Date published: 2025-9-7

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cyclin E 抗体 (HE111): sc-248

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说明书
  • cyclin E抗体(HE111)是小鼠单克隆IgG1 λ, 在134篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组cyclin E
  • 抗-cyclin E 抗体 (HE111) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 cyclin E
  • 抗cyclin E抗体(HE111)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-248 X, 200 µg/0.1 ml)
  • 关于如何获取cyclin E (HE111): sc-248免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGλ BP-HRP 是 cyclin E 抗体 (HE111) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cyclin E 抗体 (HE111) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

细胞周期蛋白E抗体(HE111)是一种小鼠单克隆IgG1 lambda轻链抗体,可通过蛋白质印迹、免疫沉淀、免疫荧光和免疫组织化学检测小鼠、大鼠和人类来源的细胞周期蛋白E。Cyclin E (HE111) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。Cyclin E 在细胞周期的调节中起着关键作用,特别是在从 G1 期到 S 期的过渡期间。细胞周期蛋白E与转录因子E2F结合,促进DNA合成所需基因的表达。细胞周期蛋白E/E2F复合物在G1期最为活跃,细胞周期蛋白E促进S期特异性蛋白的表达,确保细胞为DNA复制做好充分准备。细胞周期蛋白E水平的精确调控至关重要,因为失调会导致细胞无序增殖,并常常与多种癌症有关。Cyclin E 单克隆抗体(HE111)是研究细胞周期动力学以及细胞分裂和癌症生物学分子机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin E 抗体 (HE111)

sc-248
200 µg/ml
RMB2377.00

cyclin E (HE111): m-IgG Fc BP-HRP 套装

sc-551158
200 µg Ab; 10 µg BP
RMB2662.00

cyclin E (HE111): m-IgGλ BP-HRP 套装

sc-551157
200 µg Ab; 40 µg BP
RMB2662.00

cyclin E (HE111): m-IgG1 BP-HRP 套装

sc-551159
200 µg Ab; 20 µg BP
RMB2662.00

cyclin E 抗体 (HE111) X

sc-248 X
200 µg/0.1 ml
RMB2377.00

cyclin E 抗体 (HE111) AC

sc-248 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin E 抗体 (HE111) HRP

sc-248 HRP
200 µg/ml
RMB2377.00

cyclin E 抗体 (HE111) FITC

sc-248 FITC
200 µg/ml
RMB2482.00

cyclin E 抗体 (HE111) PE

sc-248 PE
200 µg/ml
RMB2580.00

cyclin E 抗体 (HE111) Alexa Fluor® 488

sc-248 AF488
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) Alexa Fluor® 546

sc-248 AF546
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) Alexa Fluor® 594

sc-248 AF594
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) Alexa Fluor® 647

sc-248 AF647
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) Alexa Fluor® 680

sc-248 AF680
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) Alexa Fluor® 790

sc-248 AF790
200 µg/ml
RMB2685.00

cyclin E 抗体 (HE111) TRITC

sc-248 TRITC
200 µg/ml
RMB2407.00

Why is CCNE1detecting a 90kD band with this antibody?

Asked by: Ling
Thank you for your question. The protein undergoes phosphorylation.https://www.uniprot.org/uniprotkb/P24864/entry#ptm_processing
Answered by: BlakeJ
Date published: 2024-08-09

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from mouse livernice antibody 1:500 dilution, in mouse liver tissue.
Date published: 2017-03-31
Rated 5 out of 5 by from Great IHC resultsTested multiple samples by IHC in paraffin embedded sections with great results!
Date published: 2017-01-17
Rated 5 out of 5 by from Good data with paraffinGood data with paraffin-embedded human ovary tissue. -SCBT Publication Review
Date published: 2015-02-11
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for IP with MEG-01 samples. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from WB: Great antibodyWB: Great antibody, good images for publication with human U2OS. -SCBT Publication Review
Date published: 2014-08-20
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cyclin E 抗体 (HE111) is rated 4.8 out of 5 by 6.
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