Date published: 2025-9-7
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EB1 Antibody (1A11/4) - Western Blotting - Image 356672
EB1 抗体 (1A11/4): sc-47704. HEK293 (A), Jurkat (B), Ramos (C), A-431 (D) 和 HeLa (E) 全细胞裂解液及人脑组织提取物 (F) 中 EB1 表达的 Western 印迹分析.
EB1 抗体 (1A11/4): sc-47704. HEK293 (A), Jurkat (B), Ramos (C), A-431 (D) 和 HeLa (E) 全细胞裂解液及人脑组织提取物 (F) 中 EB1 表达的 Western 印迹分析.

EB1 抗体 (1A11/4): sc-47704

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寫評論提問

说明书
  • EB1 抗体 1A11/4 是小鼠单克隆 IgG2b κ，EB1抗体, 在23篇文献中引用，规格为200 µg/ml
  • 与 EB1 结合 δC1 突变体
  • EB1 抗体 (1A11/4) 推荐用于 WB, IP, IF 和 IHC(P)，检测human, canine 和monkey 来源的 EB1
  • 抗EB1抗体（1A11/4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EB1 (1A11/4): sc-47704的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 EB1 抗体 (1A11/4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EB1 抗体 (1A11/4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

EB1抗体（1A11/4）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人类、犬和猴的EB1蛋白。EB1 (1A11/4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。EB1，又称微管相关蛋白RP/EB家族成员1（MAPRE1）或APC结合蛋白EB1，在微管动力学中起着至关重要的作用，对于正常的细胞分裂和细胞内运输至关重要。MAPRE1的功能在癌症生物学中尤为重要，因为EB1可调节腺瘤性息肉病（APC）肿瘤抑制蛋白，从而预防结肠直肠癌。APC的突变会导致调控功能丧失，从而导致细胞无序增殖和肿瘤形成。EB1通过与APC的羧基末端区域结合，帮助调节肿瘤抑制作用，而MAPRE1表达失调与多种癌症有关，包括食管鳞状细胞癌。EB1对微管稳定性的影响以及与信号通路中其他蛋白的相互作用进一步强调了MAPRE1在正常细胞过程和癌症进展中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EB1 抗体 (1A11/4) 参考文献:

  1. EB1 蛋白调节微管动力学, 细胞极性和染色体稳定性。  |  Tirnauer, JS. and Bierer, BE. 2000. J Cell Biol. 149: 761-6. PMID: 10811817
  2. 家族性腺瘤性息肉病和散发性结肠癌中 APC 突变的分子分析。  |  Cottrell, S., et al. 1992. Lancet. 340: 626-30. PMID: 1355210
  3. EB1 介导的 APC 和 spectraplakins 向微管加端招募的结构决定因素。  |  Slep, KC., et al. 2005. J Cell Biol. 168: 587-98. PMID: 15699215
  4. CLIP-170 通过动态识别复合 EB1/微管蛋白结合位点，跟踪生长中的微管末端。  |  Bieling, P., et al. 2008. J Cell Biol. 183: 1223-33. PMID: 19103809
  5. CLIP-170 追踪微管加端需要 EB1。  |  Dixit, R., et al. 2009. Proc Natl Acad Sci U S A. 106: 492-7. PMID: 19126680
  6. 微管加端结合蛋白 EB1 是肌肉细胞分化, 伸长和融合所必需的。  |  Zhang, T., et al. 2009. J Cell Sci. 122: 1401-9. PMID: 19366726
  7. 黑色素瘤细胞的迁移和肌动蛋白突起受 EB1 蛋白调控。  |  Schober, JM., et al. 2009. Cancer Lett. 284: 30-6. PMID: 19427113
  8. EB1 直接调控 APC 介导的肌动蛋白成核。  |  Juanes, MA., et al. 2020. Curr Biol. 30: 4763-4772.e8. PMID: 33007249
  9. TIP60 对 EB1 的动态巴豆酰化确保了有丝分裂过程中纺锤体的准确定位。  |  Song, X., et al. 2021. Nat Chem Biol. 17: 1314-1323. PMID: 34608293
  10. EB1的相分离引导微管加端动力学。  |  Song, X., et al. 2023. Nat Cell Biol. 25: 79-91. PMID: 36536176
  11. APC 与新型蛋白质 EB1 结合。  |  Su, LK., et al. 1995. Cancer Res. 55: 2972-7. PMID: 7606712

