Date published: 2025-9-6

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E2F-5 抗体 (MH-5): sc-968

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说明书
  • E2F-5抗体(MH-5)是小鼠单克隆IgG1 κ, 在25篇文献中引用,规格为200 µg/ml
  • human E2F-5 89-346的氨基酸之间的表位图谱
  • 推荐用于 mouse, rat 和 human 来源的E2F-5 WB, IP 和 IF检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是E2F-5 Antibody (MH-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与E2F-5 Antibody (MH-5) 打包提供(请参阅下面的订购信息)。

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関連項目

人类视网膜母细胞瘤基因产物似乎在细胞增殖的负调控中发挥重要作用。Rb的功能失活可以通过突变介导,或者作为与DNA肿瘤病毒编码的蛋白质相互作用的结果。在迄今为止描述的所有Rb关联中,Rb与转录因子E2F之间复合物的鉴定最直接地将Rb与细胞增殖的调控联系起来。E2F最初是通过其在腺病毒E2启动子转录激活中的作用而被鉴定的。在许多编码具有细胞周期G1和S期假定功能的蛋白质的基因的上游发现了与E2F结合位点同源的序列。E2F-1是一个更广泛的转录调节因子家族的成员,包括E2F-2、E2F-3、E2F-4、E2F-5、E2F-6和E2F-7,它们中的每一个都与第二种蛋白质DP-1形成异二聚体,形成一个"活性"的E2F转录调控复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2F-5 抗体 (MH-5) 参考文献:

  1. E2F-5的过度表达与病理基础表型和更差的临床结果相关。  |  Umemura, S., et al. 2009. Br J Cancer. 100: 764-71. PMID: 19259095
  2. 在肝癌中,常见E2F5过表达的潜在致癌作用。  |  Jiang, Y., et al. 2011. World J Gastroenterol. 17: 470-7. PMID: 21274376
  3. E2f4和E2f5对于男性生殖系统的发育至关重要。  |  Danielian, PS., et al. 2016. Cell Cycle. 15: 250-60. PMID: 26825228
  4. MYCN诱导的E2F5通过调节细胞周期进展促进神经母细胞瘤细胞增殖。  |  Liu, Y., et al. 2019. Biochem Biophys Res Commun. 511: 35-40. PMID: 30765227
  5. E2F5 通过调控 TFPI2, MMP-2 和 MMP-9 促进前列腺癌细胞的迁移和侵袭。  |  Karmakar, D., et al. 2020. Carcinogenesis. 41: 1767-1780. PMID: 32386317
  6. 在携带野生型TP53的乳腺癌细胞中,E2F5的敲除通过TP53依赖性途径诱导细胞死亡。  |  Inagaki, Y., et al. 2020. Oncol Rep. 44: 2241-2252. PMID: 33000282
  7. E2F5通过直接上调UBE2T的转录来促进胃癌的增殖和侵袭。  |  Li, L., et al. 2022. Dig Liver Dis. 54: 937-945. PMID: 34583905
  8. RNA结合蛋白IMP3的敲除通过破坏E2F5转录的稳定性抑制口腔鳞状细胞癌的增殖。  |  Wang, Z., et al. 2024. Aging (Albany NY). 16: 1897-1910. PMID: 38271139
  9. E2F-5是E2F家族的新成员,其分子和功能特征。  |  Buck, V., et al. 1995. Oncogene. 11: 31-8. PMID: 7542760
  10. E2F-5转录因子的分子和功能特征。  |  Vaishnav, YN., et al. 1998. Biochem Biophys Res Commun. 242: 586-92. PMID: 9464260

订购信息

产品名称产品编号规格价格数量收藏夹

E2F-5 抗体 (MH-5)

sc-968
200 µg/ml
RMB2377.00

E2F-5 (MH-5): m-IgG Fc BP-HRP 套装

sc-536545
200 µg Ab; 10 µg BP
RMB2662.00

E2F-5 (MH-5): m-IgGκ BP-HRP 套装

sc-533696
200 µg Ab; 40 µg BP
RMB2662.00

E2F-5 (MH-5): m-IgG1 BP-HRP 套装

sc-544667
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for sc-968: E2F-5 (MH-5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Strong staining in IHC in mouse NIH/3T3 cellsStrong staining in IHC in mouse NIH/3T3 cells. -SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from Good publishable data in EMSA and WB in human BGood publishable data in EMSA and WB in human B-lymphocytes. -SCBT Publication Review
Date published: 2015-03-29
Rated 4 out of 5 by from Single strong band in WB in NIH/3T3Single strong band in WB in NIH/3T3, MM-142 and and phorbol ester-induced MM-142 nuclear extracts. -SCBT QC
Date published: 2015-02-24
Rated 4 out of 5 by from Good specificity in WB in human MCFGood specificity in WB in human MCF-7 cells. -SCBT Publication Review
Date published: 2015-02-05
Rated 4 out of 5 by from Worked well in EMSA in mouse NIH/3T3 cellsWorked well in EMSA in mouse NIH/3T3 cells. -SCBT Publication Review
Date published: 2013-01-08
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E2F-5 抗体 (MH-5) is rated 4.0 out of 5 by 5.
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