Date published: 2026-10-26

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Cytokeratin 18 抗体 (DA-7): sc-51583

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说明书
  • Cytokeratin 18 抗体 DA-7 是小鼠单克隆 IgG1, 在5篇文献中引用,提供 100 µg/ml
  • 针对human乳腺癌细胞系PMC-42
  • 推荐用于 human 来源的Cytokeratin 18 WB, IP, IF 和 IHC(P)检测
  • 看 Cytokeratin 18 (RGE53): sc-32329,可查看有标记的 Cytokeratin 18 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Cytokeratin 18 抗体 (DA-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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细胞角蛋白是一类多样的中间纤维蛋白(IFP),在角质化和非角质化的上皮组织中以成对的形式表达。细胞角蛋白在分化和组织特化中发挥着重要作用,并起到维持上皮细胞整体结构完整性的作用。细胞角蛋白被发现可以作为组织分化的有用标记,这直接适用于恶性肿瘤的表征。例如,细胞角蛋白10和13在一些鳞状细胞癌中表达较高,而细胞角蛋白18则在大多数腺癌和基底细胞癌中表达。细胞角蛋白18在Ser33和Ser52上具有两个主要的磷酸化位点。细胞角蛋白18的Ser18磷酸化对于细胞角蛋白18与14-3-3蛋白的结合是必需的,并参与角蛋白的组织和分布。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 18 抗体 (DA-7) 参考文献:

  1. 酒精性肝病中的血清细胞角蛋白:细胞角蛋白18和细胞角蛋白19水平的对比。  |  Gonzalez-Quintela, A., et al. 2006. Alcohol. 38: 45-9. PMID: 16762691
  2. 胃肠道腺癌患者血浆中的细胞角蛋白18作为肿瘤反应的生物标志物。  |  Scott, LC., et al. 2009. Br J Cancer. 101: 410-7. PMID: 19603019
  3. 裂解的细胞角蛋白-18是特发性肺纤维化的机理信息生物标志物。  |  Cha, SI., et al. 2012. Respir Res. 13: 105. PMID: 23167970
  4. 肝炎患者血清细胞角蛋白-18水平升高的临床价值:一项荟萃分析。  |  Yang, ZH., et al. 2015. Hepat Mon. 15: e25328. PMID: 26045704
  5. 血清细胞角蛋白-18与非酒精性脂肪肝疾病的进展或消退之间的相关性。  |  Kawanaka, M., et al. 2015. Ann Hepatol. 14: 837-44. PMID: 26436355
  6. 血清细胞角蛋白18水平与血液透析患者的饮食摄入和血清甘油三酯水平有关。  |  Haghighat, N., et al. 2020. Saudi J Kidney Dis Transpl. 31: 353-359. PMID: 32394907
  7. 血清细胞角蛋白18、癌胚抗原和甲状腺球蛋白在甲状腺乳头状癌患者中的诊断价值。  |  Yan, G., et al. 2021. Clin Lab. 67: PMID: 33616340
  8. MicroRNA-325-3p通过靶向细胞角蛋白18促进结直肠癌的发生。  |  Song, C., et al. 2021. Oncol Lett. 21: 248. PMID: 33664812
  9. 尿骨素、细胞角蛋白-18和内皮素在先天性阻塞性肾病患儿肾纤维化诊断中的实用性。  |  Turczyn, A., et al. 2021. J Clin Med. 10: PMID: 34768419
  10. 在扑热息痛治疗剂量的安慰剂对照、随机、交叉试验(PATH-BP 生物标志物子研究)中,细胞角蛋白-18 是丙氨酸转氨酶升高的敏感生物标志物。  |  Scullion, KM., et al. 2024. Toxicol Sci. 199: 203-209. PMID: 38521541

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 18 抗体 (DA-7)

sc-51583
100 µg/ml
CNY2422.00

Can sc-51583: Cytokeratin 18 (DA-7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-51583: Cytokeratin 18 (DA-7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by JL from Good specificity and signal strength in T24Good specificity and signal strength in T24 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 4 out of 5 by MS from Performs well in WesternPerforms well in Western, good signal strength in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by JL from Positive Western blot analysis of CytokeratinPositive Western blot analysis of Cytokeratin 18 expression in SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-02-28
Rated 5 out of 5 by LM from Works well for IF applicationsWorks well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-02-22
Rated 4 out of 5 by SF from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-01-03
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Cytokeratin 18 抗体 (DA-7) is rated 4.4 out of 5 by 5.
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