Date published: 2025-9-7
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CYP4V2 Antibody (M29-P3B10) - Western Blotting - Image 47832
CYP4V2 抗体 (M29-P3B10): sc-101386. 人肝微粒体中 CYP4V2 表达的 Western 印迹分析.
CYP4V2 Antibody (M29-P3B10) - Western Blotting - Image 47832
CYP4V2 抗体 (M29-P3B10): sc-101386. 人肝微粒体中 CYP4V2 表达的 Western 印迹分析.
CYP4V2 抗体 (M29-P3B10): sc-101386. 人肝微粒体中 CYP4V2 表达的 Western 印迹分析.

CYP4V2 抗体 (M29-P3B10): sc-101386

4.7(6)
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说明书
  • CYP4V2 抗体 M29-P3B10 是小鼠单克隆 IgG1 (kappa轻链)，CYP4V2抗体，规格为200 µg/ml
  • 与human的CYP4V2的氨基酸495-504对应的合成肽杂交
  • CYP4V2 抗体 (M29-P3B10) 推荐用于WB, IP 和 ELISA，检测human 来源的 CYP4V2
  • 抗CYP4V2抗体（M29-P3B10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP4V2 (M29-P3B10): sc-101386的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CYP4V2 Antibody (M29-P3B10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CYP4V2 Antibody (M29-P3B10) 打包提供（请参阅下面的订购信息）。

    快捷链接

    相关产品
    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    额外CYP抗体，包括CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1, CYP27B1, CYP2A6, CYP2B1, CYP2C6, CYP2D6, CYP2F1和CYP2F2

    CYP4V2抗体（M29-P3B10）是一种IgG1 κ小鼠单克隆CYP4V2抗体（也被指定为CYP4V2抗体），可通过WB、IP和ELISA检测人源CYP4V2蛋白。CYP4V2抗体（M29-P3B10）既可作为非偶联的抗CYP4V2抗体形式，也可作为多种偶联形式的抗CYP4V2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CYP4V2（细胞色素P450 4V2）是一种包含525个氨基酸的蛋白质，属于细胞色素P450家族。它定位于内质网膜，并在各种组织中广泛表达。CYP4V2的缺陷与Bietti结晶状角膜视网膜营养不良（BCD）有关，这是一种常染色体隐性视网膜营养不良，其特征是眼底散布着多个闪烁的视网膜内晶体，视网膜特征性退化和脉络膜血管硬化，最终导致进行性夜盲和视野收缩。CYP4V2与其他细胞色素P450家族成员的同源性表明，它可能在脂肪酸和类固醇代谢中发挥作用。由于可选择的剪接事件，存在两种CYP4V2异构体。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    CYP4V2 抗体 (M29-P3B10) 参考文献:

    1. Bietti 晶状体角膜营养不良症与染色体 4q35 的遗传联系。  |  Jiao, X., et al. 2000. Am J Hum Genet. 67: 1309-13. PMID: 11001583
    2. Bietti 晶体性角膜营养不良症是由新型基因 CYP4V2 突变引起的。  |  Li, A., et al. 2004. Am J Hum Genet. 74: 817-26. PMID: 15042513
    3. 与 Bietti 晶体性角膜营养不良症相关的 CYP4V2 基因新突变。  |  Shan, M., et al. 2005. Mol Vis. 11: 738-43. PMID: 16179904
    4. CYP4V2突变的Bietti晶体营养不良症患者的特征。  |  Lee, KY., et al. 2005. Invest Ophthalmol Vis Sci. 46: 3812-6. PMID: 16186368
    5. 熟食诱变剂 2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）在 MCF10A 乳腺上皮细胞中的细胞色素 P450 表达和代谢活化。  |  Thomas, RD., et al. 2006. Chem Biol Interact. 160: 204-16. PMID: 16527260
    6. CYP4V2基因突变患者比蒂氏晶体营养不良症的基因型表型分析。  |  Lai, TY., et al. 2007. Invest Ophthalmol Vis Sci. 48: 5212-20. PMID: 17962476
    7. 一名墨西哥比埃蒂氏结晶性角膜营养不良症患者的新型 CYP4V2 基因突变。  |  Zenteno, JC., et al. 2008. Curr Eye Res. 33: 313-8. PMID: 18398705
    8. 共同暴露于 2,3,7,8-四氯二苯并对二恶英和全氟辛烷磺酸或全氟辛酸对鸡（Gallus gallus）胚胎肝细胞中细胞色素 P450 同工酶表达的影响。  |  Watanabe, MX., et al. 2009. Comp Biochem Physiol C Toxicol Pharmacol. 149: 605-12. PMID: 19167519

