Date published: 2025-9-8
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CXCR-4 Antibody (4G10) - Western Blotting - Image 395853
CXCR-4 抗体 (4G10) Alexa Fluor® 790: sc-53534 AF790. 直接近红外 Western 印迹分析 C2C12 全细胞裂解液中 CXCR-4 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
CXCR-4 抗体 (4G10) Alexa Fluor® 790: sc-53534 AF790. 直接近红外 Western 印迹分析 C2C12 全细胞裂解液中 CXCR-4 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

CXCR-4 抗体 (4G10): sc-53534

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寫評論提問

说明书
  • CXCR-4 抗体 4G10 是小鼠单克隆 IgG1 κ，CXCR-4抗体, 在90篇文献中引用，规格为200 µg/ml
  • 与N-terminus氨基酸的CXCR-4的human起源
  • CXCR-4 抗体 (4G10) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 CXCR-4
  • 抗CXCR-4抗体（4G10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CXCR-4 (4G10): sc-53534的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 CXCR-4 抗体 (4G10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CXCR-4 抗体 (4G10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CXCR-4 抗体（4G10）是一种小鼠单克隆 IgG1 kappa 轻链抗体，针对人源 CXCR-4 的 N 端氨基酸而制备，对小鼠、大鼠和人源物种均有反应活性，可用于免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、流式细胞仪 (FCM) 和免疫组织化学等应用。CXCR-4又称CD184或Fusin，是一种七跨膜G蛋白偶联受体，主要存在于细胞表面。CXCR-4 在各种组织中高度表达，在细胞信号传导，尤其是免疫和发育过程中起着关键作用。CXCR-4 与基质细胞衍生因子-1（SDF-1）相互作用，介导 T 细胞和单核细胞等免疫细胞的趋化迁移。CXCR-4 位于细胞表面，能有效检测和响应细胞外信号，在免疫反应、组织修复和发育过程中引导细胞移动。CXCR-4 在质膜上的定位使其成为 T 型 HIV-1 的主要共受体，帮助病毒进入宿主细胞，而 SDF-1 则是 HIV-1 感染的抑制剂，在抗病毒研究中具有重要意义。CXCR-4 与癌症进展有关，因为表达和信号传导失调与转移和肿瘤细胞存活有关。抗 CXCR-4 抗体 (4G10) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和 Alexa Fluor® 等，为研究人员探索 CXCR-4 在健康和疾病中的关键作用和细胞动态提供了多功能工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CXCR-4 抗体 (4G10) 参考文献:

  1. 支柱文章：HIV-1 进入辅助因子：七跨膜 G 蛋白偶联受体的功能性 cDNA 克隆。Science.1996.272: 872-877.  |  Feng, Y., et al. 2011. J Immunol. 186: 6076-81. PMID: 21597040
  2. CXC 趋化因子与一组独特的选择性决定因子结合，这些决定因子可以独立发挥作用，并广泛分布在人类白细胞介素-8 受体 B 的多个结构域上。  |  Ahuja, SK., et al. 1996. J Biol Chem. 271: 225-32. PMID: 8550564
  3. 通过一次静脉注射白细胞介素-8，快速调动恒河猴的造血祖细胞。  |  Laterveer, L., et al. 1996. Blood. 87: 781-8. PMID: 8555503
  4. 鉴定 HIV-1 原始分离株的主要共受体。  |  Deng, H., et al. 1996. Nature. 381: 661-6. PMID: 8649511
  5. 淋巴细胞趋化吸引剂 SDF-1 是 LESTR/fusin 的配体，可阻止 HIV-1 进入。  |  Bleul, CC., et al. 1996. Nature. 382: 829-33. PMID: 8752280
  6. HIV-1核心受体的同基因缺陷是一些多重暴露个体抵抗HIV-1感染的原因。  |  Liu, R., et al. 1996. Cell. 86: 367-77. PMID: 8756719
  7. 缺乏 CXC 趋化因子 PBSF/SDF-1 的小鼠 B 细胞淋巴生成和骨髓生成缺陷  |  Nagasawa, T., et al. 1996. Nature. 382: 635-8. PMID: 8757135

