Date published: 2025-9-7
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CYP17A1 Antibody (D-12) - Immunohistochemistry - Image 350189
CYP17A1 抗体 (D-12): sc-374244. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
CYP17A1 抗体 (D-12): sc-374244. 福尔马林固定, 石蜡包埋的人肾上腺组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.

CYP17A1 抗体 (D-12): sc-374244

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说明书
  • CYP17A1 抗体 D-12 是小鼠单克隆 IgG2a κ，CYP17A1抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列461-508位于human物种的CYP17A1的C-terminus
  • CYP17A1 抗体 (D-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 CYP17A1
  • 抗CYP17A1抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CYP17A1 (D-12): sc-374244的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 CYP17A1 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CYP17A1 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 CYP 抗体 包括 CYP11B, CYP17A1, CYP19, CYP1A1, CYP1A2, CYP1B1, CYP24, CYP26A1, CYP27A1和CYP27B1

CYP17A1抗体（D-12）是一种IgG2a κ小鼠单克隆CYP17A1抗体（也称为CP17AC抗体、细胞色素P450家族17亚家族A成员1抗体、类固醇17-α-单加氧酶抗体、S17AH抗体、细胞色素P450家族17亚家族A多肽1抗体、17-α-羟基孕酮醛缩酶抗体、CYPXVII抗体、CYP17抗体或CPT7抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源CYP17A1蛋白。CYP17A1抗体（D-12）既可作为非共轭抗CYP17A1抗体形式，也可作为多种共轭形式的抗CYP17A1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。细胞色素P450蛋白是单加氧酶，可催化药物代谢以及胆固醇、类固醇和其他脂质的合成所涉及的许多反应。基于序列相似性，P450酶被分为不同的亚家族，如CYP1A、CYP2A、CYP2C、CYP2D、CYP4A14、CYP7A、CYP7B、CYP8B、CYP11A、CYP17A1、CYPI9和CYP27A。CYP17A（17α-羟化酶/17,20-裂解酶）对于将孕烯醇酮和孕酮转化为脱氢表雄酮（DHEA）和雄烯二酮非常重要。在此过程中，它催化17-α-羟基化和17,20-裂解反应。CYP17A1在胎儿发育和青春期期间的性发育过程中至关重要，并且受细胞内cAMP水平的调节。编码该蛋白的CYP17A1基因缺陷可能导致肾上腺增生症V型（AH-V），其特征为低钾血症和高血压。受AH-V影响的男性患者不会经历正常的性别分化，并会发育出女性外生殖器，并且不会经历青春期发育。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CYP17A1 抗体 (D-12) 参考文献:

  1. 以 17α- 羟化酶/17,20-赖氨酸酶缺乏症为模型研究酶活性调节：磷酸化的作用。  |  Biason-Lauber, A., et al. 2000. J Clin Endocrinol Metab. 85: 1226-31. PMID: 10720067
  2. 巴西患者的 P450c17 缺乏症：通过 CYP17 基因分型确认孕酮水平的生化诊断。  |  Martin, RM., et al. 2003. J Clin Endocrinol Metab. 88: 5739-46. PMID: 14671162
  3. 表面上孤立的 17,20-lyase 缺乏症的分子基础：C-末端区域的复合杂合突变（Arg(496)----Cys, Gln(461)----Stop）实际上导致 17 alpha-hydroxylase/17,20-lyase 合并缺乏症。  |  Yanase, T., et al. 1992. Biochim Biophys Acta. 1139: 275-9. PMID: 1515452
  4. 细胞色素 P450 氧化还原酶基因突变与伴有生殖器异常和/或类固醇生成障碍的安特雷-比克斯勒综合征：对 10 名患者进行的分子和临床研究。  |  Fukami, M., et al. 2005. J Clin Endocrinol Metab. 90: 414-26. PMID: 15483095
  5. 通过芯片分析确定的新生小鼠睾丸中的雄激素调控转录本。  |  Zhou, Q., et al. 2005. Biol Reprod. 72: 1010-9. PMID: 15601916
  6. 习惯性吸烟和饮酒与 40 个候选基因中单核苷酸多态性 (SNP) 的关联：日本随机人群样本数据。  |  Liu, Y., et al. 2005. J Hum Genet. 50: 62-68. PMID: 15654505
  7. CYP17（P45017 alpha）基因中的复合杂合突变（Arg 239----stop，Pro 342----Thr）导致一名部分合并17α-羟化酶/17,20-赖氨酸酶缺乏症的男性患者出现外生殖器畸形。  |  Ahlgren, R., et al. 1992. J Clin Endocrinol Metab. 74: 667-72. PMID: 1740503
  8. 去势抵抗性前列腺癌中的CYP17A1抑制剂。  |  Gomez, L., et al. 2015. Steroids. 95: 80-7. PMID: 25560485
  9. CYP17A1突变可促进动脉粥样硬化和早发性冠状动脉疾病。  |  Wu, TT., et al. 2023. Cell Commun Signal. 21: 155. PMID: 37370070
  10. 阿比特龙（abiraterone）羟肟酸对CYP17A1和HDAC6的双重抑制作用可通过诱导DNA损伤和氧化应激来克服胶质母细胞瘤对替莫唑胺的耐药性。  |  Sharma, R., et al. 2024. Cancer Lett. 586: 216666. PMID: 38311053
  11. 细胞色素 P450c17 中 Arg440 到 His 的点突变会导致严重的 17 alpha-hydroxylase 缺乏症。  |  Fardella, CE., et al. 1994. J Clin Endocrinol Metab. 79: 160-4. PMID: 8027220
  12. 细胞色素 P450c17 中组氨酸 373 突变为亮氨酸会导致 17 α-羟化酶缺乏症。  |  Monno, S., et al. 1993. J Biol Chem. 268: 25811-7. PMID: 8245018

