Date published: 2026-10-26

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Cytokeratin 18 抗体 (DC-10): sc-6259

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说明书
  • Cytokeratin 18抗体(DC-10)是小鼠单克隆IgG1 κ, 在114篇文献中引用,规格为200 µg/ml
  • 针对PMC-42 human乳腺癌细胞
  • Cytokeratin 18 抗体 (DC-10) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 Cytokeratin 18
  • 抗Cytokeratin 18抗体(DC-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Cytokeratin 18 Antibody (DC-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Cytokeratin 18 Antibody (DC-10) 打包提供(请参阅下面的订购信息)。
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细胞角蛋白18抗体(DC-10)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测人类来源的细胞角蛋白18。抗细胞角蛋白18抗体(DC-10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。细胞角蛋白18是上皮细胞细胞骨架的重要组成部分,在维持细胞完整性和提供机械支撑方面发挥着关键作用。细胞角蛋白18的表达在上皮细胞分化和组织分化中尤为重要,有助于在压力下稳定细胞结构。细胞角蛋白18是多种癌症(包括腺癌和基底细胞癌)的生物标记,因此细胞角蛋白18(DC-10)抗体在癌症研究和诊断中具有重大价值。细胞角蛋白18在翻译后经历了显著的修饰,包括特定丝氨酸残基的磷酸化,这对于与14-3-3蛋白的相互作用至关重要。这种磷酸化对于细胞内角蛋白丝的正确组织和分布至关重要,进一步强调了细胞角蛋白18在细胞结构和功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 18 抗体 (DC-10) 参考文献:

  1. 活化半胱天冬酶-3和裂解细胞角蛋白18的免疫组织化学与TUNEL法在PC-3皮下异种移植组织切片中的细胞凋亡定量比较。  |  Duan, WR., et al. 2003. J Pathol. 199: 221-8. PMID: 12533835
  2. 非酒精性脂肪性肝炎中细胞角蛋白-18片段和代谢综合征的生物标志物。  |  Yilmaz, Y., et al. 2009. World J Gastroenterol. 15: 4387-91. PMID: 19764089
  3. 细胞角蛋白18在癌症中的生物学功能。  |  Weng, YR., et al. 2012. Mol Cancer Res. 10: 485-93. PMID: 22452884
  4. 细胞角蛋白18是乳腺上皮细胞中TGF-β1诱导的上皮-间质转化启动所必需的。  |  Jung, H., et al. 2016. Mol Cell Biochem. 423: 21-28. PMID: 27734227
  5. 肝硬化患者肝癌诊断中的细胞角蛋白-18。  |  Ismail, SA., et al. 2017. Asian Pac J Cancer Prev. 18: 1105-1111. PMID: 28547948
  6. 细胞角蛋白18相关组蛋白3在肝细胞癌中的调节:小型综述。  |  Lai, YC., et al. 2017. Cancer Genomics Proteomics. 14: 219-223. PMID: 28647696
  7. 作为发热性中性粒细胞减少症血液病患者预后生物标志物的血清caspase-裂解细胞角蛋白-18片段  |  Intke, C., et al. 2022. Clin Exp Med. 22: 83-93. PMID: 34255216
  8. 细胞角蛋白-18作为药物性肝损伤生物标志物的应用。  |  Korver, S., et al. 2021. Arch Toxicol. 95: 3435-3448. PMID: 34322741
  9. 血浆细胞角蛋白-18片段水平反映了肥胖的代谢表型。  |  Goralska, J., et al. 2023. Biomolecules. 13: PMID: 37189422
  10. 内脏肥胖和细胞角蛋白-18抗原作为肝损伤的早期生物标志物。  |  de Alteriis, G., et al. 2023. Int J Mol Sci. 24: PMID: 37446065

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 18 抗体 (DC-10)

sc-6259
200 µg/ml
CNY2422.00

Cytokeratin 18 (DC-10): m-IgG Fc BP-HRP 套装

sc-528165
200 µg Ab; 10 µg BP
CNY2715.00

Cytokeratin 18 (DC-10): m-IgGκ BP-HRP 套装

sc-520473
200 µg Ab, 40 µg BP
CNY2715.00

Cytokeratin 18 (DC-10): m-IgG1 BP-HRP 套装

sc-542795
200 µg Ab; 20 µg BP
CNY2715.00

Cytokeratin 18 抗体 (DC-10) AC

sc-6259 AC
500 µg/ml, 25% agarose
CNY3189.00

Cytokeratin 18 抗体 (DC-10) HRP

sc-6259 HRP
200 µg/ml
CNY2422.00

Cytokeratin 18 抗体 (DC-10) FITC

sc-6259 FITC
200 µg/ml
CNY2527.00

Cytokeratin 18 抗体 (DC-10) PE

sc-6259 PE
200 µg/ml
CNY2625.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 488

sc-6259 AF488
200 µg/ml
CNY2738.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 546

sc-6259 AF546
200 µg/ml
CNY2738.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 594

sc-6259 AF594
200 µg/ml
CNY2738.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 647

sc-6259 AF647
200 µg/ml
CNY2738.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 680

sc-6259 AF680
200 µg/ml
CNY2738.00

Cytokeratin 18 抗体 (DC-10) Alexa Fluor® 790

sc-6259 AF790
200 µg/ml
CNY2738.00

您好,我想问一下,该抗体可以用于小鼠的IHC嘛?

Asked by: Zhenshan
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the best secondary to use with this Cytokeratin 18 Antibody (DC-10): sc-6259?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by Anonymous from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by HappyFlower from Great antibody for western blotThe batch of antibody I have worked well on HeLa and MCF7 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 5 out of 5 by biofengtian from Very nice for prostate cell linesWorks well in western for prostate cancer cell lines with a negative result from RWPE1 cells.
Date published: 2017-10-13
Rated 5 out of 5 by xu cc from usefulThe antibody was great, the band looks clean, Good potency
Date published: 2017-08-21
Rated 5 out of 5 by Bill28 from Wow !! OutstandingVery strong,specific western blot results; also superb for IHC and IF
Date published: 2017-01-27
Rated 5 out of 5 by DefinitelyNotMatt from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-13
Rated 5 out of 5 by little J from Specific goodHalf a month ago I received our antibodies,Then do the immunohistochemical,Good antibody specificity, color soon.very good!!!
Date published: 2017-01-12
Rated 5 out of 5 by LR from Positive immunoperoxidase staining of formalinPositive immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tumor showing cytoskeletal staining of hepatocytes. -SCBT QC
Date published: 2015-04-06
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Cytokeratin 18 抗体 (DC-10) is rated 5.0 out of 5 by 12.
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