Date published: 2025-9-7
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cytochrome c Antibody (7H8) - Western Blotting - Image 329
细胞色素 c 抗体 (7H8): sc-13560. HL-60 (A), K-562 (B) 和 HeLa (C) 全细胞裂解液中细胞色素 c 表达的 Western 印迹分析.
细胞色素 c 抗体 (7H8): sc-13560. HL-60 (A), K-562 (B) 和 HeLa (C) 全细胞裂解液中细胞色素 c 表达的 Western 印迹分析.

cytochrome c 抗体 (7H8): sc-13560

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说明书
  • cytochrome c抗体(7H8)是小鼠单克隆IgG2b κ, 在267篇文献中引用，规格为200 µg/ml
  • 针对human来源的全长变性cytochrome c
  • 抗-cytochrome c 抗体 (7H8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human, equine 和avian 来源的 cytochrome c
  • cytochrome c 抗体 (7H8) 可偶联 琼脂糖 (sc-13560 AC), 500 µg/ml, 25% agarose, 用于 IP; 还可偶联 藻红蛋白 (sc-13560 PE) 或者 FITC (sc-13560 FITC), 200 µg/ml, 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cytochrome c (7H8): sc-13560的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 cytochrome c 抗体 (7H8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cytochrome c 抗体 (7H8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytochrome 抗体 包括 cytochrome b5 type B

细胞色素c抗体（7H8）是一种IgG2bκ型小鼠单克隆细胞色素c抗体，可通过WB、IP、IF和IHC（P）检测来自小鼠、大鼠和人类的细胞色素c。细胞色素c抗体（7H8）有偶联和非偶联的抗细胞色素c抗体形式。细胞色素c是一种特征明确的可移动电子转运蛋白，对所有需氧生物的能量转换至关重要。在哺乳动物细胞中，这种高度保守的蛋白质通常位于线粒体膜间空间。最近的研究已经确定了胞质细胞色素c作为激活凋亡所需的因子。在凋亡过程中，细胞色素c从线粒体膜转移到胞质中，在那里它激活了caspase-3（CPP32）。研究表明Bcl-2的过表达可以阻止细胞色素c的转位，从而阻断凋亡过程。Bax的过表达已被证明能诱导细胞色素c的释放并诱导细胞死亡。线粒体中细胞色素c的释放被认为触发凋亡级联反应，其中Apaf-1以细胞色素c依赖的方式与Apaf-3（caspase-9）结合，导致caspase-9切割caspase-3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

cytochrome c 抗体 (7H8)

sc-13560
200 µg/ml
RMB2377.00

cytochrome c (7H8): m-IgG Fc BP-HRP 套装

sc-525480
200 µg Ab; 10 µg BP
RMB2662.00

cytochrome c (7H8): m-IgG2b BP-HRP 套装

sc-549566
200 µg Ab; 10 µg BP
RMB2662.00

cytochrome c 抗体 (7H8) AC

sc-13560 AC
500 µg/ml, 25% agarose
RMB3129.00

cytochrome c 抗体 (7H8) FITC

sc-13560 FITC
200 µg/ml
RMB2482.00

cytochrome c 抗体 (7H8) PE

sc-13560 PE
200 µg/ml
RMB2580.00

cytochrome c 抗体 (7H8) Alexa Fluor® 488

sc-13560 AF488
200 µg/ml
RMB2685.00

cytochrome c 抗体 (7H8) Alexa Fluor® 546

sc-13560 AF546
200 µg/ml
RMB2685.00

cytochrome c 抗体 (7H8) Alexa Fluor® 594

sc-13560 AF594
200 µg/ml
RMB2685.00

cytochrome c 抗体 (7H8) Alexa Fluor® 647

sc-13560 AF647
200 µg/ml
RMB2685.00

cytochrome c 抗体 (7H8) Alexa Fluor® 680

sc-13560 AF680
200 µg/ml
RMB2685.00

cytochrome c 抗体 (7H8) Alexa Fluor® 790

sc-13560 AF790
200 µg/ml
RMB2685.00

Hello, I'm a researcher at UC Berkeley and interested in your antibody. We'd like to use this antibody (sc-13560) for IF in rat neurons. Could you send us a free sample to test? Thank you very much.

Asked by: Rui Yan
Thank you for your question. This mouse monoclonal antibody, cytochrome c (7H8): sc-13560, is recommended for detection of cytochrome c of rat origin and suitable for immunofluorescence (IF). We would be happy to send you a free 10 µg sample of the antibody to test before you place your order. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-21

Is there any data showing this antibody is specific to cytochrome C?

Asked by: AbPolly
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to cytochrome C.
Answered by: Technical Support 12
Date published: 2023-11-02
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Rated 5 out of 5 by from Stato fortunato grazie a voiVorrei comprare un appartamento posto più tranquillo devono stare bene lì con il mio famiglia senza turbare a nessuno
Date published: 2024-02-16
Rated 5 out of 5 by from it worked well !I recommend this antibody as we used it for our recent publication and it worked very well.
Date published: 2020-09-09
Rated 4 out of 5 by from Good antibody for WBI have used this antibody in MKN45 cancer cell line by WB and it worked pretty well. Plus, this served as a negative control for extracellular vesicles research (PMID: 31236201).
Date published: 2020-06-23
Rated 5 out of 5 by from Great antibodyThe antibody worked really nice for western blot in SCC-4 cells.
Date published: 2019-07-18
Rated 1 out of 5 by from didn't workSadly it didn't work as we planned. The dillution was 1/250 but we have to try more WB to see if it works
Date published: 2019-04-01
Rated 5 out of 5 by from good antibodyI always trust santa cruz. This antibody works well!
Date published: 2018-07-10
Rated 5 out of 5 by from Fine for WBTested in SHSY-5Y human neuroblastoma cells (~15ug/lane), dilution 1:200 in 3% milk in TBST. Got a single clear band at expected size.
Date published: 2018-05-15
Rated 4 out of 5 by from Good antibodyworked as specified, showed a single band, good antibody!
Date published: 2018-04-02
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cytochrome c抗体 (7H8) is rated 4.7 out of 5 by 23.
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