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CCNB1/cyclin B1 Antibody (V143.1) - Western Blotting - Image 13874
cyclin B1 抗体 (V143. 1): sc-53235. NIH/3T3 (A), 未处理的 MM-142 (B) 和 PMA 处理的 MM-142 (C) 核提取物中细胞周期蛋白 B1 表达的 Western 印迹分析.
CCNB1/cyclin B1 Antibody (V143.1) - Western Blotting - Image 13874
cyclin B1 抗体 (V143. 1): sc-53235. NIH/3T3 (A), 未处理的 MM-142 (B) 和 PMA 处理的 MM-142 (C) 核提取物中细胞周期蛋白 B1 表达的 Western 印迹分析.
CCNB1/cyclin B1 Antibody (V143.1) - Western Blotting - Image 377721
cyclin B1 抗体 (V143. 1): sc-53235. 对 M1 全细胞裂解液中细胞周期蛋白 B1 表达的 Western 印迹分析.
CCNB1/cyclin B1 Antibody (V143.1) - Western Blotting - Image 41867
cyclin B1 抗体 (V143. 1): sc-53235. 对 K-562 全细胞裂解液中细胞周期蛋白 B1 的表达进行 Western 印迹分析.
cyclin B1 抗体 (V143. 1): sc-53235. NIH/3T3 (A), 未处理的 MM-142 (B) 和 PMA 处理的 MM-142 (C) 核提取物中细胞周期蛋白 B1 表达的 Western 印迹分析.

CCNB1/cyclin B1 抗体 (V143.1): sc-53235

4.0(1)
寫評論提問

说明书
  • CCNB1/cyclin B1 抗体 V143.1 是小鼠单克隆 IgG1，提供 200 µg/ml
  • 针对hamster cyclin B1
  • 推荐用于 mouse, rat 和 human 来源的cyclin B1 WB 和 IP检测
  • 看 CCNB1/cyclin B1 (GNS1): sc-245，可查看有标记的 CCNB1/cyclin B1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP是CCNB1/cyclin B1 Antibody (V143.1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CCNB1/cyclin B1 Antibody (V143.1) 搭配使用（请参阅下面的订购信息）。

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    関連項目

    额外Cyclin抗体，包括cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2, cyclin D3, cyclin E, cyclin E2, cyclin F, cyclin G1, cyclin G2和cyclin H

    在真核细胞中，有丝分裂是由一种被称为促成熟因子、M期特异性组蛋白激酶或M期激酶的蛋白激酶激活后开始的。这种蛋白激酶由催化亚基（Cdc2）、调节亚基（cyclin B）和低分子量亚基（p13-Suc 1）组成。Cdc/cyclin酶受到多种水平的控制，其中由酪氨酸磷酸化调节催化亚基的调控是最好理解的。酪氨酸磷酸化抑制Cdc2/cyclin B酶；在细胞分裂开始时发生的酪氨酸去磷酸化直接激活了预MPF复合物。证据表明，B型cyclin不仅对M期调节亚基的Cdc2蛋白激酶有作用，而且还激活内源性酪氨酸磷酸酶Cdc25A和Cdc25B，其中Cdc2是生理底物。这种效应的特异性表现为cyclin A或cyclin D1无法显示任何对Cdc25A或Cdc25B的刺激。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    CCNB1/cyclin B1 抗体 (V143.1)

    sc-53235
    200 µg/ml
    		RMB2377.00

    CCNB1/cyclin B1 (V143.1): m-IgG Fc BP-HRP 套装

    sc-538981
    200 µg Ab; 10 µg BP
    		RMB2662.00

    why do we have two mirror image of cyclin B1 expression

    Asked by: RACHEAL
    Thank you for your question. Please reach out to our Technical Service Department, we are happy to help with your Cyclin B1 expression inquiry.
    Answered by: Tech Service
    Date published: 2019-04-12

    What is the recommended starting dilution for Western blot for cyclin B1 (V143.1): sc-53235 monoclonal antibody?

    Asked by: jerojero
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-53235.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Worked for WB but bands were faint and blotWorked for WB but bands were faint and blot was messy with NIH/3T3, untreated MM-142 and PMA treated MM-142 nuclear extracts.
    Date published: 2014-09-25
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    CCNB1/cyclin B1 抗体 (V143.1) is rated 4.0 out of 5 by 1.
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