Date published: 2025-9-7
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CCNB1/cyclin B1 Antibody (GNS1) - Immunofluorescence - Image 319436
cyclin B1 抗体 (GNS1): sc-245. 福尔马林固定的 HeLa 细胞免疫荧光染色, 显示核定位. 山羊抗小鼠 IgG 与 CruzFluor® 647 succinimidyl ester 结合: sc-362620. 由波士顿儿童医院新生儿科, 哈佛医学院细胞生物学系向阳博士友情提供. 所用检测试剂: 山羊抗小鼠 IgG-CFL 647: sc-362287.
cyclin B1 抗体 (GNS1): sc-245. 福尔马林固定的 HeLa 细胞免疫荧光染色, 显示核定位. 山羊抗小鼠 IgG 与 CruzFluor® 647 succinimidyl ester 结合: sc-362620. 由波士顿儿童医院新生儿科, 哈佛医学院细胞生物学系向阳博士友情提供. 所用检测试剂: 山羊抗小鼠 IgG-CFL 647: sc-362287.

CCNB1/cyclin B1 抗体 (GNS1): sc-245

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说明书
  • CCNB1/cyclin B1 抗体 GNS1 是小鼠单克隆 IgG1 κ，CCNB1/cyclin B1抗体, 在1,250篇文献中引用，规格为200 µg/ml
  • 针对human的cyclin B1的重组蛋白
  • CCNB1/cyclin B1 抗体 (GNS1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 cyclin B1
  • 抗CCNB1/cyclin B1抗体（GNS1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-245 X, 200 µg/0.1 ml)
  • 关于如何获取CCNB1/cyclin B1 (GNS1): sc-245的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CCNB1/cyclin B1 抗体 (GNS1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CCNB1/cyclin B1 抗体 (GNS1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cyclin 抗体 包括 cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2和cyclin D3

cyclin B1抗体（GNS1）（也称为CCNB1抗体）是一种IgG1κ小鼠单克隆细胞周期蛋白B1抗体，可通过WB、IP、IF和IHC(P)检测小鼠、大鼠和人源的细胞周期蛋白B1。cyclin B1抗体（GNS1）既可以是非偶联的抗细胞周期蛋白B1抗体，也可以是多种偶联形式的抗细胞周期蛋白B1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核细胞中，有丝分裂是在一种蛋白激酶激活后启动的，这种蛋白激酶被称为成熟促进因子、M期特异性组蛋白激酶或M期激酶。该蛋白激酶由催化亚基（Cdc2）、调节亚基（细胞周期蛋白B）和低分子量亚基（p13-Suc 1）组成。Cdc/细胞周期蛋白酶受到多个层次的控制，其中通过酪氨酸磷酸化对催化亚基的调节是最好理解的。酪氨酸磷酸化抑制Cdc2/细胞周期蛋白B酶；酪氨酸去磷酸化发生在有丝分裂开始时，直接激活预MPF复合物。有证据表明，B型细胞周期蛋白不仅作用于Cdc2蛋白激酶的M期调节亚基，而且还激活Cdc25A和Cdc25B内源性酪氨酸磷酸酶，其中Cdc2是生理底物。这种效应的特异性表现在细胞周期蛋白A或细胞周期蛋白D1均无法显示对Cdc25A或Cdc25B的任何此类刺激。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CCNB1/cyclin B1 抗体 (GNS1) 参考文献:

  1. MPF 的摆动伴随着 cdc25 和 cdc2-cyclin B 之间的周期性结合。  |  Jessus, C. and Beach, D. 1992. Cell. 68: 323-32. PMID: 1310257
  2. 细胞色素B1和其他细胞色素作为肿瘤抗原在癌症免疫监测和免疫治疗中的作用。  |  Egloff, AM., et al. 2006. Cancer Res. 66: 6-9. PMID: 16397206
  3. 细胞周期蛋白 B1 通过 p38 MAPK 信号参与亚砷酸盐引发的 G2 检查点。  |  Li, JP. and Yang, JL. 2007. J Cell Physiol. 212: 481-8. PMID: 17373649
  4. 爪蟾卵母细胞中的细胞周期蛋白 B：对前蛋白激酶激活机制的影响。  |  Gautier, J. and Maller, JL. 1991. EMBO J. 10: 177-82. PMID: 1824935
  5. B 型细胞周期蛋白对 cdc25 酪氨酸磷酸酶的特异性激活：有丝分裂细胞周期蛋白多种作用的证据。  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
  6. Cyclin 是爪蟾成熟促进因子的一种成分。  |  Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
  7. 成熟促进因子对 M 期的控制  |  Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
  8. 用磷酸酶直接激活 cdc2：识别 p13suc1 敏感和不敏感步骤。  |  Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
  9. cdc2 的可逆酪氨酸磷酸化：进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  10. 多米诺骨牌与时钟：细胞周期两种观点的结合。  |  Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
  11. 细胞周期进展和肿瘤抗性中细胞色素B1/CDK1调节的线粒体生物能。  |  Xie, B., et al. 2019. Cancer Lett. 443: 56-66. PMID: 30481564
  12. 细胞周期蛋白 B1-Cdk1 的核-细胞质分区促进了有丝分裂事件的稳健定时。  |  Maryu, G. and Yang, Q. 2022. Cell Rep. 41: 111870. PMID: 36577372

