Date published: 2025-9-7
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cyclin B2 Antibody (A-2) - Western Blotting - Image 6648
细胞周期蛋白 B2 抗体 (A-2): sc-28303. K-562 (A) 和 F9 (B) 全细胞裂解液中细胞周期蛋白 B2 表达的 Western 印迹分析.
细胞周期蛋白 B2 抗体 (A-2): sc-28303. K-562 (A) 和 F9 (B) 全细胞裂解液中细胞周期蛋白 B2 表达的 Western 印迹分析.

cyclin B2 抗体 (A-2): sc-28303

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寫評論提問

说明书
  • cyclin B2 抗体 A-2 是小鼠单克隆 IgG1 κ，cyclin B2抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫human物种的cyclin B2的氨基酸1-105
  • cyclin B2 抗体 (A-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 cyclin B2
  • 抗cyclin B2抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cyclin B2 (A-2): sc-28303的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是cyclin B2 Antibody (A-2) for WB applications. 的首选辅助检测试剂，这些试剂现与cyclin B2 Antibody (A-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Cyclin抗体，包括cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2, cyclin D3, cyclin E, cyclin E2, cyclin F, cyclin G1, cyclin G2和cyclin H

cyclin B2 抗体（A-2）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 cyclin B2 蛋白。anti-cyclin B2 抗体（A-2）有非结合型和多种结合型，包括琼脂糖 、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。细胞周期蛋白B2在调节细胞周期中起着至关重要的作用，特别是在从G2期到有丝分裂的过渡期间。细胞周期蛋白B2通过与细胞周期依赖性激酶Cdc2形成复合物发挥作用，而后者对于激活成熟促进因子（MPF）至关重要。这种激活对于有丝分裂的正常进行至关重要，可触发各种底物的磷酸化，从而引起染色质浓缩、核膜破裂和纺锤体的形成。对细胞周期蛋白B2和Cdc2相互作用进行精确调控至关重要，因为失调会导致细胞分裂失控，并常常与癌症有关。细胞周期蛋白B2单克隆抗体（A-2）是研究人员研究细胞周期动力学和有丝分裂调控的分子机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin B2 抗体 (A-2) 参考文献:

  1. 爪蟾卵母细胞中的细胞周期蛋白 B：对前蛋白激酶激活机制的影响。  |  Gautier, J. and Maller, JL. 1991. EMBO J. 10: 177-82. PMID: 1824935
  2. B 型细胞周期蛋白对 cdc25 酪氨酸磷酸酶的特异性激活：有丝分裂细胞周期蛋白多种作用的证据。  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
  3. Cyclin 是爪蟾成熟促进因子的一种成分。  |  Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
  4. 成熟促进因子对 M 期的控制  |  Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
  5. 用磷酸酶直接激活 cdc2：识别 p13suc1 敏感和不敏感步骤。  |  Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
  6. cdc2 的可逆酪氨酸磷酸化：进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  7. 多米诺骨牌与时钟：细胞周期两种观点的结合。  |  Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
  8. 细胞周期蛋白B2/CDK1复合物抑制小鼠卵母细胞减数分裂I期的分离酶活性。  |  Li, J., et al. 2019. Development. 146: PMID: 31704793
  9. Cyclin B2/CDK1复合物保守地抑制卵母细胞减数分裂II中的分离酶活性。  |  Li, J., et al. 2021. Front Cell Dev Biol. 9: 648053. PMID: 33777955
  10. Mad2促进细胞周期蛋白B2聚集到动点，从而引导精确的细胞分裂。  |  Liu, S., et al. 2022. EMBO Rep. 23: e54171. PMID: 35384228
  11. 细胞外囊泡衍生的 CircWhsc1 通过激活 TRIM59/STAT3/Cyclin B2 通路促进心肌细胞增殖和心脏修复。  |  Wei, G., et al. 2022. J Adv Res.. PMID: 36587763
  12. 小鼠细胞周期蛋白 B2 cDNA 的分离以及 B1 和 B2 细胞周期蛋白在卵子发生, 精子发生和早期胚胎发生过程中的表达谱系和时间特异性的鉴定。  |  Chapman, DL. and Wolgemuth, DJ. 1993. Development. 118: 229-40. PMID: 8375336

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin B2 抗体 (A-2)

sc-28303
200 µg/ml
RMB2377.00

cyclin B2 (A-2): m-IgG Fc BP-HRP 套装

sc-526658
200 µg Ab; 10 µg BP
RMB2662.00

cyclin B2 (A-2): m-IgG1 BP-HRP 套装

sc-532031
200 µg Ab; 20 µg BP
RMB2662.00

cyclin B2 抗体 (A-2) AC

sc-28303 AC
500 µg/ml, 25% agarose
RMB3129.00

cyclin B2 抗体 (A-2) HRP

sc-28303 HRP
200 µg/ml
RMB2377.00

cyclin B2 抗体 (A-2) FITC

sc-28303 FITC
200 µg/ml
RMB2482.00

cyclin B2 抗体 (A-2) PE

sc-28303 PE
200 µg/ml
RMB2580.00

cyclin B2 抗体 (A-2) Alexa Fluor® 488

sc-28303 AF488
200 µg/ml
RMB2685.00

cyclin B2 抗体 (A-2) Alexa Fluor® 546

sc-28303 AF546
200 µg/ml
RMB2685.00

cyclin B2 抗体 (A-2) Alexa Fluor® 594

sc-28303 AF594
200 µg/ml
RMB2685.00

cyclin B2 抗体 (A-2) Alexa Fluor® 647

sc-28303 AF647
200 µg/ml
RMB2685.00

cyclin B2 抗体 (A-2) Alexa Fluor® 680

sc-28303 AF680
200 µg/ml
RMB2685.00

cyclin B2 抗体 (A-2) Alexa Fluor® 790

sc-28303 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-28303: cyclin B2 (A-2) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-28303 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Just works for WBLoading amount: 10 ug of human MPNST (ST8814) cells Substrate: SuperSignal™ West Femto Maximum Sensitivity Substrate Dilution: 1:200 This antibody only worked with the most sensitive substrate.
Date published: 2019-11-26
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysatesTested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Recommend using CcnB1 antibody sc-7393 instead
Date published: 2019-05-22
Rated 5 out of 5 by from Works perfectly by WB on mouse colon extractsI tried this antobody on mouse colon protein extracts at a 1/200 dilution with around 20 ug of protein quantity. It showed a very nice signal at the right molecular weight
Date published: 2017-10-27
Rated 5 out of 5 by from 抗体效果不错今年年初，申请了该抗体的单克隆试用装，效果不错。组织标本做的WB，条带的特异性很好，首次使用，背景不高，但不可回收再利用。
Date published: 2017-09-25
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 4 out of 5 by from not suitable for turbotthere's no band in turbot. I used 1:100 and 1:2000 for second antibody ,exposed 10mns, no band
Date published: 2017-06-06
Rated 4 out of 5 by from 没法在大菱鲆上使用1:100用于大菱鲆细胞，曝了很长时间才有很浅很浅的带，杂带也较多，不太适合于大菱鲆。但是在3T3细胞较好，条带清晰
Date published: 2017-06-05
Rated 5 out of 5 by from very good for WBWe are very satisfied with cyclin B2 is really good and I have used it on my recent research, very good WB, signal strong at 1:500 dilution.
Date published: 2017-05-28
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cyclin B2 抗体 (A-2) is rated 4.4 out of 5 by 14.
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