5 out of 5 by from Popular antibody!Great Western blots, excellent IHC results. Reliable and consistent, this is a great choice for EEA1 detection.
Date published: 2016-12-30
Rated 5 out of 5 by from Robust signal in WB in ARobust signal in WB in A-431, NIH/3T3, Jurkat, C6 and COS whole cell lysates. -SCBT QC
Date published: 2015-04-24
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EEA1 抗体 (E-8) is rated 4.6 out of 5 by 10.
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