Date published: 2025-9-7
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cytochrome c Antibody (A-8) - Western Blotting - Image 396198
细胞色素 c 抗体 (A-8) Alexa Fluor® 647: sc-13156 AF647. RAW 264. 7 (A), M1 (B), NIH/3T3 (C), K-562 (D) 和 HL-60 (E) 全细胞裂解液中细胞色素 c 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
细胞色素 c 抗体 (A-8) Alexa Fluor® 647: sc-13156 AF647. RAW 264. 7 (A), M1 (B), NIH/3T3 (C), K-562 (D) 和 HL-60 (E) 全细胞裂解液中细胞色素 c 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

cytochrome c 抗体 (A-8): sc-13156

4.8(60)
寫評論提問

说明书
  • cytochrome c 抗体 A-8 是小鼠单克隆 IgG2b κ，cytochrome c抗体, 在565篇文献中引用，规格为200 µg/ml
  • 免疫equine物种的cytochrome c的氨基酸1-104
  • cytochrome c 抗体 (A-8) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 cytochrome c; 也和以下物种反应，包括： equine
  • 抗cytochrome c抗体（A-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cytochrome c (A-8): sc-13156的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 cytochrome c 抗体 (A-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cytochrome c 抗体 (A-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cytochrome 抗体 包括 cytochrome b5 type B

细胞色素c抗体（A-8）是一种小鼠单克隆IgG2b kappa轻链抗体，针对马细胞色素c的氨基酸1-104，可与小鼠、大鼠和人类蛋白反应。细胞色素c，也称为CYCS或体细胞色素c，是一种位于线粒体膜间空间的小血红素蛋白。这种定位使细胞色素c在电子传递链中发挥关键作用，在复合体III（细胞色素bc1复合体）和复合体IV（细胞色素c氧化酶）之间传递电子，这对氧化磷酸化过程中ATP的产生至关重要。细胞色素c在 线粒体，参与细胞凋亡的内在途径；释放到细胞质中后，细胞色素c与细胞凋亡蛋白酶激活因子-1（Apaf-1）相互作用，形成细胞凋亡复合物，激活半胱天冬酶-9和下游效应半胱天冬酶，如半胱天冬酶-3。线粒体释放细胞色素c是细胞程序性死亡的关键事件，这一过程的失调与癌症和神经退行性疾病等疾病有关。细胞色素c单克隆抗体（A-8）适用于包括蛋白质印迹、免疫沉淀、免疫荧光、石蜡包埋切片免疫组织化学、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）在内的各种应用。研究人员利用细胞色素抗-c抗体（A-8）研究线粒体功能、细胞凋亡机制和相关细胞过程，从而深入了解能量代谢和细胞死亡途径，这对于理解各种生理和病理状况至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cytochrome c 抗体 (A-8) 参考文献:

  1. 细胞色素 c 氧化酶中的质子动力协同作用  |  Papa, S., et al. 2004. Biochim Biophys Acta. 1658: 95-105. PMID: 15282180
  2. 细胞色素c从线粒体释放的机制。  |  Garrido, C., et al. 2006. Cell Death Differ. 13: 1423-33. PMID: 16676004
  3. 金黄色葡萄球菌对凝血酶诱导的血小板杀微生物蛋白-1（tPMP-1）的体外敏感性受细胞膜磷脂组成和不对称性的影响。  |  Mukhopadhyay, K., et al. 2007. Microbiology (Reading). 153: 1187-1197. PMID: 17379728
  4. 线粒体 c 型细胞色素的生物生成。  |  Gonzales, DH. and Neupert, W. 1990. J Bioenerg Biomembr. 22: 753-68. PMID: 1965436
  5. 细胞色素c在气凝胶中的稳定化和固定化。  |  Harper-Leatherman, AS., et al. 2017. Methods Mol Biol. 1504: 149-163. PMID: 27770420
  6. 细胞色素C氧化酶与类固醇激素的相互作用。  |  Oleynikov, IP., et al. 2020. Cells. 9: PMID: 33003582
  7. 在无细胞提取物中诱导细胞凋亡程序：需要 dATP 和细胞色素 c。  |  Liu, X., et al. 1996. Cell. 86: 147-57. PMID: 8689682
  8. Bcl-2 阻止细胞凋亡：阻止细胞色素 c 从线粒体释放。  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
  9. 线粒体释放细胞色素 c：Bcl-2 调控细胞凋亡的主要场所。  |  Kluck, RM., et al. 1997. Science. 275: 1132-6. PMID: 9027315
  10. Apaf-1是一种与优雅子CED-4同源的人类蛋白质，它参与细胞色素c依赖性的caspase-3活化。  |  Zou, H., et al. 1997. Cell. 90: 405-13. PMID: 9267021
  11. 细胞色素 c 和 dATP 依赖性 Apaf-1/caspase-9 复合物的形成启动了凋亡蛋白酶级联反应。  |  Li, P., et al. 1997. Cell. 91: 479-89. PMID: 9390557
  12. Bcl-2 可在 Bax 诱导细胞色素 c 释放后延长细胞存活时间。  |  Rossé, T., et al. 1998. Nature. 391: 496-9. PMID: 9461218

订购信息

产品名称产品编号规格价格数量收藏夹

cytochrome c 抗体 (A-8)

sc-13156
200 µg/ml
RMB2377.00

cytochrome c (A-8): m-IgG Fc BP-HRP 套装

sc-528275
200 µg Ab; 10 µg BP
RMB2662.00

cytochrome c (A-8): m-IgGκ BP-HRP 套装

sc-520614
200 µg Ab, 40 µg BP
RMB2662.00

cytochrome c (A-8): m-IgG2b BP-HRP 套装

sc-550020
200 µg Ab; 10 µg BP
RMB2662.00

cytochrome c 抗体 (A-8) AC

sc-13156 AC
500 µg/ml, 25% agarose
RMB3129.00

cytochrome c 抗体 (A-8) HRP

sc-13156 HRP
200 µg/ml
RMB2377.00

cytochrome c 抗体 (A-8) FITC

sc-13156 FITC
200 µg/ml
RMB2482.00

cytochrome c 抗体 (A-8) PE

sc-13156 PE
200 µg/ml
RMB2580.00

cytochrome c 抗体 (A-8) Alexa Fluor® 488

sc-13156 AF488
200 µg/ml
RMB2685.00

cytochrome c 抗体 (A-8) Alexa Fluor® 546

sc-13156 AF546
200 µg/ml
RMB2685.00

cytochrome c 抗体 (A-8) Alexa Fluor® 594

sc-13156 AF594
200 µg/ml
RMB2685.00

cytochrome c 抗体 (A-8) Alexa Fluor® 647

sc-13156 AF647
200 µg/ml
RMB2685.00

cytochrome c 抗体 (A-8) Alexa Fluor® 680

sc-13156 AF680
200 µg/ml
RMB2685.00

cytochrome c 抗体 (A-8) Alexa Fluor® 790

sc-13156 AF790
200 µg/ml
RMB2685.00

Does this antibody recognize both apocytochrome c and holocytochrome c?

Asked by: summer12
Thank you for your question. cytochrome c Antibody (A-8): sc-13156 has been raised against amino acids 1-104 of cytochrome c of equine origin and recognizes its target protein based on that sequence. Since this sequence is present in both holo- and apocytochrome c, the antibody should be able to detect those variants as well.
Answered by: Tech Support Europe
Date published: 2024-03-28

Please advise if this antibody (sc-13156) will work on frozen sections from rabbit eyes

Asked by: Paula Yu
Thank you for your question. Cytochrome c Antibody (A-8): sc-13156 has not been tested in rabbit cells or tissues, so we do not have any data on whether or not it would work in rabbit eye frozen sections. Please contact our Technical Service directly to arrange for a sample of this antibody if you would like to try it in your experimental setup.
Answered by: Tech Support Europe
Date published: 2024-03-28

What is the recommended secondary reagent for cytochrome c Antibody (A-8) in Western blot applications when working with mouse tissue samples?

Asked by: Trav11
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2024-07-09
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Rated 5 out of 5 by from The Cytochrome C Ab worked very well in WBCytochrome C antibody worked very well in WB using human samples
Date published: 2024-05-30
Rated 5 out of 5 by from Great for WBI bought this antibody a few years ago and the result is good and clear.
Date published: 2024-03-20
Rated 4 out of 5 by from AgradecidoMe pareció un servicio estupendo a la par que interesante
Date published: 2024-02-21
Rated 4 out of 5 by from Western blotsThe antibody provides clear signals without high background by western blots using several human cell lines.
Date published: 2022-12-05
Rated 5 out of 5 by from 免疫印迹申请了小样试试，做的是western，结果很好。以前用的是另一家的抗体，质量也不错，但价格更不错，所以性价比来讲，推荐santa的
Date published: 2020-12-20
Rated 4 out of 5 by from buon funzionamentosciolto in latte secondo sheet, ottima resa e chiarezza della banda.
Date published: 2020-11-09
Rated 5 out of 5 by from cytochrome c (A-8) SAMPLEcytochrome c (A-8) SAMPLE worked great for mouse cytochrome C detection by western blot!
Date published: 2020-01-29
Rated 4 out of 5 by from goodCan see the band, it is necessary to have a higher concentration of sample protein. protein was extracted from HeLa cell
Date published: 2019-09-06
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cytochrome c抗体 (A-8) is rated 4.8 out of 5 by 60.
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