    订购信息

    产品名称产品编号规格价格数量收藏夹

    CYP4V2 抗体 (M29-P3B10)

    sc-101386
    200 µg/ml
    		RMB2377.00

    CYP4V2 (M29-P3B10): m-IgG Fc BP-HRP 套装

    sc-528718
    200 µg Ab; 10 µg BP
    		RMB2662.00

    CYP4V2 (M29-P3B10): m-IgGκ BP-HRP 套装

    sc-521189
    200 µg Ab, 40 µg BP
    		RMB2662.00

    CYP4V2 (M29-P3B10): m-IgG1 BP-HRP 套装

    sc-543088
    200 µg Ab; 20 µg BP
    		RMB2662.00

    CYP4V2 抗体 (M29-P3B10) AC

    sc-101386 AC
    500 µg/ml, 25% agarose
    		RMB3129.00

    CYP4V2 抗体 (M29-P3B10) HRP

    sc-101386 HRP
    200 µg/ml
    		RMB2377.00

    CYP4V2 抗体 (M29-P3B10) FITC

    sc-101386 FITC
    200 µg/ml
    		RMB2482.00

    CYP4V2 抗体 (M29-P3B10) PE

    sc-101386 PE
    200 µg/ml
    		RMB2580.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 488

    sc-101386 AF488
    200 µg/ml
    		RMB2685.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 546

    sc-101386 AF546
    200 µg/ml
    		RMB2685.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 594

    sc-101386 AF594
    200 µg/ml
    		RMB2685.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 647

    sc-101386 AF647
    200 µg/ml
    		RMB2685.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 680

    sc-101386 AF680
    200 µg/ml
    		RMB2685.00

    CYP4V2 抗体 (M29-P3B10) Alexa Fluor® 790

    sc-101386 AF790
    200 µg/ml
    		RMB2685.00

    CYP4V2蛋白预测大小为61KD，为什么你们做的人肝微粒体中的western显示大小不足45KD？

    Asked by: CYP4V2
    感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
    Answered by: Technical Service SH
    Date published: 2025-08-08

    请教个问题：CYP4V2 抗体 (M29-P3B10): sc-101386，该抗体可以检测小鼠的同源蛋白CYP4V3吗？

    Asked by: 那年天空
    感谢您的提问。CYP4V2 抗体 (M29-P3B10) 推荐用于WB, IP 和 ELISA，检测human 来源的 CYP4V2。对于小鼠CYP4V3蛋白，该抗体没有检测过，故不推荐。 Thank you for your question. CYP4V2 antibody (M29-P3B10) is recommended for detection of CYP4V2 of human origin by WB, IP and ELISA. We didn't test this antibody on mouse CYP4V3 protein, so it is not recommended for detection of mouse CYP4V3 protein.
    Answered by: Technical Service SH
    Date published: 2022-02-28

    What is the recommended starting dilution for Western blot for sc-101386: CYP4V2 (M29-P3B10) monoclonal antibody?

    Asked by: Trav11
    The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-22
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    Rated 4 out of 5 by from Can this antibody be used in ARPE-19 cell line?Can this antibody CYP4V2抗体(M29-P3B10): sc-101386 be used in ARPE-19 cell line?
    Date published: 2023-06-15
    Rated 5 out of 5 by from Worked very wellWorked very well at suggest dilution 1:1000. 1-2 hour incubated primary antibody,blocking use 5% BSA, no background.
    Date published: 2017-09-02
    Rated 5 out of 5 by from Recommended use.Used this antibody for WB detection and it worked well,recommended use.
    Date published: 2017-04-28
    Rated 5 out of 5 by from the result is expected.Band in the work found in a large number of cell lines, the result is expected.
    Date published: 2017-02-11
    Rated 5 out of 5 by from Powerful bandPowerful band detected in a large number of cell lines, the result satisfied.
    Date published: 2016-12-10
    Rated 4 out of 5 by from Good detection of CYP4V2 with human liverGood detection of CYP4V2 with human liver microsomes in WB -SCBT QC
    Date published: 2014-12-21
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    CYP4V2 抗体 (M29-P3B10) is rated 4.7 out of 5 by 6.
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