Date published: 2025-9-8

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CYPOR 抗体 (G-5): sc-25263

5.0(3)
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说明书
  • CYPOR抗体(G-5)是小鼠单克隆IgG2a κ, 在5篇文献中引用,规格为200 µg/ml
  • 免疫human物种的cytochrome P450 reductase的氨基酸1-300
  • 推荐用于 human 来源的CYPOR WB, IP, IF 和 ELISA检测
  • 目前,我们还没有完成CYPOR 抗体 (G-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

P450酶是一类单氧化酶,参与代谢多种内源性和异源性化合物。几种P450酶根据序列相似性被归类为CYP1A和CYP2A亚家族的成员。CYPOR,也称为细胞色素P450还原酶和NADPH细胞色素P450还原酶,是一种微粒体酶,负责将电子从NADPH转移到细胞色素P450酶,以完成P450催化周期。CYPOR定位于内质网,在那里也能将电子转移到血红蛋白氧化酶和细胞色素b5。CYPOR在结构上与两个独立的黄素蛋白家族,还原硫醇核苷酸还原酶(FNR)和黄素蛋白相关。CYPOR的电子转移需要两种黄素辅因子FAD和FMN分别结合到FNR和黄素蛋白结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYPOR 抗体 (G-5) 参考文献:

  1. 细胞色素P450还原酶介导的乙醇厌氧生物转化,生成1-羟基乙基自由基和乙醛。  |  Díaz Gómez, MI., et al. 2000. Toxicology. 154: 113-22. PMID: 11118675
  2. 人细胞色素P450还原酶的电子转移。  |  Gutierrez, A., et al. 2003. Biochem Soc Trans. 31: 497-501. PMID: 12773143
  3. 微粒体细胞色素P450 2B4与其氧化还原伴侣细胞色素P450还原酶和细胞色素b(5)的相互作用。  |  Im, SC. and Waskell, L. 2011. Arch Biochem Biophys. 507: 144-53. PMID: 21055385
  4. 细胞色素P450氧化还原酶(CYPOR)对间隙连接功能和连接蛋白43表达的调节。  |  Polusani, SR., et al. 2011. Biochem Biophys Res Commun. 411: 490-5. PMID: 21726529
  5. 7-脱氢胆固醇还原酶活性与细胞色素P450还原酶无关。  |  Zou, L., et al. 2011. J Steroid Biochem Mol Biol. 127: 435-8. PMID: 21762780
  6. 抑制细胞色素P450还原酶的表达可促进成年小鼠脑室下区星形胶质细胞增生。  |  Yao, Y., et al. 2013. Neurosci Lett. 548: 84-9. PMID: 23727388
  7. 通过中子散射确定的细胞色素P450还原酶-细胞色素c复合物的溶液结构。  |  Freeman, SL., et al. 2018. J Biol Chem. 293: 5210-5219. PMID: 29475945
  8. 用于体外生物转化研究的人肝细胞和HepG2细胞中的NADPH-细胞色素P450还原酶表达和酶活性。  |  Schulz, C., et al. 2019. Clin Hemorheol Microcirc. 73: 249-260. PMID: 31561354
  9. 通过整合生物信息学工具和功能分析,从基因组聚合数据库中探索细胞色素P450还原酶基因(POR)的新变体。  |  Rojas Velazquez, MN., et al. 2023. Biomolecules. 13: PMID: 38136599
  10. 细胞色素P450还原酶的膜结合段和FMN结构域之间由8个氨基酸组成的柔性连接子对于最佳活性是必需的。  |  Rwere, F., et al. 2024. J Inorg Biochem. 259: 112667. PMID: 39032346

订购信息

产品名称产品编号规格价格数量收藏夹

CYPOR 抗体 (G-5)

sc-25263
200 µg/ml
$316.00

What is the recommended fixation method for immunofluorescence staining with CYPOR (G-5): sc-25263 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice antibody for CYPOR detectionWe used this antibody for CYPOR expression detection with western blot. We got a nice band at the correct molecular weight for HeLa cells
Date published: 2017-02-01
Rated 5 out of 5 by from WB publishable data with human THPWB publishable data with human THP-1 and JEG-3 cells - SCBT Publication Review
Date published: 2015-01-20
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-08-26
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CYPOR 抗体 (G-5) is rated 5.0 out of 5 by 3.
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