How much sample do you recommend loading on the gel for Western blot detection using DHFR (49): sc-136246?
Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference.
https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting