Date published: 2025-9-7
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CYP3A4 Antibody (HL3) - Immunohistochemistry - Image 370187
CYP3A4 抗体 (HL3): sc-53850. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
CYP3A4 抗体 (HL3): sc-53850. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

CYP3A4 抗体 (HL3): sc-53850

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说明书
  • CYP3A4 抗体 HL3 是小鼠单克隆 IgG1 κ，CYP3A4抗体, 在85篇文献中引用，规格为200 µg/ml
  • 针对部分纯化的肝微粒体（human起源）
  • CYP3A4 抗体 (HL3) 推荐用于 WB, IP, IF 和 IHC(P)，检测 CYP3A4 of human origin and the corresponding rat homolog
  • 抗CYP3A4抗体（HL3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP3A4 (HL3): sc-53850的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CYP3A4 抗体 (HL3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CYP3A4 抗体 (HL3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CYP 抗体 包括 CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1和CYP27B1

CYP3A4 抗体 (HL3) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组化等应用检测人和大鼠样本中的细胞色素 P450 3A4 (CYP3A4)。抗 CYP3A4 抗体 (HL3) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。CYP3A4 在药物代谢中起着至关重要的作用，因为 CYP3A4 负责超过 50% 的临床用药的生物转化，这使得 CYP3A4 成为药代动力学和个性化医疗的关键。CYP3A4 主要位于肝细胞和肠道上部肠细胞的内质网中，糖皮质激素和其他药剂可诱导 CYP3A4 的表达，从而显著影响药物的清除率和疗效。位于 7q22.1 位点的 CYP3A 基因簇包括 CYP3A4、CYP3A5、CYP3A7 和 CYP3A43 以及两个假基因，凸显了该酶家族在药物代谢和胆固醇、类固醇及其他脂类合成过程中的复杂性和重要性。了解 CYP3A4 的功能和调控对优化治疗策略和减少药物不良反应至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP3A4 抗体 (HL3) 参考文献:

  1. 体外评估药物对 CYP3A4 和其他 P-450 酶抑制作用的相关问题及其对药物发现的影响。  |  Wienkers, LC. 2001. J Pharmacol Toxicol Methods. 45: 79-84. PMID: 11489668
  2. 同时克服 P 糖蛋白介导的药物外流和 CYP3A4 介导的药物代谢的策略。  |  Patel, J. and Mitra, AK. 2001. Pharmacogenomics. 2: 401-15. PMID: 11722289
  3. 人类乳腺肿瘤和非肿瘤组织中 CYP3A4 的表达。  |  Kapucuoglu, N., et al. 2003. Cancer Lett. 202: 17-23. PMID: 14643022
  4. CYP3A4 对胆汁酸解毒的前馈调节：人源化转基因小鼠研究。  |  Stedman, C., et al. 2004. J Biol Chem. 279: 11336-43. PMID: 14681232
  5. 与甲孕酮和黄体酮结合的人类细胞色素 P450 3A4 的晶体结构。  |  Williams, PA., et al. 2004. Science. 305: 683-6. PMID: 15256616
  6. CYP3A4基因型与正在接受 ALL 化疗的儿科癌症患者外周血白细胞中 Vgamma/Jbeta 反式重组检测的关系。  |  Lopes, LF., et al. 2004. Leuk Res. 28: 1281-6. PMID: 15475069
  7. 结直肠癌细胞色素 p450 图谱：确定预后标志物。  |  Kumarakulasingham, M., et al. 2005. Clin Cancer Res. 11: 3758-65. PMID: 15897573
  8. CYP3A4与膳食多酚之间的相互作用。  |  Basheer, L. and Kerem, Z. 2015. Oxid Med Cell Longev. 2015: 854015. PMID: 26180597
  9. 用人类细胞色素 P-450 单克隆抗体对正常人肝脏和肝外组织中的细胞色素 P-450 进行免疫细胞化学定位和分布。  |  Murray, GI., et al. 1988. Br J Clin Pharmacol. 25: 465-75. PMID: 3289600
  10. CYP3A4和CYP2C9基因型对SWOG S0226中全身阿那曲唑和氟维司群浓度的影响。  |  Rutherford, DV., et al. 2023. Pharmacogenomics. 24: 665-673. PMID: 37615099
  11. 黄酮类化合物作为CYP3A4的体外抑制剂。  |  Kondža, M., et al. 2024. Biomedicines. 12: PMID: 38540257
  12. CYP3A4和CYP2C19基因多态性与杨梅素对托法替尼代谢的相互作用。  |  Ye, Z., et al. 2024. Biomed Pharmacother. 175: 116421. PMID: 38719708

