Date published: 2025-9-7
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CCNB1/cyclin B1 Antibody (D-11) - Western Blotting - Image 6547
细胞周期蛋白 B1 抗体 (D-11): sc-7393. 未处理 (A, C) 和依托泊苷处理 (B, D) 的 HeLa (A, B) 和 K-562 (C, D) 全细胞裂解液中细胞周期蛋白 B1 表达的 Western 印迹分析.
细胞周期蛋白 B1 抗体 (D-11): sc-7393. 未处理 (A, C) 和依托泊苷处理 (B, D) 的 HeLa (A, B) 和 K-562 (C, D) 全细胞裂解液中细胞周期蛋白 B1 表达的 Western 印迹分析.

CCNB1/cyclin B1 抗体 (D-11): sc-7393

4.5(11)
寫評論提問

说明书
  • CCNB1/cyclin B1抗体(D-11)是小鼠单克隆IgG1 κ, 在116篇文献中引用，规格为200 µg/ml
  • 免疫1-433氨基酸，对应于human来源cyclin B1全长
  • 抗-CCNB1/cyclin B1 抗体 (D-11) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 cyclin B1
  • 抗CCNB1/cyclin B1抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CCNB1/cyclin B1 (D-11): sc-7393的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CCNB1/cyclin B1 抗体 (D-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CCNB1/cyclin B1 抗体 (D-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Cyclin 抗体 包括 cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2和cyclin D3

cyclin B1抗体（D-11）是一种IgG1 κ小鼠单克隆cyclin B1抗体，可通过WB、IP、IF、IHC(P)和FCM来检测小鼠、大鼠和人源的cyclin B1。cyclin B1抗体（D-11）既有非偶联抗cyclin B1抗体形式，也有多种偶联形式的抗cyclin B1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核细胞中，有丝分裂是从一种被称为促成熟因子、m期特异性组蛋白激酶或m期激酶的蛋白激酶激活后开始的。这种蛋白激酶由催化亚基（Cdc2）、调节亚基（cyclin B）和低分子量亚基（p13-Suc 1）组成。Cdc/细胞周期蛋白酶受到多级控制，其中通过酪氨酸磷酸化对催化亚基的调节是最好理解的。酪氨酸磷酸化抑制Cdc2/细胞周期蛋白B酶；在有丝分裂开始时发生的酪氨酸去磷酸化直接激活pre-MPF复合体。有证据表明，B型细胞周期蛋白不仅作用于Cdc2蛋白激酶的M期调节亚基，而且还激活Cdc25A和Cdc25B内源性酪氨酸磷酸酶，其中Cdc2是生理基质。这种效应的特异性表现为细胞周期蛋白A或细胞周期蛋白D1不能显示Cdc25A或Cdc25B的任何刺激。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CCNB1/cyclin B1 抗体 (D-11)

sc-7393
200 µg/ml
RMB2377.00

CCNB1/cyclin B1 (D-11): m-IgG Fc BP-HRP 套装

sc-526501
200 µg Ab; 10 µg BP
RMB2662.00

CCNB1/cyclin B1 (D-11): m-IgG1 BP-HRP 套装

sc-531874
200 µg Ab; 20 µg BP
RMB2662.00

CCNB1/cyclin B1 抗体 (D-11) AC

sc-7393 AC
500 µg/ml, 25% agarose
RMB3129.00

CCNB1/cyclin B1 抗体 (D-11) HRP

sc-7393 HRP
200 µg/ml
RMB2377.00

CCNB1/cyclin B1 抗体 (D-11) FITC

sc-7393 FITC
200 µg/ml
RMB2482.00

CCNB1/cyclin B1 抗体 (D-11) PE

sc-7393 PE
200 µg/ml
RMB2580.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 488

sc-7393 AF488
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 546

sc-7393 AF546
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 594

sc-7393 AF594
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 647

sc-7393 AF647
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 680

sc-7393 AF680
200 µg/ml
RMB2685.00

CCNB1/cyclin B1 抗体 (D-11) Alexa Fluor® 790

sc-7393 AF790
200 µg/ml
RMB2685.00

请问怎么获得SC-7393的免费小样，我想先试用下，如果质量好，我再购买

Asked by: Dr Wang
感谢您的提问。如何获取免费试用装，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Can this cyclin B1 (D-11): sc-7393 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer this antibody directly conjugated a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 3 out of 5 by from I don't knowUsed for Immunoblotting, probed 1:500 in tbs 0.1% tween overnight, it produces a faint signal slightly higher than the expected molecular weight on WB.
Date published: 2021-10-04
Rated 3 out of 5 by from Faint Western Detection in Rat INS-1 Cell Line60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a faint band of ~55 kD. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 4 out of 5 by from 功能很出色两个月前申请试用的小样，条带还不错，位置很正确，用着很顺利，如果正装有小包装，那就更好了～～～～～～
Date published: 2017-12-19
Rated 5 out of 5 by from Happy with the result!I did WB in patient and control Fibroblats. It worked very well! I'm very happy with the result.
Date published: 2017-09-29
Rated 5 out of 5 by from an excellent antibody for WBThis antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Checking for G2/M phaseI was very pleased with my result after using the cyclin B1 antibody (1:500 dilution). I can see the cell cycle trend in my cells after thymidine release.
Date published: 2017-08-24
Rated 5 out of 5 by from Well characterized cyclin B1 monoclonal abWith more than 50 citations in the primary literature, this Cyclin B1 clone is well established and liked in a wide variety of applications.
Date published: 2016-12-30
Rated 5 out of 5 by from Great WB data obtained using HeLa and KGreat WB data obtained using HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-12
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CCNB1/cyclin B1 抗体 (D-11) is rated 4.5 out of 5 by 11.
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