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DOT1L1 Antibody (E-5) - Western Blotting - Image 149974 — DOT1L1 抗体 (E-5): sc-374317. 非转染: sc-117752 (A) 和小鼠 DOT1L1 转染: sc-178530 (B) 293T 全细胞裂解液中 DOT1L1 表达的 Western 印迹分析.

DOT1L1 抗体 (E-5): sc-374317. 非转染: sc-117752 (A) 和小鼠 DOT1L1 转染: sc-178530 (B) 293T 全细胞裂解液中 DOT1L1 表达的 Western 印迹分析.

DOT1L1 抗体 (E-5): sc-374317

Name: DOT1L1抗体 (E-5)
Brand: SCBIO
SKU: sc-374317
Price: 2377.0 CNY
Availability: InStock

3.5(4)

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说明书

DOT1L1 抗体 E-5 是小鼠单克隆 IgG₁ (kappa轻链)，DOT1L1抗体，规格为200 µg/ml
免疫原氨基酸序列1-300位于human物种的DOT1L1的N-terminus
DOT1L1 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 DOT1L1
抗DOT1L1抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于ChIP的TransCruz试剂(sc-374317 X, 200 µg/0.1 ml)
关于如何获取DOT1L1 (E-5): sc-374317的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是DOT1L1 Antibody (E-5) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与DOT1L1 Antibody (E-5) 打包提供（请参阅下面的订购信息）。

快捷链接

DOT1L1 抗体 (E-5) 参考文献:

H3 赖氨酸 79 的甲基化是由不含 SET 结构域的 HMT 酶新家族介导的。 | Feng, Q., et al. 2002. Curr Biol. 12: 1052-8. PMID: 12123582
人 DOT1L（一种非 SET 结构域的核糖体组蛋白甲基转移酶）催化结构域的结构。 | Min, J., et al. 2003. Cell. 112: 711-23. PMID: 12628190
hDOT1L 将组蛋白甲基化与白血病的发生联系起来。 | Okada, Y., et al. 2005. Cell. 121: 167-78. PMID: 15851025
组蛋白 H3 赖氨酸-79 甲基转移酶对醛固酮敏感的 ENaCalpha 转录抑制作用 | Zhang, W., et al. 2006. Am J Physiol Cell Physiol. 290: C936-46. PMID: 16236820
Dot1a-AF9 复合物以醛固酮敏感的方式介导组蛋白 H3 Lys-79 超甲基化并抑制 ENaCalpha。 | Zhang, W., et al. 2006. J Biol Chem. 281: 18059-68. PMID: 16636056
CALM-AF10 的白血病转化涉及 hDOT1L 对 Hoxa5 的上调。 | Okada, Y., et al. 2006. Nat Cell Biol. 8: 1017-24. PMID: 16921363
在哺乳动物细胞中，DOT1L/KMT4的招募和H3K79甲基化与基因转录普遍相关。 | Steger, DJ., et al. 2008. Mol Cell Biol. 28: 2825-39. PMID: 18285465
反复出现的 SET-NUP214 融合是小儿 T 细胞急性淋巴细胞白血病中一种新的 HOXA 激活机制。 | Van Vlierberghe, P., et al. 2008. Blood. 111: 4668-80. PMID: 18299449
Dot1和H3K79甲基化的不同功能。 | Nguyen, AT. and Zhang, Y. 2011. Genes Dev. 25: 1345-58. PMID: 21724828
综合分析揭示了H3K79me2在介导可变剪接中的功能和调节作用。 | Li, T., et al. 2018. Genome Med. 10: 30. PMID: 29665865
计算模型揭示了端粒沉默1样干扰物（DOT1L）和参与白血病发生的复合物的主要分子特性和动态行为。 | Stodola, TJ., et al. 2022. Proteins. 90: 282-298. PMID: 34414607
白血病中的HOXA9调控组和药理干预。 | Aryal, S. and Lu, R. 2024. Adv Exp Med Biol. 1459: 405-430. PMID: 39017854