订购信息

产品名称产品编号规格价格数量收藏夹

CCNB1/cyclin B1 抗体 (GNS1)

sc-245
200 µg/ml
RMB2377.00

CCNB1/cyclin B1 (GNS1): m-IgG Fc BP-HRP 套装

sc-528102
200 µg Ab; 10 µg BP
RMB2662.00

CCNB1/cyclin B1 (GNS1): m-IgGκ BP-HRP 套装

sc-520400
200 µg Ab, 40 µg BP
RMB2662.00

CCNB1/cyclin B1 (GNS1): m-IgG1 BP-HRP 套装

sc-542760
200 µg Ab; 20 µg BP
RMB2662.00

CCNB1/cyclin B1 抗体 (GNS1) X

sc-245 X
200 µg/0.1 ml
RMB2377.00

CCNB1/cyclin B1 抗体 (GNS1) AC

sc-245 AC
500 µg/ml, 25% agarose
RMB3129.00

CCNB1/cyclin B1 抗体 (GNS1) HRP

sc-245 HRP
200 µg/ml
RMB2377.00

CCNB1/cyclin B1 抗体 (GNS1) FITC

sc-245 FITC
200 µg/ml
RMB2482.00

CCNB1/cyclin B1 抗体 (GNS1) PE

sc-245 PE
200 µg/ml
RMB2580.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 488

sc-245 AF488
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 546

sc-245 AF546
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 594

sc-245 AF594
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 647

sc-245 AF647
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 680

sc-245 AF680
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) Alexa Fluor® 790

sc-245 AF790
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (GNS1) TRITC

sc-245 TRITC
200 µg/ml
RMB2407.00

Hi, do you already know if this antibody will react with bovine cells/tissue?

Asked by: clvleal
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi, do you know if the epitope locates on the N-terminal region or the C-terminal region? Thanks!

Asked by: NancySL
Thank you for your questions. Anti-cyclin B1 Antibody clone GNS1 has been reported to recognize an epitope in the N-terminus region of human cyclin B1.
Answered by: Santa Cruz TS
Date published: 2021-07-05

Will this antibody react with bovine protein? Than you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in bovine ABAE cells. Please refer to this reference on NCBI's PubMed, PMID: 15746189.
Answered by: Technical Support
Date published: 2017-08-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-11-03

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 5 out of 5 by from An optimal antibodyThe antibody anti-Cyclin B1 moAb (sc-245) demonstrated to work very well for WB, IF and IP
Date published: 2023-06-07
Rated 3 out of 5 by from Good for IF but not for WBThis antibody works well for IF at 1:100. However, it doesn't work for WB. I have tested it loading 20ug total lysate and got no band at all. Both IF and WB were done on RPE1 cells
Date published: 2022-08-27
Rated 5 out of 5 by from great productThis antibody works very well for western blotting for our lab.
Date published: 2022-07-29
Rated 5 out of 5 by from Works greatThis antibody works great in WB in mouse embryonic stem cells.
Date published: 2022-03-17
Rated 4 out of 5 by from good antibodygood antibody clean and strong signal in western blot or IF
Date published: 2021-11-15
Rated 5 out of 5 by from Very good antibodyWe used in WB at 1:200 dilution on K562 whole cell lysates. We obtained a strong and specific signals.
Date published: 2021-07-09
Rated 4 out of 5 by from Good for western blotThe antibody can detect a specific band with 1:250 dilution for western blot. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 5 out of 5 by from anti-cyclin B1 antibody works for western blot.We used the anti-cyclin B1 antibody for western blotting of HeLa whole cell lysate and detected a band of the correct size.
Date published: 2019-07-29
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CCNB1/cyclin B1 抗体 (GNS1) is rated 4.6 out of 5 by 46.
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