订购信息

产品名称产品编号规格价格数量收藏夹

CXCR-4 抗体 (4G10)

sc-53534
200 µg/ml
RMB2377.00

CXCR-4 (4G10): m-IgG Fc BP-HRP 套装

sc-528571
200 µg Ab; 10 µg BP
RMB2662.00

CXCR-4 (4G10): m-IgGκ BP-HRP 套装

sc-520982
200 µg Ab, 40 µg BP
RMB2662.00

CXCR-4 抗体 (4G10) AC

sc-53534 AC
500 µg/ml, 25% agarose
RMB3129.00

CXCR-4 抗体 (4G10) HRP

sc-53534 HRP
200 µg/ml
RMB2377.00

CXCR-4 抗体 (4G10) FITC

sc-53534 FITC
200 µg/ml
RMB2482.00

CXCR-4 抗体 (4G10) PE

sc-53534 PE
200 µg/ml
RMB2580.00

CXCR-4 抗体 (4G10) Alexa Fluor® 488

sc-53534 AF488
200 µg/ml
RMB2685.00

CXCR-4 抗体 (4G10) Alexa Fluor® 546

sc-53534 AF546
200 µg/ml
RMB2685.00

CXCR-4 抗体 (4G10) Alexa Fluor® 594

sc-53534 AF594
200 µg/ml
RMB2685.00

CXCR-4 抗体 (4G10) Alexa Fluor® 647

sc-53534 AF647
200 µg/ml
RMB2685.00

CXCR-4 抗体 (4G10) Alexa Fluor® 680

sc-53534 AF680
200 µg/ml
RMB2685.00

CXCR-4 抗体 (4G10) Alexa Fluor® 790

sc-53534 AF790
200 µg/ml
RMB2685.00

SOURCE是什么？human？

Asked by: Andy
Thanks for your question. CXCR-4 Antibody (4G10):sc-53534 is a mouse monoclonal antibody. 谢谢您的问题。CXCR-4 Antibody (4G10):sc-53534是一种小鼠单克隆抗体。
Answered by: Yanru Tao
Date published: 2025-08-08

Do I need permeabilization for FCM?

Asked by: SAITO
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I work with mouse samples. How can I stain the tissue with a mouse antibody and avoid non-specific staining?

Asked by: Cweed
We recommend staining with directly conjugated primary antibodies. SC-53534 is available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-21

What secondary detection reagent do you recommend for this antibody?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-21
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Rated 5 out of 5 by from good for IPA nice band when applied in IP sample of cells O.E. CXCR4 and human platelets
Date published: 2020-10-07
Rated 5 out of 5 by from Great compactibilityAs per the review, it sounds Great. The Staining is also really clear
Date published: 2019-03-01
Rated 5 out of 5 by from It's easy to dyeI applied this antibody to IHC (1:300) mice and we found that the CXCR-4 antibodies had a clear staining. It's very nice.
Date published: 2017-10-31
Rated 5 out of 5 by from Works great.Anti-CXCR4 staining in paraffin embeded sections worked very well. Good staining result with low background.
Date published: 2017-10-30
Rated 5 out of 5 by from works very wellI have used this antibody on 20 um spinal cord cryo-sections at a dilution of 1:500. It works very well and shows low background staining along with intense cytoplasm and nuclear staining of cells.
Date published: 2017-08-19
Rated 3 out of 5 by from goodAntibody sample, 1:200 dilution for immunostaining of paraffin-embedded sections from a xenograft model of oral cancer, which showing cytoplasmic staining of cells.
Date published: 2017-08-18
Rated 5 out of 5 by from western blot got strong bandUsed in western blot for CXCR-4 protein analysis,storng band and Low background was observed.Used with a dilution of 1:4000.
Date published: 2017-08-12
Rated 5 out of 5 by from Good control.The antibody works well in WB but over time with more use, I found a strong signal，Good control
Date published: 2017-06-06
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CXCR-4 抗体 (4G10) is rated 4.9 out of 5 by 21.
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