Date published: 2025-9-7
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CYP1A1 Antibody (A-9) - Western Blotting - Image 375647
CYP1A1 抗体 (A-9): sc-393979. HeLa (A), Neuro-2A (B), MCF7 (C), Hep G2 (D), SK-BR-3 (E) 和 EOC 20 (F) 全细胞裂解液中 CYP1A1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
CYP1A1 抗体 (A-9): sc-393979. HeLa (A), Neuro-2A (B), MCF7 (C), Hep G2 (D), SK-BR-3 (E) 和 EOC 20 (F) 全细胞裂解液中 CYP1A1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

CYP1A1 抗体 (A-9): sc-393979

4.3(8)
寫評論提問

说明书
  • CYP1A1 抗体 A-9 是小鼠单克隆 IgG1 κ，CYP1A1抗体, 在27篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的CYP1A1的C-terminus附近的氨基酸479-506之间
  • CYP1A1 抗体 (A-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CYP1A1
  • 抗CYP1A1抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP1A1 (A-9): sc-393979的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CYP1A1 Antibody (A-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CYP1A1 Antibody (A-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外CYP抗体，包括CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1, CYP27B1, CYP2A6, CYP2B1, CYP2C6, CYP2D6, CYP2F1和CYP2F2

CYP1A1抗体（A-9）是一种IgG1 κ小鼠单克隆CYP1A1抗体（也称为细胞色素P450 1A1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CYP1A1蛋白。CYP1A1抗体（A-9）既可以是非偶联抗CYP1A1抗体形式，也可以是多种偶联形式的抗CYP1A1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。P450酶构成单加氧酶酶家族，参与多种内源性和外源性化合物的代谢。几种P450酶已被分类为序列相似的CYP1A和CYP2A亚家族成员。NADPH细胞色素P450还原酶是一种微粒体酶，负责在P450催化循环中将电子从NADPH转移到细胞色素P450酶。NADPH细胞色素P450还原酶定位于内质网，在那里它还能够将电子转移到血红素加氧酶和细胞色素b5。NADPH细胞色素P450还原酶在结构上与两个不同的黄素蛋白家族、铁氧还蛋白核苷酸还原酶（FNR）和黄素蛋白有关。NADPH细胞色素P450还原酶的电子转移需要两个黄素辅因子FAD和FMN分别与FNR和黄素蛋白结构域结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CYP1A1 抗体 (A-9)

sc-393979
200 µg/ml
RMB2377.00

CYP1A1 (A-9): m-IgG Fc BP-HRP 套装

sc-530649
200 µg Ab; 10 µg BP
RMB2662.00

CYP1A1 (A-9): m-IgGκ BP-HRP 套装

sc-524165
200 µg Ab, 40 µg BP
RMB2662.00

CYP1A1 (A-9): m-IgG1 BP-HRP 套装

sc-544178
200 µg Ab; 20 µg BP
RMB2662.00

CYP1A1 抗体 (A-9) AC

sc-393979 AC
500 µg/ml, 25% agarose
RMB3129.00

CYP1A1 抗体 (A-9) HRP

sc-393979 HRP
200 µg/ml
RMB2377.00

CYP1A1 抗体 (A-9) FITC

sc-393979 FITC
200 µg/ml
RMB2482.00

CYP1A1 抗体 (A-9) PE

sc-393979 PE
200 µg/ml
RMB2580.00

CYP1A1 抗体 (A-9) Alexa Fluor® 488

sc-393979 AF488
200 µg/ml
RMB2685.00

CYP1A1 抗体 (A-9) Alexa Fluor® 546

sc-393979 AF546
200 µg/ml
RMB2685.00

CYP1A1 抗体 (A-9) Alexa Fluor® 594

sc-393979 AF594
200 µg/ml
RMB2685.00

CYP1A1 抗体 (A-9) Alexa Fluor® 647

sc-393979 AF647
200 µg/ml
RMB2685.00

CYP1A1 抗体 (A-9) Alexa Fluor® 680

sc-393979 AF680
200 µg/ml
RMB2685.00

CYP1A1 抗体 (A-9) Alexa Fluor® 790

sc-393979 AF790
200 µg/ml
RMB2685.00

CYP1A1 (A-9) 中和勝肽

sc-393979 P
100 µg/0.5 ml
RMB511.00

HI TECHNICAL SUPPORT, CYP1A1 (A-9) in my phd progect. i found clear band for mda-mb468 on nitrocelluse membrane at dilution 1:100 incubation over night - i could not detect band in MCF-7 and mda-231 could you help me please as i want to purchas

Asked by: deem
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-05-20

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-13

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 12
Date published: 2017-01-13
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Rated 4 out of 5 by from Great for WBWorks well for western blot in hRECs and human RCC cells.
Date published: 2022-06-08
Rated 4 out of 5 by from Worked well for human and mouse samplesWorked well when using for WB at 1:200 dilution. Provided nice clear bands in both human and mouse tissue lysates after longer exposure times.
Date published: 2018-03-22
Rated 1 out of 5 by from Not really working.. :-(Tried in WB of human hepatocytes Huh7 and Huh7.5. No bands at the expected size, only some faint bands at the bottom of the blot (maybe 25-30kDa).
Date published: 2018-01-22
Rated 5 out of 5 by from Good for WB1:200 diluted for wb of rat heart protein,there is a clear band at the position above 70KD.
Date published: 2017-10-20
Rated 5 out of 5 by from Nice Western BlotNice Western Blot data of CYP1A1 expression in HCC whole cell lysates.
Date published: 2017-05-03
Rated 5 out of 5 by from Excellent new antibody!There are several good CYP1A1 clones to choose from, but this new CYP1A1 (A-9) clone gives excellent results in our in-house testing by Western blotting in mouse, rat and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from GreatGreat. It appeared as a single band at the size expected. No background bands found. I used HCC827 Lung Cancer Cells for western blot.
Date published: 2015-03-26
Rated 5 out of 5 by from Great single bands in WB with HeLa and NeuroGreat single bands in WB with HeLa and Neuro-2A whole cell lysates -SCBT QC
Date published: 2014-07-13
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CYP1A1 抗体 (A-9) is rated 4.3 out of 5 by 8.
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