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
DOT1L1 抗体 (E-5)	sc-374317	200 µg/ml	RMB2377.00
DOT1L1 (E-5): m-IgG Fc BP-HRP 套装	sc-529720	200 µg Ab; 10 µg BP	RMB2662.00
DOT1L1 (E-5): m-IgGκ BP-HRP 套装	sc-522699	200 µg Ab, 40 µg BP	RMB2662.00
DOT1L1 (E-5): m-IgG₁ BP-HRP 套装	sc-543651	200 µg Ab; 20 µg BP	RMB2662.00
DOT1L1 抗体 (E-5) X	sc-374317 X	200 µg/0.1 ml	RMB2377.00
DOT1L1 抗体 (E-5) AC	sc-374317 AC	500 µg/ml, 25% agarose	RMB3129.00
DOT1L1 抗体 (E-5) HRP	sc-374317 HRP	200 µg/ml	RMB2377.00
DOT1L1 抗体 (E-5) FITC	sc-374317 FITC	200 µg/ml	RMB2482.00
DOT1L1 抗体 (E-5) PE	sc-374317 PE	200 µg/ml	RMB2580.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 488	sc-374317 AF488	200 µg/ml	RMB2685.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 546	sc-374317 AF546	200 µg/ml	RMB2685.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 594	sc-374317 AF594	200 µg/ml	RMB2685.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 647	sc-374317 AF647	200 µg/ml	RMB2685.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 680	sc-374317 AF680	200 µg/ml	RMB2685.00
DOT1L1 抗体 (E-5) Alexa Fluor^® 790	sc-374317 AF790	200 µg/ml	RMB2685.00

Hi I was looking to purchase an antibody for DOT1L, for use in immunoblotting and immunoprecipation. I saw the following promotion and would be interested to hear more. "Contact us to receive a FREE 10 µg sample of DOT1L1 (E-5): sc-374317".

Asked by: Janith

Thank you for your question. Please contact our Technical Service Department and we would be happy to help answer any questions you have about our current antibody sample promotion. https://www.scbt.com/scbt/resources/technical-service

Answered by: Technical Support

Date published: 2017-09-13

Are there any issues with using sc-374317: DOT1L1 (E-5) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-374317 conjugates.

Answered by: Technical Support

Date published: 2017-03-07

Rated 3 out of 5 by z0lei001 from A fair antibodyWestern blotting with this antibody in mouse testicular tissues did not detect any bands around molecular weight of 150 to 190 kDa.

Date published: 2017-08-25

Rated 5 out of 5 by IIRENE from Works greatExcellent antibody! One single (and strong) band at the right molecular weight.

Date published: 2017-07-21

Rated 1 out of 5 by my223 from Not good for IPIt cannot pull down itself in co-IP experiment using human cell nuclear extract.

Date published: 2017-06-24

Rated 5 out of 5 by MS from Good Western blot data of DOT1L1 expression in nonGood Western blot data of DOT1L1 expression in non-transfected and mouse DOT1L1 transfected 293T whole cell lysates. -SCBT QC

Date published: 2013-05-30

DOT1L1 抗体 (E-5) is rated 3.5 out of 5 by 4.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	DOT1L	Entrez Gene ID: 84444	19p13.3	XM_005259659, XM_005259660, XM_011528359, XM_011528360, XM_011528361, XM_017027366, NM_032482	UniProt ID: Q8TEK3	607375
Mouse	Dot1l	Entrez Gene ID: 208266	10 C1	XM_006513429, XM_006513430, XM_006513431, XM_006513432, XM_011243390, XM_011243391, NM_199322	UniProt ID: XP_006513492	N/A

DOT1L1 抗体 (E-5): sc-374317

DOT1L1 抗体 (E-5) 参考文献:

DOT1L1 抗体 (E-5) 规格

替代产品名称: DOT1L1

产品克隆名称: E-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 1-300

抗原表位靶标: DOT1L1

抗原表位种属: human

产品反应性/检测: DOT1L1

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 0篇各种科学出版物的引文

DOT1L1 抗体 (E-5)

DOT1L1 (E-5): m-IgG Fc BP-HRP 套装

DOT1L1 (E-5): m-IgGκ BP-HRP 套装

DOT1L1 (E-5): m-IgG₁ BP-HRP 套装

DOT1L1 抗体 (E-5) X

DOT1L1 抗体 (E-5) AC

DOT1L1 抗体 (E-5) HRP

DOT1L1 抗体 (E-5) FITC

DOT1L1 抗体 (E-5) PE

DOT1L1 抗体 (E-5) Alexa Fluor^® 488

DOT1L1 抗体 (E-5) Alexa Fluor^® 546

DOT1L1 抗体 (E-5) Alexa Fluor^® 594

DOT1L1 抗体 (E-5) Alexa Fluor^® 647

DOT1L1 抗体 (E-5) Alexa Fluor^® 680

DOT1L1 抗体 (E-5) Alexa Fluor^® 790

Hi I was looking to purchase an antibody for DOT1L, for use in immunoblotting and immunoprecipation. I saw the following promotion and would be interested to hear more. "Contact us to receive a FREE 10 µg sample of DOT1L1 (E-5): sc-374317".

Are there any issues with using sc-374317: DOT1L1 (E-5) mouse monoclonal primary antibody on mouse samples?

DOT1L1 抗体 (E-5): sc-374317

DOT1L1 抗体 (E-5) 参考文献:

DOT1L1 抗体 (E-5) 规格

替代产品名称: DOT1L1

产品克隆名称: E-5

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 1-300

抗原表位靶标: DOT1L1

抗原表位种属: human

产品反应性/检测: DOT1L1

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 0篇各种科学出版物的引文

DOT1L1 抗体 (E-5)

DOT1L1 (E-5): m-IgG Fc BP-HRP 套装

DOT1L1 (E-5): m-IgGκ BP-HRP 套装

DOT1L1 (E-5): m-IgG1 BP-HRP 套装

DOT1L1 抗体 (E-5) X

DOT1L1 抗体 (E-5) AC

DOT1L1 抗体 (E-5) HRP

DOT1L1 抗体 (E-5) FITC

DOT1L1 抗体 (E-5) PE

DOT1L1 抗体 (E-5) Alexa Fluor® 488

DOT1L1 抗体 (E-5) Alexa Fluor® 546

DOT1L1 抗体 (E-5) Alexa Fluor® 594

DOT1L1 抗体 (E-5) Alexa Fluor® 647

DOT1L1 抗体 (E-5) Alexa Fluor® 680

DOT1L1 抗体 (E-5) Alexa Fluor® 790

Hi I was looking to purchase an antibody for DOT1L, for use in immunoblotting and immunoprecipation. I saw the following promotion and would be interested to hear more. &quot;Contact us to receive a FREE 10 &micro;g sample of DOT1L1 (E-5): sc-374317&quot;.

Are there any issues with using sc-374317: DOT1L1 (E-5) mouse monoclonal primary antibody on mouse samples?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

DOT1L1 (E-5): m-IgG₁ BP-HRP 套装

DOT1L1 抗体 (E-5) Alexa Fluor^® 488

DOT1L1 抗体 (E-5) Alexa Fluor^® 546

DOT1L1 抗体 (E-5) Alexa Fluor^® 594

DOT1L1 抗体 (E-5) Alexa Fluor^® 647

DOT1L1 抗体 (E-5) Alexa Fluor^® 680

DOT1L1 抗体 (E-5) Alexa Fluor^® 790

Hi I was looking to purchase an antibody for DOT1L, for use in immunoblotting and immunoprecipation. I saw the following promotion and would be interested to hear more. "Contact us to receive a FREE 10 µg sample of DOT1L1 (E-5): sc-374317".