订购信息

产品名称产品编号规格价格数量收藏夹

EB1 抗体 (1A11/4)

sc-47704
200 µg/ml
RMB2377.00

EB1 (1A11/4): m-IgG Fc BP-HRP 套装

sc-528509
200 µg Ab; 10 µg BP
RMB2662.00

EB1 (1A11/4): m-IgGκ BP-HRP 套装

sc-520903
200 µg Ab, 40 µg BP
RMB2662.00

EB1 (1A11/4): m-IgG2b BP-HRP 套装

sc-550048
200 µg Ab; 10 µg BP
RMB2662.00

EB1 抗体 (1A11/4) AC

sc-47704 AC
500 µg/ml, 25% agarose
RMB3129.00

EB1 抗体 (1A11/4) HRP

sc-47704 HRP
200 µg/ml
RMB2377.00

EB1 抗体 (1A11/4) FITC

sc-47704 FITC
200 µg/ml
RMB2482.00

EB1 抗体 (1A11/4) PE

sc-47704 PE
200 µg/ml
RMB2580.00

EB1 抗体 (1A11/4) Alexa Fluor® 488

sc-47704 AF488
200 µg/ml
RMB2685.00

EB1 抗体 (1A11/4) Alexa Fluor® 546

sc-47704 AF546
200 µg/ml
RMB2685.00

EB1 抗体 (1A11/4) Alexa Fluor® 594

sc-47704 AF594
200 µg/ml
RMB2685.00

EB1 抗体 (1A11/4) Alexa Fluor® 647

sc-47704 AF647
200 µg/ml
RMB2685.00

EB1 抗体 (1A11/4) Alexa Fluor® 680

sc-47704 AF680
200 µg/ml
RMB2685.00

EB1 抗体 (1A11/4) Alexa Fluor® 790

sc-47704 AF790
200 µg/ml
RMB2685.00

Will this antibody work for immunofluorescence protocols on Drosophila cells?

Asked by: Matthew Savoian
Thank you for your question. We recommend this antibody for human, canine and monkey . There is a 68% homology in Drosophila. We only recommend a species if the homology is 95% or higher . We recommend this antibody for WB, IP, PAR.
Answered by: BlakeJ
Date published: 2023-04-19

Can EB1 (1A11/4): sc-47704 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, EB1 (1A11/4): sc-47704 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!Great antibody for WB and IF on Jurkat T cells. Couldn't be better.
Date published: 2019-01-22
Rated 5 out of 5 by from Works very well in ImmunofluorescenceGood signal in RPE-1 cells, MetOH fixation, Can be used to distinguish dynamic microtubules.
Date published: 2018-03-27
Rated 4 out of 5 by from Good for IFThis EB1 Antibody (1A11/4) shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-12-04
Rated 5 out of 5 by from good antibodywork well in Hela A549 and MCF7 cellyste at the dilution of 1:100
Date published: 2017-10-24
Rated 5 out of 5 by from Great antibody for WB!It works really well in WB. Dilution 1:3000. The cell extracts were from human colon carcinoma cells.
Date published: 2017-10-19
Rated 5 out of 5 by from Fantastic EB1 Antibody!Great performance seen in Western Blot, IF and IHC. Works with paraffin-embedded sections. No additional bands in WB!
Date published: 2017-01-20
Rated 5 out of 5 by from Works well in IFWorks well in IF, can publish with it.
Date published: 2015-09-29
Rated 5 out of 5 by from Good publishable data in WB and siRNA inGood publishable data in WB and siRNA in human cervical cancer SiHa, HeLa & CaSki cell line. -SCBT Publication Review
Date published: 2015-06-03
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EB1 抗体 (1A11/4) is rated 4.9 out of 5 by 11.
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