订购信息

产品名称产品编号规格价格数量收藏夹

CYP3A4 抗体 (HL3)

sc-53850
200 µg/ml
RMB2377.00

CYP3A4 (HL3): m-IgG Fc BP-HRP 套装

sc-528580
200 µg Ab; 10 µg BP
RMB2662.00

CYP3A4 (HL3): m-IgGκ BP-HRP 套装

sc-520991
200 µg Ab, 40 µg BP
RMB2662.00

CYP3A4 (HL3): m-IgG1 BP-HRP 套装

sc-543007
200 µg Ab; 20 µg BP
RMB2662.00

CYP3A4 抗体 (HL3) AC

sc-53850 AC
500 µg/ml, 25% agarose
RMB3129.00

CYP3A4 抗体 (HL3) HRP

sc-53850 HRP
200 µg/ml
RMB2377.00

CYP3A4 抗体 (HL3) FITC

sc-53850 FITC
200 µg/ml
RMB2482.00

CYP3A4 抗体 (HL3) PE

sc-53850 PE
200 µg/ml
RMB2580.00

CYP3A4 抗体 (HL3) Alexa Fluor® 488

sc-53850 AF488
200 µg/ml
RMB2685.00

CYP3A4 抗体 (HL3) Alexa Fluor® 546

sc-53850 AF546
200 µg/ml
RMB2685.00

CYP3A4 抗体 (HL3) Alexa Fluor® 594

sc-53850 AF594
200 µg/ml
RMB2685.00

CYP3A4 抗体 (HL3) Alexa Fluor® 647

sc-53850 AF647
200 µg/ml
RMB2685.00

CYP3A4 抗体 (HL3) Alexa Fluor® 680

sc-53850 AF680
200 µg/ml
RMB2685.00

CYP3A4 抗体 (HL3) Alexa Fluor® 790

sc-53850 AF790
200 µg/ml
RMB2685.00

Hello, Can you provide carrier-free CYP3A4 antibodies? Also, could you share the epitope information of the antibody?

Asked by: Haojie Zhu
Thank you for your question. For further assistance please contact our special orders department for a price quote and ship date. Please email [email protected]. This antibody has not been epitope mapped but here is some information about the protien. https://www.uniprot.org/uniprotkb/P08684/entry
Answered by: BlakeJ
Date published: 2025-01-15

Is it possible to get a free trial size of this antibody to test on my sample? if so could you please elaborate the procedure!

Asked by: Harin
Contact Technical service about the current sample program.
Answered by: Tech Sales
Date published: 2021-07-09

How is the specifity of this CYP3A4 antibody? Does it detect CYP3A5 or CYP3A7? Thank you!

Asked by: Alex001
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Hello,I am working with HEPG2 cells, my bands are around 36 kDa. I think they are in the wrong place because there is no difference in different siRNA applications. I used odontoblast ( not expressed CYP3A4), but there is a band. Any recommendation?

Asked by: thak
Thank you for your question. Please contact your local distributor or technical service directly for assistance regarding your results, we are happy to help troubleshoot this with you.
Answered by: Tech Service 1
Date published: 2020-11-16

Can you recommend a positive control to use with CYP3A4 Antibody (HL3): sc-53850 for Western blot?

Asked by: TinTin
We recommend human liver tissue extract (sc-363766) to use as positive control as we have Western blot data showing specific detection of CYP3A4 in this sample.
Answered by: Technical Support 8
Date published: 2017-01-10
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Rated 5 out of 5 by from Excelente resultadoHe probado este anticuerpo en diferentes tecnicas (IHC, IF, WB, CF) y en todas tuve muy buenos resultados
Date published: 2019-05-22
Rated 4 out of 5 by from Good antibodyWorks as we expected. Tested by immunofluorescence
Date published: 2018-12-18
Rated 5 out of 5 by from Strong detection in Rat liver samples大鼠肝脏样本，表达量很高，条带很清晰。拿到说明书后发现优先使用人源样本，但是已证实大鼠样本也是可以的。
Date published: 2018-01-23
Rated 5 out of 5 by from anti-CYP3A4Good CYP3A4 detection by Western blot in a subcellular fraction enriched in intracellular membranes.
Date published: 2017-12-12
Rated 5 out of 5 by from specific signalI used this antibody detect CYP3A4 protein , it shows high specific signal was observed for CYP3A4 expressing in human liver tissue with no signal from negative control samples at dilution 1:600,the result is satisfied to our mind.
Date published: 2017-07-29
Rated 4 out of 5 by from western blotIn HepG2 cells, the signal is high and bands are clear, but the backgroung is also high at the dilution of 1:500, so we want to try again.
Date published: 2017-07-27
Rated 5 out of 5 by from Good detectionWe use CYP3A4 antibody to detect the expression by diluting 1:10000 in TBS-Tween for one hour, and there is enough an excellent western blot signal. Loading of 30 μg microsomal proteins is recommended for quantification.
Date published: 2017-04-03
Rated 5 out of 5 by from Clear WB resultsVery clean results in western blot studies; low background
Date published: 2017-01-11
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CYP3A4 抗体 (HL3) is rated 4.8 out of 5 by 14.
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