Date published: 2025-9-9
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DBH Antibody (A-9) - Western Blotting - Image 295717
DBH 抗体 (A-9): sc-365710. SH-SY5Y (A) 和 BE (2)-M17 (B) 全细胞裂解液中 DBH 表达的 Western 印迹分析.
DBH 抗体 (A-9): sc-365710. SH-SY5Y (A) 和 BE (2)-M17 (B) 全细胞裂解液中 DBH 表达的 Western 印迹分析.

DBH 抗体 (A-9): sc-365710

4.8(4)
寫評論提問

说明书
  • DBH抗体(A-9)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列391-603位于human物种的DBH的C-terminus附近
  • DBH 抗体 (A-9) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 DBH
  • 抗DBH抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DBH (A-9): sc-365710的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是DBH Antibody (A-9) for WB applications. 的首选辅助检测试剂，这些试剂现与DBH Antibody (A-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

DBH抗体（A-9）是一种IgG1 κ小鼠单克隆DBH抗体，可通过WB、IP、IF和ELISA检测人源DBH。DBH抗体（A-9）既可作为非偶联抗DBH抗体形式，也可作为多种偶联形式的抗DBH抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。多巴胺β-羟化酶（DBH）在儿茶酚胺的生物合成中催化多巴胺向去甲肾上腺素的转化。DBH在去甲肾上腺素能神经元和肾上腺素能神经元以及神经内分泌细胞中选择性表达，是去甲肾上腺素能过程的特异性蛋白标志物。DBH的活性形式是同源四聚体，存在于相应神经细胞的突触囊泡腔内，定位于细胞膜和细胞质中。DBH受神经生长因子和胰岛素生长因子-1的诱导，并受细胞内第二信使蛋白激酶A、环AMP、二酰甘油和Ca2+的调节。DBH的表达在转录上由Sp1、CREB和AP-1蛋白介导，包括c-Fos、c-Jun和JunD。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

DBH 抗体 (A-9)

sc-365710
200 µg/ml
RMB2377.00

DBH (A-9): m-IgG Fc BP-HRP 套装

sc-527394
200 µg Ab; 10 µg BP
RMB2662.00

DBH (A-9): m-IgG1 BP-HRP 套装

sc-532767
200 µg Ab; 20 µg BP
RMB2662.00

DBH 抗体 (A-9) AC

sc-365710 AC
500 µg/ml, 25% agarose
RMB3129.00

DBH 抗体 (A-9) HRP

sc-365710 HRP
200 µg/ml
RMB2377.00

DBH 抗体 (A-9) FITC

sc-365710 FITC
200 µg/ml
RMB2482.00

DBH 抗体 (A-9) PE

sc-365710 PE
200 µg/ml
RMB2580.00

DBH 抗体 (A-9) Alexa Fluor® 488

sc-365710 AF488
200 µg/ml
RMB2685.00

DBH 抗体 (A-9) Alexa Fluor® 546

sc-365710 AF546
200 µg/ml
RMB2685.00

DBH 抗体 (A-9) Alexa Fluor® 594

sc-365710 AF594
200 µg/ml
RMB2685.00

DBH 抗体 (A-9) Alexa Fluor® 647

sc-365710 AF647
200 µg/ml
RMB2685.00

DBH 抗体 (A-9) Alexa Fluor® 680

sc-365710 AF680
200 µg/ml
RMB2685.00

DBH 抗体 (A-9) Alexa Fluor® 790

sc-365710 AF790
200 µg/ml
RMB2685.00

What species is this antibody reactive with?

Asked by: Anonymous
Thank you for your question. DBH Antibody (A-9): sc-365710 is only recommended for detection of DBH of human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2021-03-09

What is the recommended starting dilution for Western blot for sc-365710: DBH (A-9) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Good IF stainingI have succesfully used the antibody (lot. E2417) on adult heart criosections. It works well at dilution 1:50.
Date published: 2020-05-19
Rated 5 out of 5 by from Nice Western Blot data of DBH expression in nonNice Western Blot data of DBH expression in non-transfected and human DBH transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Worked greatWorked great!
Date published: 2015-03-23
Rated 5 out of 5 by from Nice Western Blot data of DBH expression in SHNice Western Blot data of DBH expression in SH-SY5Y and BE 2 -M17 whole cell lysates. -SCBT QC
Date published: 2015-02-18
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DBH 抗体 (A-9) is rated 4.8 out of 5 by 4.
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