Date published: 2025-9-7
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DHFR Antibody (F-8) - Western Blotting - Image 362103
DHFR 抗体 (F-8): sc-74594. Jurkat (A), HeLa (B), NIH/3T3 (C) 和 3T3-L1 (D) 全细胞裂解液中 DHFR 表达的 Western 印迹分析.
DHFR 抗体 (F-8): sc-74594. Jurkat (A), HeLa (B), NIH/3T3 (C) 和 3T3-L1 (D) 全细胞裂解液中 DHFR 表达的 Western 印迹分析.

DHFR 抗体 (F-8): sc-74594

4.3(4)
寫評論提問

说明书
  • DHFR抗体(F-8)是小鼠单克隆IgG2a κ, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长DHFR的氨基酸1-187
  • 推荐用于 mouse, rat 和 human 来源的DHFR WB, IP, IF 和 ELISA检测
  • 看 DHFR (A-9): sc-377091，可查看有标记的 DHFR 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 2a BP-HRP">m-IgG2a BP-HRP是DHFR Antibody (F-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与DHFR Antibody (F-8) 打包提供（请参阅下面的订购信息）。

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関連項目

二氢叶酸还原酶（DHFR）催化二氢叶酸在NADPH依赖的方式下还原为四氢叶酸，是嘌呤、嘧啶和一些氨基酸合成的关键酶。该酶活性的抑制会导致DNA合成停止和细胞死亡。在正常造血细胞中表达甲氨蝶呤（MTX）抗性的DHFR变异体基因，是一种潜在的策略，这种策略可以通过减轻药物敏感的正常细胞和组织中的药物毒性，从而允许使用更大剂量的MTX。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

DHFR 抗体 (F-8)

sc-74594
200 µg/ml
RMB2377.00

DHFR (F-8): m-IgG2a BP-HRP 套装

sc-546012
200 µg Ab; 10 µg BP
RMB2662.00

Are there any issues with using sc-74594: DHFR (F-8) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-74594 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-05-29
Rated 4 out of 5 by from Strong bands seen in WB using HeLa and KNRKStrong bands seen in WB using HeLa and KNRK whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 4 out of 5 by from Band seen in WB using human recombinant DHFRBand seen in WB using human recombinant DHFR. -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from IP and WB publishable data with human EAIP and WB publishable data with human EA.hy926 cells - SCBT Publication Review
Date published: 2013-06-07
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DHFR 抗体 (F-8) is rated 4.3 out of 5 by 4.
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