Date published: 2025-9-7

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DDB1 抗体 (B-1): sc-137142

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说明书
  • DDB1抗体(B-1)是小鼠单克隆IgG2a κ, 在3篇文献中引用,规格为200 µg/ml
  • 免疫human物种的DDB1的氨基酸1-300
  • 推荐用于 mouse, rat 和 human 来源的DDB1 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是DDB1 Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DDB1 Antibody (B-1) 打包提供(请参阅下面的订购信息)。

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関連項目

受损DNA结合蛋白(DDB)是由两个亚基p127和p48组成的异二聚体,分别被命名为DDB1和DDB2。DDB异二聚体参与修复被紫外线损伤的DNA。具体来说,DDB,也被称为紫外线损伤DNA结合蛋白(UV-DDB)、着色性干皮病E组结合因子(XPE-BF)和乙型肝炎病毒X相关蛋白1(XAP-1),与受损的环丁烷嘧啶二聚体(CPDs)结合。DDB2基因的突变被认为是着色性干皮病E组的原因,这是一种常染色体隐性遗传病,其中患者在核苷酸切除DNA修复方面存在缺陷。XPE的特点是皮肤对阳光高度敏感,皮肤癌发病率高,以及神经系统异常。乙型肝炎病毒(HBV)X蛋白与DDB1相互作用,这可能介导HBx的反式激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DDB1 抗体 (B-1) 参考文献:

  1. DDB1 通过与 HBx 无关的机制刺激乙型肝炎病毒的转录物  |  Kim, W., et al. 2016. J Virol. 90: 9644-9653. PMID: 27535046
  2. DDB1 介导的 CRY1 降解促进肝脏中 FOXO1 驱动的糖元生成  |  Tong, X., et al. 2017. Diabetes. 66: 2571-2582. PMID: 28790135
  3. DDB1 E3连接酶通过CRY1控制饮食果糖诱导的ChREBPα稳定和肝脏脂肪变性。  |  Tong, X., et al. 2020. Metabolism. 107: 154222. PMID: 32246987
  4. SALL4-泊马度胺-脑隆-DB1复合物的晶体结构。  |  Matyskiela, ME., et al. 2020. Nat Struct Mol Biol. 27: 319-322. PMID: 32251415
  5. DDB1 通过防止细胞周期停滞来维持肠道稳态。  |  Zhao, L., et al. 2022. Cell Regen. 11: 18. PMID: 35641707
  6. 鉴定 CUL4A-DDB1-WDFY1 作为 E3 泛素连接酶复合物参与溶酶体的启动。  |  Teranishi, H., et al. 2022. Cell Rep. 40: 111349. PMID: 36103833
  7. 脉冲-SILAC和相互作用组学揭示了不同的DDB1-CUL4相关因子、细胞功能和蛋白质底物  |  Raisch, J., et al. 2023. Mol Cell Proteomics. 22: 100644. PMID: 37689310
  8. 肝细胞特异性缺失 DDB1 可减轻肝脂肪变性,但会加重 MASH 的肝脏炎症和纤维化。  |  Gu, Q., et al. 2024. Hepatol Commun. 8: PMID: 38934719
  9. 发现和设计 CDK12-DDB1 相互作用的分子胶增强剂,以定向降解细胞周期蛋白 K。  |  Ghosh, P., et al. 2024. RSC Chem Biol. 6: 36-55. PMID: 39450271
  10. DDB1 可使棕色脂肪细胞为冷诱导的产热做好准备。  |  Wang, X., et al. 2022. Life Metab. 1: 39-53. PMID: 39872690

订购信息

产品名称产品编号规格价格数量收藏夹

DDB1 抗体 (B-1)

sc-137142
200 µg/ml
RMB2377.00

DDB1 (B-1): m-IgG Fc BP-HRP 套装

sc-537409
200 µg Ab; 10 µg BP
RMB2662.00

DDB1 (B-1): m-IgGκ BP-HRP 套装

sc-534664
200 µg Ab; 40 µg BP
RMB2662.00

DDB1 (B-1): m-IgG2a BP-HRP 套装

sc-548008
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with DDB1 (B-1): sc-137142 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Performs well in WesternPerforms well in Western, strong signal with mouse DDB1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from Strong positive band observed in WB in humanStrong positive band observed in WB in human DDB1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from CleanClean, crisp band detected in WB in UV irradiated HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-22
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DDB1 抗体 (B-1) is rated 5.0 out of 5 by 4.
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