Date published: 2025-9-9
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Dicer Antibody (A-2) - Western Blotting - Image 70462
Dicer 抗体 (A-2): sc-136981. 在 HeLa (A), Jurkat (B), 293 (C), U-2 OS (D), K-562 (E), RPE-J (F) 全细胞裂解液和 Hep G2 核提取物 (G) 中 Dicer 表达的 Western 印迹分析.
Dicer 抗体 (A-2): sc-136981. 在 HeLa (A), Jurkat (B), 293 (C), U-2 OS (D), K-562 (E), RPE-J (F) 全细胞裂解液和 Hep G2 核提取物 (G) 中 Dicer 表达的 Western 印迹分析.

Dicer 抗体 (A-2): sc-136981

4.7(6)
寫評論提問

说明书
  • Dicer抗体(A-2)是小鼠单克隆IgG1 κ, 在11篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1701-1912，在Dicer的human起源的C-terminus处进行映射
  • 抗-Dicer 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dicer
  • 抗Dicer抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dicer (A-2): sc-136981的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Dicer 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dicer 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Dicer 抗体包括 Dicer-1

Dicer抗体（A-2）是一种IgG1 κ小鼠单克隆Dicer抗体（也称为Dicer抗体或DICER1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的Dicer。Dicer抗体（A-2）既可用作非偶联形式的抗Dicer抗体，也可用作多种偶联形式的抗Dicer抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。哺乳动物的Dicer是一种与III型RNase相关的蛋白质，在酵母、果蝇和拟南芥中具有同源物。Dicer在其氨基末端含有一个RNA解旋酶基序，包括一个DEXH盒，在其羧基末端含有一个RNase基序。编码人类Dicer的基因位于14q31染色体上。Dicer在脑、心、肝、肺、胰、肾和胎盘中均有表达，并在RNA干扰途径中发挥作用。Dicer将大约70bp的短发夹RNA前体切割成21-23bp的dsRNAs，这些dsRNAs选择性地靶向同源RNA的破坏。Dicer定位于哺乳动物细胞的细胞质中。具体来说，它与内质网中的钙网蛋白共定位。尽管Dicer对RNA的切割不依赖于ATP，但这种酶快速周转所需的产物释放可能归因于ATP。免疫沉淀研究表明，Dicer与eIF2C翻译起始因子的PIWI结构域形成复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Dicer 抗体 (A-2)

sc-136981
200 µg/ml
RMB2377.00

Dicer (A-2): m-IgG Fc BP-HRP 套装

sc-528833
200 µg Ab; 10 µg BP
RMB2662.00

Dicer (A-2): m-IgGκ BP-HRP 套装

sc-521381
200 µg Ab, 40 µg BP
RMB2662.00

Dicer (A-2): m-IgG1 BP-HRP 套装

sc-543160
200 µg Ab; 20 µg BP
RMB2662.00

Dicer 抗体 (A-2) AC

sc-136981 AC
500 µg/ml, 25% agarose
RMB3129.00

Dicer 抗体 (A-2) HRP

sc-136981 HRP
200 µg/ml
RMB2377.00

Dicer 抗体 (A-2) FITC

sc-136981 FITC
200 µg/ml
RMB2482.00

Dicer 抗体 (A-2) PE

sc-136981 PE
200 µg/ml
RMB2580.00

Dicer 抗体 (A-2) Alexa Fluor® 488

sc-136981 AF488
200 µg/ml
RMB2685.00

Dicer 抗体 (A-2) Alexa Fluor® 546

sc-136981 AF546
200 µg/ml
RMB2685.00

Dicer 抗体 (A-2) Alexa Fluor® 594

sc-136981 AF594
200 µg/ml
RMB2685.00

Dicer 抗体 (A-2) Alexa Fluor® 647

sc-136981 AF647
200 µg/ml
RMB2685.00

Dicer 抗体 (A-2) Alexa Fluor® 680

sc-136981 AF680
200 µg/ml
RMB2685.00

Dicer 抗体 (A-2) Alexa Fluor® 790

sc-136981 AF790
200 µg/ml
RMB2685.00

Can this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, this utrophin antibody sc-136981 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 5 out of 5 by from Good feature!I applyed for the sample of this antibody for free. I performed immunohistochemistry of FFPE (formalin fixed and paraffin embedded) with this antibody (diluted 1:50) to detect the expression of dicer in human breast tissues. IHC, diluted 1:50, overnight, Human breast tissue.
Date published: 2018-04-17
Rated 5 out of 5 by from Excellent Dicer antibody!This Dicer (A-2) monoclonal antibody is an excellent choice for Western blot, and has been featured in 5 publications.
Date published: 2016-12-22
Rated 5 out of 5 by from Published using this antibody in WesternPublished using this antibody in Western Blot using MRC5SV, ATM-/-, M059J, 180BRM and HeLa cells. Used to confirm knockdown by siRNA.
Date published: 2015-06-15
Rated 4 out of 5 by from Strong band seen in WB using LPS/IFNStrong band seen in WB using LPS/IFN- stimulated RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-05-16
Rated 4 out of 5 by from Publishable data in WB using mouse ES cellsPublishable data in WB using mouse ES cells -SCBT Publication Review
Date published: 2015-03-19
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-03-09
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Dicer 抗体 (A-2) is rated 4.7 out of 5 by 6.
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