Date published: 2026-10-26

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Cytokeratin 18 抗体 (B23.1.): sc-58727

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说明书
  • Cytokeratin 18抗体(B23.1.)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 针对human来源的PMC-42乳腺癌细胞
  • 推荐用于 human 来源的Cytokeratin 18 WB, IP, IF, IHC(P) 和 FCM检测
  • 看 Cytokeratin 18 (RGE53): sc-32329,可查看有标记的 Cytokeratin 18 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 1 BP-HRP">m-IgG1 BP-HRP是Cytokeratin 18 Antibody (B23.1.) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Cytokeratin 18 Antibody (B23.1.) 打包提供(请参阅下面的订购信息)。
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细胞角蛋白由一组不同的中间丝蛋白(IFPs)组成,在角质化和非角质化上皮组织中成对表达。细胞角蛋白在分化和组织特化中起着关键作用,并起着维持上皮细胞整体结构完整性的作用。细胞角蛋白已被发现是组织分化的有用标记,可直接用于恶性肿瘤的表征。例如,细胞角蛋白10和13在鳞状细胞癌的亚群中高度表达,而细胞角蛋白18在大多数腺癌和基底细胞癌中表达。细胞角蛋白18在Ser 33和Ser 52上含有两个主要的磷酸化位点。 Ser 18的磷酸化对于细胞角蛋白18与14-3-3蛋白的结合至关重要,并参与角蛋白的组织和分布。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 18 抗体 (B23.1.) 参考文献:

  1. 纤维蛋白、细胞角蛋白8和细胞角蛋白18不是人类腭扁桃体M细胞的特异性标记物。  |  Koshi, R., et al. 2001. J Anat. 199: 663-74. PMID: 11787820
  2. 细胞角蛋白18作为膀胱癌肿瘤标志物的诊断价值。  |  Ramazan Sekeroğlu, M., et al. 2002. Clin Biochem. 35: 327-31. PMID: 12135697
  3. 细胞角蛋白18片段水平作为减肥手术患者非酒精性脂肪性肝炎的无创生物标志物。  |  Diab, DL., et al. 2008. Clin Gastroenterol Hepatol. 6: 1249-54. PMID: 18995215
  4. 通过蛋白质组分析发现细胞角蛋白 18 是鼻咽癌的新型生物标记物。  |  Li, XM., et al. 2009. J Cancer Res Clin Oncol. 135: 1763-75. PMID: 19529960
  5. 细胞角蛋白18是牛肠M细胞的一种特异性标记物。  |  Hondo, T., et al. 2011. Am J Physiol Gastrointest Liver Physiol. 300: G442-53. PMID: 21193527
  6. 细胞角蛋白18不是发育中前列腺形态发生所必需的,但有助于成人前列腺再生。  |  Zhang, C., et al. 2013. Biomed Res Int. 2013: 576472. PMID: 24672777
  7. 釉质上皮和牙源性囊肿中细胞角蛋白14和细胞角蛋白18的表达减少:在卵泡型腺瘤样牙源性肿瘤的癌胚转化和组织发生中可能起作用。  |  Shruthi, DK., et al. 2014. J Oral Maxillofac Pathol. 18: 365-71. PMID: 25948990
  8. 非幸存者缺血性脑卒中患者血清Caspase-裂解细胞角蛋白-18水平较高  |  Lorente, L., et al. 2020. Brain Sci. 10: PMID: 32120809
  9. 细胞角蛋白18(M30和M65)在检测非酒精性脂肪性肝炎和纤维化中的准确性:系统评价和荟萃分析。  |  Lee, J., et al. 2020. PLoS One. 15: e0238717. PMID: 32915852
  10. 细胞角蛋白18在人类肿瘤中的表达对诊断和预后的影响:一项针对11952例肿瘤的组织微阵列研究。  |  Menz, A., et al. 2021. Mol Med. 27: 16. PMID: 33588765

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 18 抗体 (B23.1.)

sc-58727
200 µg/ml
$322.00

Cytokeratin 18 (B23.1.): m-IgG1 BP-HRP 套装

sc-551944
200 µg Ab; 20 µg BP
$361.00

Can Cytokeratin 18 (B23.1.): sc-58727 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, Cytokeratin 18 (B23.1.): sc-58727 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by AB from Single strong band at correct mwSingle strong band at correct mw, MCF7. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by MS from Great staining with paraffinGreat staining with paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by FL from Good specificity and signal strength in HepGood specificity and signal strength in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by JT from Clean blot with single strong band at expectedClean blot with single strong band at expected size using in non-transfected and human Cytokeratin 18 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-01-22
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Cytokeratin 18抗体 (B23.1.) is rated 5.0 out of 5 by 4.
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