Date published: 2025-9-8

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EB1 抗体 (F-7): sc-374474

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说明书
  • EB1抗体(F-7)是小鼠单克隆IgA κ, 在4篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的EB1的C-terminus的氨基酸239-268之间
  • 推荐用于 mouse, rat 和 human 来源的EB1 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 看 EB1 (1A11/4): sc-47704,可查看有标记的 EB1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是EB1 Antibody (F-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与EB1 Antibody (F-7) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

EB1(末端结合蛋白1),也称为微管相关蛋白RP/EB家族成员1(MAPRE1)或APC结合蛋白EB1,可能会影响结直肠癌的肿瘤发生和正常细胞的增殖控制。EB1属于中间/早期基因家族,参与T细胞受体(TRC)下游的信号转导级联。结直肠癌是由正常结肠上皮向腺瘤性息肉的病理性转化引起的,这种息肉可变成侵袭性癌症。APC(腺瘤性结肠息肉病)是一种抑癌基因,其突变是结直肠癌发生过程中的最早事件之一。大多数突变导致APC的羧基末端丢失。已显示EB1与APC的羧基末端区域结合,这表明EB1参与APC对结肠癌的抑制。EB1的过度表达可能通过影响APC功能和激活β-连环蛋白/TCF途径在人类食管鳞状细胞癌的发生中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EB1 抗体 (F-7) 参考文献:

  1. 经典MAPs调节神经元中的EB1/3蛋白。  |  Sayas, CL. and Avila, J. 2014. Bioarchitecture. 4: 1-5. PMID: 24452057
  2. 非新月体微管通过 CAMSAP2-EB1 交叉对话调控自噬体转运  |  Wei, J., et al. 2017. FEBS Lett. 591: 2379-2393. PMID: 28726242
  3. EB1结合巨细胞蛋白复合物促进中心体微管的功能。  |  Bouguenina, H., et al. 2017. Proc Natl Acad Sci U S A. 114: E10687-E10696. PMID: 29162697
  4. PTPN22与EB1相互作用,调节T细胞受体信号传导。  |  Zhang, X., et al. 2020. FASEB J. 34: 8959-8974. PMID: 32469452
  5. EB1对小鼠卵母细胞减数分裂成熟过程中纺锤体的形成和染色体的排列至关重要  |  Zhou, D., et al. 2021. Microsc Microanal. 27: 385-391. PMID: 33413706
  6. EB1 通过 FAK 限制乳腺癌细胞内陷形成和基质蛋白水解  |  Chanez, B., et al. 2021. Cells. 10: PMID: 33668531
  7. 光遗传学EB1失活通过破坏皮质与星状微管的相互作用来缩短中期纺锤体。  |  Dema, A., et al. 2022. Curr Biol. 32: 1197-1205.e4. PMID: 35090591
  8. 用光敏感变体替换基因组后发现,生长锥的前进需要 EB1。  |  Dema, A., et al. 2023. Elife. 12: PMID: 36715499
  9. 在性传播阶段的染色体分离过程中,疟原虫ARK2和EB1驱动非常规纺锤体动力学。  |  Zeeshan, M., et al. 2023. Nat Commun. 14: 5652. PMID: 37704606
  10. 快速结合原纤维边缘位点有助于在生长中的微管加端追踪EB1的尖端。  |  Gonzalez, SJ., et al. 2024. Elife. 13: PMID: 38385657

订购信息

产品名称产品编号规格价格数量收藏夹

EB1 抗体 (F-7)

sc-374474
200 µg/ml
RMB2377.00

EB1 (F-7): m-IgGκ BP-HRP 套装

sc-552028
200 µg Ab; 40 µg BP
RMB2662.00

EB1 (F-7) 中和勝肽

sc-374474 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374474 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from i have performed Immunohistochemistry andi have performed Immunohistochemistry and western blotting in oral cancer tissue samples as well as immunofluoresence in oral cancer cell line. It works well.
Date published: 2015-10-31
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Robust signal in WB in HeLa whole cell lysateRobust signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Strong band in WB in SW480 whole cell lysateStrong band in WB in SW480 whole cell lysate. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Good specificity in WB in COLO320DM and HISMGood specificity in WB in COLO320DM and HISM whole cell lysates. -SCBT QC
Date published: 2015-01-10
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EB1 抗体 (F-7) is rated 5.0 out of 5 by 5.
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