Date published: 2025-9-8
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CXCR-4 Antibody (12G5) - Flow Cytometry - Image 5990
CXCR-4 (12G5L) FITC: sc-12764 FITC. 人体外周血白细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgG2a: sc-2856 设置.
CXCR-4 Antibody (12G5) - Flow Cytometry - Image 5990
CXCR-4 (12G5L) FITC: sc-12764 FITC. 人体外周血白细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgG2a: sc-2856 设置.
CXCR-4 (12G5L) FITC: sc-12764 FITC. 人体外周血白细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgG2a: sc-2856 设置.

CXCR-4 抗体 (12G5): sc-12764

4.9(9)
寫評論提問

说明书
  • CXCR-4抗体(12G5)是小鼠单克隆IgG2a κ, 在26篇文献中引用，规格为200 µg/ml
  • 针对感染CP-MAC的活SUP-T1细胞制备
  • CXCR-4 抗体 (12G5) 推荐用于IP 和 FCM，检测human 来源的 CXCR-4
  • 抗CXCR-4抗体（12G5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物产品，用于在功能研究中阻断CXCR-4，sc-12764 L，200ug/0.1 mL
  • 关于如何获取CXCR-4 (12G5): sc-12764的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是CXCR-4 Antibody (12G5). 的首选辅助检测试剂。该试剂现与CXCR-4 Antibody (12G5) 打包提供（请参阅下面的订购信息）。

快捷链接

描述
基因信息
说明书与实验方案
研究信息

関連項目

CXCR-4抗体（12G5）是一种小鼠单克隆IgG2a kappa轻链抗体，针对活体CP-MAC感染的SUP-T1细胞产生，专门针对人类来源的CXCR-4蛋白，并经过验证可用于免疫沉淀（IP）和流式细胞术（FCM）等应用。CXCR-4是C-X-C趋化因子受体家族的一员，是一种G蛋白偶联受体，具有七个跨膜结构域，可与基质细胞衍生因子-1（SDF-1）相互作用。该受体在细胞信号传导和迁移中起着关键作用，是T细胞和单核细胞的强效趋化因子。CXCR-4/SDF-1轴对许多生理过程至关重要，包括造血、免疫反应和器官发生，以及癌症转移和免疫失调等病理状况。CXCR-4也是T细胞型HIV-1的主要辅助受体，有助于病毒进入目标细胞，而其配体SDF-1已被证明可以抑制HIV-1感染，因此这种受体-配体组合成为抗病毒和免疫学研究的重要焦点。CXCR-4与其他IL-8受体高度同源，在氨基酸水平上具有约37%的序列相似性，但表现出独特的配体特异性和功能作用。CXCR-4 单克隆抗体（12G5）有非结合型和一系列结合型可供选择，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和 Alexa Fluor® 结合物，为不同的研究应用提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CXCR-4 抗体 (12G5)

sc-12764
200 µg/ml
$316.00

CXCR-4 (12G5): m-IgG2a BP-HRP 套装

sc-545912
200 µg Ab; 10 µg BP
$354.00

CXCR-4 抗体 (12G5) L

sc-12764 L
200 µg/0.1 ml
$316.00

CXCR-4 抗体 (12G5) AC

sc-12764 AC
500 µg/ml, 25% agarose
$416.00

CXCR-4 抗体 (12G5) HRP

sc-12764 HRP
200 µg/ml
$316.00

CXCR-4 抗体 (12G5) FITC

sc-12764 FITC
200 µg/ml
$330.00

CXCR-4 抗体 (12G5) PE

sc-12764 PE
200 µg/ml
$343.00

CXCR-4 抗体 (12G5) Alexa Fluor® 488

sc-12764 AF488
200 µg/ml
$357.00

CXCR-4 抗体 (12G5) Alexa Fluor® 546

sc-12764 AF546
200 µg/ml
$357.00

CXCR-4 抗体 (12G5) Alexa Fluor® 594

sc-12764 AF594
200 µg/ml
$357.00

CXCR-4 抗体 (12G5) Alexa Fluor® 647

sc-12764 AF647
200 µg/ml
$357.00

CXCR-4 抗体 (12G5) Alexa Fluor® 680

sc-12764 AF680
200 µg/ml
$357.00

CXCR-4 抗体 (12G5) Alexa Fluor® 790

sc-12764 AF790
200 µg/ml
$357.00

what is the difference between "sc-12764" L and "sc-12764"? is only the concentration? 

Asked by: Jorge37
Thank you for your question. The regular version of CXCR-4 Antibody (12G5): sc-12764 is supplied as 200 µg in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide. The L version is supplied in concentrated formulation at 200 µg antibody in 0.1 ml PBS with no gelatin or sodium azide.
Answered by: Tech Support Europe
Date published: 2020-07-11

I am looking for blocking peptide for this antibody CXCR-4 (12G5). Please suggest to inhibit antibody binding with target.

Asked by: Rick K
Thank you for your question. CXCR-4 Antibody (12G5) is a mouse monoclonal IgG2a raised against living CP-MAC-infected SUP-T1 cells of CXCR-4 of human origin. The antibody is not raised against a synthetic peptide, and therefore no blocking peptide is available. An alternative negative control would be to use an isotype control in lieu of the CSCR-4 specific antibody. We would recommend our normal mouse IgG 2a: sc-3878.
Answered by: Technical Support
Date published: 2017-07-10

Can CXCR-4 (12G5): sc-12764 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CXCR-4 (12G5): sc-12764 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from antibody helps us to studySee a good expression in endosomes, the antibody has high sensitivity and specificity
Date published: 2017-08-16
Rated 5 out of 5 by from StrongBeautiful IP cytoplasmic staining in human tissue, we are glad to use this product.
Date published: 2017-04-07
Rated 5 out of 5 by from EDTA gave best signal to backgroundvarious antigen regimens tested, EDTA gave best signal to background
Date published: 2017-02-12
Rated 5 out of 5 by from Great choice for FCMAwesome results with CXCR-4 Antibody (12G5). Great signal observed by Flow Cytometry.
Date published: 2017-01-21
Rated 4 out of 5 by from We used it in our WB and it worked wellWe used it in our WB and it worked well.
Date published: 2015-07-28
Rated 5 out of 5 by from Published for WB in HeLaPublished for WB in HeLa - SCBT Publication Review
Date published: 2015-06-01
Rated 5 out of 5 by from Flow cytometry published data with mast cellsFlow cytometry published data with mast cells - SCBT Publication Review
Date published: 2015-02-27
Rated 5 out of 5 by from Used in a publication for IHC with humanUsed in a publication for IHC with human and rat testis - SCBT Publication Review
Date published: 2015-02-16
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CXCR-4 抗体 (12G5) is rated 4.9 out of 5 by 9.
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