订购信息

产品名称产品编号规格价格数量收藏夹

CYP17A1 抗体 (D-12)

sc-374244
200 µg/ml
RMB2377.00

CYP17A1 (D-12): m-IgG Fc BP-HRP 套装

sc-525945
200 µg Ab; 10 µg BP
RMB2662.00

CYP17A1 (D-12): m-IgG2a BP-HRP 套装

sc-546466
200 µg Ab; 10 µg BP
RMB2662.00

CYP17A1 抗体 (D-12) AC

sc-374244 AC
500 µg/ml, 25% agarose
RMB3129.00

CYP17A1 抗体 (D-12) HRP

sc-374244 HRP
200 µg/ml
RMB2377.00

CYP17A1 抗体 (D-12) FITC

sc-374244 FITC
200 µg/ml
RMB2482.00

CYP17A1 抗体 (D-12) PE

sc-374244 PE
200 µg/ml
RMB2580.00

CYP17A1 抗体 (D-12) Alexa Fluor® 488

sc-374244 AF488
200 µg/ml
RMB2685.00

CYP17A1 抗体 (D-12) Alexa Fluor® 546

sc-374244 AF546
200 µg/ml
RMB2685.00

CYP17A1 抗体 (D-12) Alexa Fluor® 594

sc-374244 AF594
200 µg/ml
RMB2685.00

CYP17A1 抗体 (D-12) Alexa Fluor® 647

sc-374244 AF647
200 µg/ml
RMB2685.00

CYP17A1 抗体 (D-12) Alexa Fluor® 680

sc-374244 AF680
200 µg/ml
RMB2685.00

CYP17A1 抗体 (D-12) Alexa Fluor® 790

sc-374244 AF790
200 µg/ml
RMB2685.00

I am using CYP17A1 (D-12): sc-374244 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-23
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Rated 4 out of 5 by from Works very well for WB in the mouse testisThe good: We have tried every cyp17a1 antibody we could find (from multiple companies) to identify the best one for WB, IF, and IHC. This antibody is by far the best for WB for use in the mouse testis at 1:250 dilution. There are two non-specific bands, but they are very obviously the wrong size. All other antibodies we tried for WB gave many more non-specific bands. We have tried three different lots of this antibody and they have all worked similarly! The not so good: 1) This antibody did not work well for IF/IHC in the mouse testis using our standard protocol. There was a lot of non-specific binding in cell types that do not express this enzyme. Perhaps with more optimization on our end this could be improved. 2) This antibody seems to lose potency within a few months despite being stored as directed, and we have to re-order way before we have used the entire amount. Its still worth it though, for WB.
Date published: 2019-08-30
Rated 5 out of 5 by from Great for WBThe CYP17A1 Antibody (D-12): sc-374244 works very well for Western blot applications, showing strong and clear band with minimal background.
Date published: 2017-02-08
Rated 1 out of 5 by from Ne fonctionne pas chez la sourisNe fonctionne ni en WB sur des extraits de testicules de souris ni en immunohistochimie sur des testicules de souris
Date published: 2017-02-02
Rated 5 out of 5 by from Worked great with porcine samples!I received a sample of this antibody for use in western blotting. It works great with porcine testis samples! It gave one beautiful band of an appropriate molecular weight. It also demonstrated linearity with increasing protein concentrations and the negative control was clear. We are very happy.
Date published: 2016-11-30
Rated 5 out of 5 by from work with pig testies leydig cellswork with pig testies leydig cells
Date published: 2016-08-08
Rated 5 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2014-08-25
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CYP17A1 抗体 (D-12) is rated 4.2 out of 5 by 6.
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