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E2F-4 抗体 (WUF11): sc-56669

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说明书
  • E2F-4抗体(WUF11)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 针对全长E2F-4,human起源
  • 推荐用于 mouse, rat 和 human 来源的E2F-4 WB 和 IP检测
  • 看 E2F-4 (D-7): sc-398543,可查看有标记的 E2F-4 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是E2F-4 Antibody (WUF11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与E2F-4 Antibody (WUF11) 打包提供(请参阅下面的订购信息)。
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人视网膜母细胞瘤基因产物可能在细胞增殖的负调控中发挥重要作用。Rb的功能失活可以通过突变或由与DNA肿瘤病毒编码的蛋白质相互作用引起。在迄今为止描述的所有Rb关联中,Rb与转录因子E2F之间复合物的鉴定最直接地将Rb与细胞增殖的调控联系起来。E2F最初是通过其在腺病毒E2启动子的转录激活中的作用而被鉴定的。在许多编码细胞周期G1和S期假定功能的蛋白质的基因的上游发现了与E2F结合位点同源的序列。E2F-1是包括E2F-2、E2F-3、E2F-4、E2F-5、E2F-6和E2F-7在内的更广泛的转录调节因子家族的成员,每个成员都与第二种蛋白质DP-1形成异二聚体,形成"活性"的E2F转录调节复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2F-4 抗体 (WUF11) 参考文献:

  1. 侵袭性乳腺癌中E2F-4的表达与不良预后相关。  |  Rakha, EA., et al. 2004. J Pathol. 203: 754-61. PMID: 15221934
  2. E2F4在G2期发挥作用:维持G2期停滞,防止DNA受损时进入有丝分裂期。  |  Plesca, D., et al. 2007. Cell Cycle. 6: 1147-52. PMID: 17507799
  3. 对T7噬菌体展示肽库的筛选发现E2F-4是一种依托泊苷结合蛋白。  |  Takami, M., et al. 2011. Molecules. 16: 4278-94. PMID: 21610657
  4. p130/E2F4的稳定过度表达会影响骨髓来源间充质干细胞的多潜能性。  |  Zhang, X., et al. 2018. J Cell Physiol. 233: 9739-9749. PMID: 29987913
  5. NRF1、E2F4和MYC转录因子之间相互作用,调节共同靶基因,促进癌症发展和恶化。  |  Bhawe, K. and Roy, D. 2018. Cell Oncol (Dordr). 41: 465-484. PMID: 30047092
  6. Wnt/β-Catenin-p130/E2F4的激活通过细胞周期停滞促进骨髓来源间充质干细胞向II型肺泡上皮细胞分化。  |  Zhang, X., et al. 2023. Exp Ther Med. 26: 330. PMID: 37346406
  7. E2F-4在细胞周期重新进入时从p130切换到p107和pRB。  |  Moberg, K., et al. 1996. Mol Cell Biol. 16: 1436-49. PMID: 8657117
  8. E2F-1而非E2F-4能够克服p16诱导的G1细胞周期停滞。  |  Mann, DJ. and Jones, NC. 1996. Curr Biol. 6: 474-83. PMID: 8723352
  9. E2F4-RB和E2F4-p107复合物通过E2F结合位点抑制转化生长因子β的基因表达。  |  Li, JM., et al. 1997. Proc Natl Acad Sci U S A. 94: 4948-53. PMID: 9144170
  10. E2F-4 的亚细胞定位依赖于细胞周期。  |  Lindeman, GJ., et al. 1997. Proc Natl Acad Sci U S A. 94: 5095-100. PMID: 9144196

订购信息

产品名称产品编号规格价格数量收藏夹

E2F-4 抗体 (WUF11)

sc-56669
200 µg/ml
CNY2422.00

E2F-4 (WUF11): m-IgG Fc BP-HRP 套装

sc-537113
200 µg Ab; 10 µg BP
CNY2715.00

E2F-4 (WUF11): m-IgGκ BP-HRP 套装

sc-534305
200 µg Ab; 40 µg BP
CNY2715.00

E2F-4 (WUF11): m-IgG1 BP-HRP 套装

sc-545011
200 µg Ab; 20 µg BP
CNY2715.00

What is the recommended starting dilution for Western blot for sc-56669: E2F-4 (WUF11) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by SH from Strong band in WB IN Y79 nuclear extractStrong band in WB IN Y79 nuclear extract. -SCBT QC
Date published: 2015-05-14
Rated 4 out of 5 by QZ from Worked well in WB in mouse transfected 293TWorked well in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by SF from Good WB antibody in HLGood WB antibody in HL-60, Raji and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-09-06
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E2F-4 抗体 (WUF11) is rated 4.0 out of 5 by 3.
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