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CCNB1/cyclin B1 Antibody (V152) - Western Blotting - Image 13575
cyclin B1 抗体 (V152): sc-53236. HeLa (A), PMA 处理的 HeLa (B), K-562 (C), PMA 处理的 K-562 (D), Jurkat (E) 和 PMA 处理的 Jurkat (F) 核提取物中细胞周期蛋白 B1 表达的 Western 印迹分析.
cyclin B1 抗体 (V152): sc-53236. HeLa (A), PMA 处理的 HeLa (B), K-562 (C), PMA 处理的 K-562 (D), Jurkat (E) 和 PMA 处理的 Jurkat (F) 核提取物中细胞周期蛋白 B1 表达的 Western 印迹分析.

CCNB1/cyclin B1 抗体 (V152): sc-53236

4.5(4)
寫評論提問

说明书
  • CCNB1/cyclin B1抗体(V152)是小鼠单克隆IgG1 κ, 在11篇文献中引用，规格为200 µg/ml
  • 针对His标签重组蛋白hamster cyclin B1的
  • 推荐用于 mouse, rat, human 和 hamster 来源的cyclin B1 WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CCNB1/cyclin B1 (GNS1): sc-245，可查看有标记的 CCNB1/cyclin B1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CCNB1/cyclin B1 抗体 (V152) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CCNB1/cyclin B1 抗体 (V152) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cyclin抗体，包括cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2, cyclin D3, cyclin E, cyclin E2, cyclin F, cyclin G1, cyclin G2和cyclin H

在真核细胞中，有丝分裂是由一种被称为促成熟因子、M期特异性组蛋白激酶或M期激酶的蛋白激酶激活后开始的。这种蛋白激酶由催化亚基（Cdc2）、调节亚基（cyclin B）和低分子量亚基（p13-Suc 1）组成。Cdc/cyclin酶受到多种水平的控制，其中由酪氨酸磷酸化调节催化亚基的调控是最好理解的。酪氨酸磷酸化抑制Cdc2/cyclin B酶；在细胞分裂开始时发生的酪氨酸去磷酸化直接激活了预MPF复合物。证据表明，B型cyclin不仅对M期调节亚基的Cdc2蛋白激酶有作用，而且还激活内源性酪氨酸磷酸酶Cdc25A和Cdc25B，其中Cdc2是生理底物。这种效应的特异性表现为cyclin A或cyclin D1无法显示任何对Cdc25A或Cdc25B的刺激。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CCNB1/cyclin B1 抗体 (V152)

sc-53236
200 µg/ml
RMB2377.00

CCNB1/cyclin B1 (V152): m-IgG Fc BP-HRP 套装

sc-536913
200 µg Ab; 10 µg BP
RMB2662.00

CCNB1/cyclin B1 (V152): m-IgGκ BP-HRP 套装

sc-534086
200 µg Ab; 40 µg BP
RMB2662.00

CCNB1/cyclin B1 (V152): m-IgG1 BP-HRP 套装

sc-544894
200 µg Ab; 20 µg BP
RMB2662.00

CCNB1/cyclin B1 抗体 (V152) FITC

sc-53236 FITC
200 µg/ml
RMB2482.00

CCNB1/cyclin B1 抗体 (V152) PE

sc-53236 PE
200 µg/ml
RMB2580.00

Can cyclin B1 (V152): sc-53236 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin B1 (V152): sc-53236 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect experimentThis antibody is great, specific, clean and strong results by WB
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody for IFGood antibody for IF staining in human colon tissue
Date published: 2017-04-18
Rated 4 out of 5 by from Reasonably specific and sensitiveReasonably specific and sensitive, good results in WB with HeLa, PMA treated HeLa, K-562, PMA treated K-562, Jurkat and PMA treated Jurkat nuclear extracts. -SCBT QC
Date published: 2015-03-19
Rated 4 out of 5 by from Good antibody for WesternGood antibody for Western, publication with epithelial ovarian cancer cell lines OVCA420, OVCA432, OVCA433, SKOV3, and HEY. -SCBT Publication Review
Date published: 2014-12-14
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CCNB1/cyclin B1 抗体 (V152) is rated 4.5 out of 5 by 4.
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