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DR5 Antibody (D-6) - Immunofluorescence - Image 145442
DR5 抗体 (D-6): sc-166624. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
DR5 抗体 (D-6): sc-166624. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.

DR5 抗体 (D-6): sc-166624

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说明书
  • DR5 抗体 D-6 是小鼠单克隆 IgG1 κ，DR5抗体, 在38篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的DR5的内部区域内的氨基酸48-76之间
  • DR5 抗体 (D-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 DR5
  • 抗DR5抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DR5 (D-6): sc-166624的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是DR5 Antibody (D-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DR5 Antibody (D-6) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外DR抗体，包括DR3, DR4, DR5和DR6

DR5抗体（D-6）是一种IgG1 κ小鼠单克隆DR5抗体（也被称为TNFRSF10B抗体、KILLER抗体、TRAILR2抗体或CD262抗体），它通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附测定法（ELISA）检测人类的DR5蛋白。DR5抗体（D-6）既可以是非偶联的抗DR5抗体形式，也可以是多种偶联形式的抗DR5抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。肿瘤坏死因子（TNF）是一种多效性细胞因子，其功能由两种不同的细胞表面受体介导，这两种受体被命名为TNF-R1和TNF-R2，它们在大多数细胞类型上都有表达。TNF的功能主要通过TNF-R1信号传导来介导。这两种受体都属于不断增长的TNF受体超家族，其中包括FAS抗原和CD40。TNF-R1包含一个细胞质基序，被称为"死亡结构域"，已被发现对于凋亡信号的传导是必需的。死亡结构域也在其他几种受体中发现，包括FAS、DR2（或TRUNDD）、DR3（死亡受体3）、DR4和DR5。TRUNDD、DR4和DR5是诱导凋亡的细胞因子TRAIL的受体。一种不含死亡结构域的受体，被命名为诱饵受体（DcR1或TRID），也与TRAIL特异性结合，并可能在细胞对凋亡刺激的抵抗中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DR5 抗体 (D-6) 参考文献:

  1. 用TRAIL和TRAIL-R2/DR5靶向瘢痕疙瘩  |  Sun, P., et al. 2021. J Dermatolog Treat. 32: 957-964. PMID: 31916474
  2. 在内质网应激时恢复TRAILR2/DR5介导的凋亡激活作为癌症的治疗策略  |  Mora-Molina, R. and López-Rivas, A. 2022. Int J Mol Sci. 23: PMID: 36012252
  3. 用肿瘤穿透性 iRGD 肽功能化的 DR5 选择性 TRAIL 变体 DR5-B，可增强对胶质母细胞瘤的抗肿瘤活性。  |  Yagolovich, AV., et al. 2022. Int J Mol Sci. 23: PMID: 36293545
  4. DR5二硫键是蛋白质折叠压力的传感器和效应器。  |  Law, ME., et al. 2024. bioRxiv.. PMID: 38496520
  5. 法斯死亡因素  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  6. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
  7. 55 kd TNF 受体中的一个新结构域发出细胞死亡信号。  |  Tartaglia, LA., et al. 1993. Cell. 74: 845-53. PMID: 8397073
  8. 一种含死亡域的受体，可介导细胞凋亡。  |  Kitson, J., et al. 1996. Nature. 384: 372-5. PMID: 8934525
  9. 细胞毒性配体 TRAIL 的受体。  |  Pan, G., et al. 1997. Science. 276: 111-3. PMID: 9082980
  10. 一种颉颃物诱饵受体和一种含死亡结构域的 TRAIL 受体。  |  Pan, G., et al. 1997. Science. 277: 815-8. PMID: 9242610
  11. 信号和诱饵受体家族控制 TRAIL 诱导的细胞凋亡。  |  Sheridan, JP., et al. 1997. Science. 277: 818-21. PMID: 9242611
  12. TRUNDD 是 TRAIL 受体家族的新成员，可颉颃 TRAIL 信号作用。  |  Pan, G., et al. 1998. FEBS Lett. 424: 41-5. PMID: 9537512

