Date published: 2025-9-6

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E2F-4 抗体 (RK-13): sc-511

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说明书
  • E2F-4抗体(RK-13)是小鼠单克隆IgG2a κ, 在15篇文献中引用,规格为200 µg/ml
  • 免疫human物种的E2F-4的氨基酸108-300
  • 抗-E2F-4 抗体 (RK-13) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 E2F-4
  • 抗E2F-4抗体(RK-13)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-511 X, 200 µg/0.1 ml)
  • 关于如何获取E2F-4 (RK-13): sc-511免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 E2F-4 抗体 (RK-13) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与E2F-4 抗体 (RK-13) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

E2F-4抗体(RK-13)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学(IHC)检测小鼠、大鼠和人类来源的E2F-4蛋白。E2F-4 (RK-13) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。E2F-4蛋白通过控制DNA合成和细胞增殖所需的基因表达,在调节细胞周期中发挥着至关重要的作用,特别是在G1期到S期的过渡中。这种调节对于维持正常的细胞生长和分裂至关重要,因为失调会导致细胞增殖失控,并导致肿瘤形成。E2F-4是一种转录因子,与DP-1蛋白形成异二聚体,形成活跃的E2F转录调控复合物,影响靶基因的表达。E2F-4在细胞周期调控中的重要性凸显了其作为癌症治疗靶标的潜力,在癌症治疗中,恢复正常的E2F活性有助于恢复正常的细胞周期控制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2F-4 抗体 (RK-13) 参考文献:

  1. 编码具有转录因子 E2F 特性的 pRB 结合蛋白的 cDNA。  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
  2. 视网膜母细胞瘤相关 pRb2/p130 与 E2F-4 和 -5 之间的相互作用与 JCV-TAg 的关系。  |  Caracciolo, V., et al. 2007. J Cell Physiol. 212: 96-104. PMID: 17385710
  3. E2F 转录因子是 RB 蛋白的细胞靶标。  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
  4. 视网膜母细胞瘤产物的 T/E1A 结合域可选择性地与序列特异性 DNA 结合蛋白相互作用。  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
  5. E2F转录因子4(E2F4)/p130(视网膜母细胞瘤基因家族成员之一)在心肌细胞中的抗凋亡功能。  |  Dingar, D., et al. 2012. J Mol Cell Cardiol. 53: 820-8. PMID: 22985930
  6. E2F4DN转基因小鼠:评估E2F4作为神经病理学和脑衰老治疗靶标的工具。  |  Ramón-Landreau, M., et al. 2022. Int J Mol Sci. 23: PMID: 36292945
  7. E2F4/5诱导的NF-κB诱导激酶的转录诱导促进了GBM细胞的集体侵袭。  |  Pflug, KM., et al. 2023. Sci Rep. 13: 13093. PMID: 37567906
  8. E2F-4是E2F转录因子家族的新成员,与p107相互作用。  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
  9. E2F-4是E2F基因家族的新成员,具有致癌活性,并在体内与p107结合。  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
  10. 与 DNA 和视网膜母细胞瘤基因产物的结合由不同 E2F 家族成员形成的复合物促进。  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
  11. 转录因子 E2F-1 和 DP-1 的异源二聚化导致了合作性的转激活。  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
  12. E2F-6 是 E2F 家族的成员,可作为转录抑制因子。  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

订购信息

产品名称产品编号规格价格数量收藏夹

E2F-4 抗体 (RK-13)

sc-511
200 µg/ml
RMB2377.00

E2F-4 (RK-13): m-IgG Fc BP-HRP 套装

sc-528117
200 µg Ab; 10 µg BP
RMB2662.00

E2F-4 (RK-13): m-IgGκ BP-HRP 套装

sc-520419
200 µg Ab, 40 µg BP
RMB2662.00

E2F-4 (RK-13): m-IgG2a BP-HRP 套装

sc-547027
200 µg Ab; 10 µg BP
RMB2662.00

E2F-4 抗体 (RK-13) X

sc-511 X
200 µg/0.1 ml
RMB2377.00

E2F-4 抗体 (RK-13) AC

sc-511 AC
500 µg/ml, 25% agarose
RMB3129.00

E2F-4 抗体 (RK-13) HRP

sc-511 HRP
200 µg/ml
RMB2377.00

E2F-4 抗体 (RK-13) FITC

sc-511 FITC
200 µg/ml
RMB2482.00

E2F-4 抗体 (RK-13) PE

sc-511 PE
200 µg/ml
RMB2580.00

E2F-4 抗体 (RK-13) Alexa Fluor® 488

sc-511 AF488
200 µg/ml
RMB2685.00

E2F-4 抗体 (RK-13) Alexa Fluor® 546

sc-511 AF546
200 µg/ml
RMB2685.00

E2F-4 抗体 (RK-13) Alexa Fluor® 594

sc-511 AF594
200 µg/ml
RMB2685.00

E2F-4 抗体 (RK-13) Alexa Fluor® 647

sc-511 AF647
200 µg/ml
RMB2685.00

E2F-4 抗体 (RK-13) Alexa Fluor® 680

sc-511 AF680
200 µg/ml
RMB2685.00

E2F-4 抗体 (RK-13) Alexa Fluor® 790

sc-511 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-511 X be used instead of the standard form sc-511?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western BlotWestern Blot analysis E2F-4 expressed in HEK 293T cells . Specific detection with a little background.Used diluted 1:800
Date published: 2017-08-26
Rated 5 out of 5 by from quality productI bought the E2F-4 antibody and it worked well, the band is very clear and beautiful.
Date published: 2017-03-28
Rated 5 out of 5 by from Saw good result in WB in mouse and human samplesSaw good result in WB in mouse and human samples.
Date published: 2015-09-23
Rated 5 out of 5 by from Great publishable EMSA data using this antibodyGreat publishable EMSA data using this antibody on mouse 3T3 cells. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Clean band achieved on mouse E2FClean band achieved on mouse E2F-4 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Good ChIP antibodyGood ChIP antibody, published using human MCF-7 and MDA-MB-231. -SCBT Publication Review
Date published: 2015-05-06
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic and membraneGreat immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human fallopian tube tissue. -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for IP with human MCF7, A549, RKO and RKO-E7 cells. -SCBT Publication Review
Date published: 2015-02-21
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E2F-4 抗体 (RK-13) is rated 4.8 out of 5 by 8.
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