Date published: 2025-9-6
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Cytokeratin 18 Antibody (DA-7) - Western Blotting - Image 12562
细胞角蛋白 18 抗体 (DA-7): sc-51583. T24 (A) 和 HeLa (B) 全细胞裂解液中细胞角蛋白 18 表达的 Western 印迹分析.
细胞角蛋白 18 抗体 (DA-7): sc-51583. T24 (A) 和 HeLa (B) 全细胞裂解液中细胞角蛋白 18 表达的 Western 印迹分析.

Cytokeratin 18 抗体 (DA-7): sc-51583

4.4(5)
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说明书
  • Cytokeratin 18 抗体 DA-7 是小鼠单克隆 IgG1, 在5篇文献中引用，提供 100 µg/ml
  • 针对human乳腺癌细胞系PMC-42
  • 推荐用于 human 来源的Cytokeratin 18 WB, IP, IF 和 IHC(P)检测
  • 看 Cytokeratin 18 (RGE53): sc-32329，可查看有标记的 Cytokeratin 18 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Cytokeratin 18 抗体 (DA-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 Cytokeratin 抗体 包括 Basic Cytokeratin, Cytokeratin 1, Cytokeratin 10, Cytokeratin 12, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17和Cytokeratin 18

细胞角蛋白是一类多样的中间纤维蛋白（IFP），在角质化和非角质化的上皮组织中以成对的形式表达。细胞角蛋白在分化和组织特化中发挥着重要作用，并起到维持上皮细胞整体结构完整性的作用。细胞角蛋白被发现可以作为组织分化的有用标记，这直接适用于恶性肿瘤的表征。例如，细胞角蛋白10和13在一些鳞状细胞癌中表达较高，而细胞角蛋白18则在大多数腺癌和基底细胞癌中表达。细胞角蛋白18在Ser33和Ser52上具有两个主要的磷酸化位点。细胞角蛋白18的Ser18磷酸化对于细胞角蛋白18与14-3-3蛋白的结合是必需的，并参与角蛋白的组织和分布。

仅限研究使用。不适用于诊断和治疗用途。

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Cytokeratin 18 抗体 (DA-7)

sc-51583
100 µg/ml
RMB2377.00

Can sc-51583: Cytokeratin 18 (DA-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-51583: Cytokeratin 18 (DA-7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good specificity and signal strength in T24Good specificity and signal strength in T24 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Positive Western blot analysis of CytokeratinPositive Western blot analysis of Cytokeratin 18 expression in SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-02-28
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-01-03
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Cytokeratin 18 抗体 (DA-7) is rated 4.4 out of 5 by 5.
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