订购信息

产品名称产品编号规格价格数量收藏夹

DR5 抗体 (D-6)

sc-166624
200 µg/ml
RMB2377.00

DR5 (D-6): m-IgG Fc BP-HRP 套装

sc-529008
200 µg Ab; 10 µg BP
RMB2662.00

DR5 (D-6): m-IgGκ BP-HRP 套装

sc-521679
200 µg Ab, 40 µg BP
RMB2662.00

DR5 (D-6): m-IgG1 BP-HRP 套装

sc-543265
200 µg Ab; 20 µg BP
RMB2662.00

DR5 抗体 (D-6) AC

sc-166624 AC
500 µg/ml, 25% agarose
RMB3129.00

DR5 抗体 (D-6) HRP

sc-166624 HRP
200 µg/ml
RMB2377.00

DR5 抗体 (D-6) FITC

sc-166624 FITC
200 µg/ml
RMB2482.00

DR5 抗体 (D-6) PE

sc-166624 PE
200 µg/ml
RMB2580.00

DR5 抗体 (D-6) Alexa Fluor® 488

sc-166624 AF488
200 µg/ml
RMB2685.00

DR5 抗体 (D-6) Alexa Fluor® 546

sc-166624 AF546
200 µg/ml
RMB2685.00

DR5 抗体 (D-6) Alexa Fluor® 594

sc-166624 AF594
200 µg/ml
RMB2685.00

DR5 抗体 (D-6) Alexa Fluor® 647

sc-166624 AF647
200 µg/ml
RMB2685.00

DR5 抗体 (D-6) Alexa Fluor® 680

sc-166624 AF680
200 µg/ml
RMB2685.00

DR5 抗体 (D-6) Alexa Fluor® 790

sc-166624 AF790
200 µg/ml
RMB2685.00

DR5 (D-6) 中和勝肽

sc-166624 P
100 µg/0.5 ml
RMB511.00

I want to use DR5 (D-6): sc-166624 monoclonal antibody for Western blot. What is the recommended starting dilution for this antibody?

Asked by: cjMara
We recommend using DR5 (D-6): sc-166624 mouse monoclonal antibody at a starting dilution of 1:200 for Western blot (range of 1:100-1:1,000).
Answered by: Technical Support 15
Date published: 2017-01-20

Is it antibody targeting the internal or external part of the receptor? According to RCSB the epitope mapping between amino acids 48-76 falls in the extracellular part of the receptor?

Asked by: YHua
Thank you for your question. This antibody is specific for an epitope mapping between amino acids 48-76 within an internal region of DR5 of human origin and should detect the extracellular part of the DR5 (amino acids 56-444 are extracellular per accession#O14763). Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2016-10-24
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Rated 5 out of 5 by from 不错新用户，期待收货。希望合作愉快！售后、售前工作希望能洽谈愉快！之前在其他平台订货，第一次在这下单，期待
Date published: 2022-06-27
Rated 4 out of 5 by from Clear and good signalPrimary antibody DR5 (D-6) sc-166624 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membranes of PVDF have been incubated overnight at 4°C and then they have been probed for 1h at RT with secondary antibodies diluted 1:5000 in Milk 2,5%. We have tested this antibodiy on melanoma cell line A375 and A2058. In both cases we obtained a clear and quite good signal, so we can conclude that the antibody has worked very well.
Date published: 2020-11-09
Rated 1 out of 5 by from tested on rat cells (NRK52E)Tested in WB on NRK52E cell lysate + BJAB cell lysate (used as positive control) Expected MW at 48kDa We have observed 2 different bands in WB at 72kDa and 25kDa. Nothing at 48kda. Probably, it is possible that the antibody is not specific for rat cells. In any case, we have seen similar results also in BJAB cell lysate (no clear band at 48kDa).
Date published: 2020-08-10
Rated 1 out of 5 by from does not work for western in MDA-MB-231 cellsThis DR-5 antibody does not work for western in MDA-MB-231 cells
Date published: 2018-02-02
Rated 5 out of 5 by from Good antibody!I bought this antibody for Western blot experiments and the specific bands could be observed by using this antibody!
Date published: 2017-08-02
Rated 5 out of 5 by from GOOD!I got this antibody sample two weeks ago, and I am very glad that this antibody is not bad. I uesd DR5 of 1:100 dilution in WB and second antibody is 1:2000 dilution, loaded 60 ug.
Date published: 2017-05-31
Rated 5 out of 5 by from Good antibodyThe clean band was got with this antibody in western blot.
Date published: 2017-05-22
Rated 5 out of 5 by from Good WB resultsUsed it for WB experiment and worked perfectly. Good quality.
Date published: 2017-03-29
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DR5 抗体 (D-6) is rated 4.4 out of 5 by 14.
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