Date published: 2025-9-8
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CX3CR1 Antibody (B-7) - Western Blotting - Image 402133
CX3CR1 抗体 (B-7): sc-377227. THP-1 全细胞裂解液中 CX3CR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG2a BP-CFL 680: sc-542739.
CX3CR1 Antibody (B-7) - Western Blotting - Image 402133
CX3CR1 抗体 (B-7): sc-377227. THP-1 全细胞裂解液中 CX3CR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG2a BP-CFL 680: sc-542739.
CX3CR1 Antibody (B-7) - Western Blotting - Image 282553
CX3CR1 抗体 (B-7): sc-377227. THP-1 全细胞裂解液中 CX3CR1 表达的 Western 印迹分析.
CX3CR1 Antibody (B-7) - Western Blotting - Image 402134
CX3CR1 抗体 (B-7): sc-377227. THP-1 全细胞裂解液中 CX3CR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG2a BP-CFL 790: sc-542740.
CX3CR1 抗体 (B-7): sc-377227. THP-1 全细胞裂解液中 CX3CR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG2a BP-CFL 680: sc-542739.

CX3CR1 抗体 (B-7): sc-377227

4.3(7)
寫評論提問

说明书
  • CX3CR1 抗体 B-7 是小鼠单克隆 IgG2a κ，CX3CR1抗体, 在11篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列131-200位于human物种的CX3CR1的内部区域内
  • CX3CR1 抗体 (B-7) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 CX3CR1
  • 抗CX3CR1抗体（B-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CX3CR1 (B-7): sc-377227的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是CX3CR1 Antibody (B-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CX3CR1 Antibody (B-7) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

CX3CR1抗体（B-7）是一种IgG2a κ小鼠单克隆CX3CR1抗体（也被称作C-X3-C趋化因子受体1抗体、CCRL1抗体、趋化因子（C-X3-C模体）受体1抗体、孤儿G蛋白偶联受体编码基因V28抗体、GPRV28抗体、G蛋白偶联受体13抗体、GPR13抗体、β趋化因子受体样1抗体、CCRL1抗体或CMKBRL1抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测到人源的CX3CR1蛋白。非偶联形式的抗CX3CR1抗体以及多种偶联形式的抗CX3CR1抗体（包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物）均可作为CX3CR1抗体（B-7）使用。趋化因子是具有化学吸引作用的蛋白质，根据半胱氨酸特征模体（称为C、CC、CXC和CX3C）可分为不同的亚家族。Fractalkine（也被称作CX3CL1）含有CX3C模体，在大脑中广泛表达，并在内皮细胞中响应炎症信号（如LPS、IL-1、TNF和CD40L）而上调。Fractalkine既以膜结合形式存在，也以趋化可溶性形式存在，并能以高亲和力与其同源受体CX3CR1结合，诱导白细胞粘附和迁移或趋化功能。CX3CR1以前被命名为V28和趋化因子β受体样1（CMKBRL1），在中性粒细胞、单核细胞、T淋巴细胞和包括大脑在内的多个器官中表达。CX3CR1还与CD4一起作为HIV-1病毒包膜蛋白的共受体，携带CX3CR1变体单倍型纯合子的患者比其他单倍型的患者更快地发展为艾滋病。CX3CR1也可能参与动脉粥样硬化性冠状动脉疾病（CAD）的发病机理，并被认为是内皮相关炎症性疾病治疗干预的潜在药物靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CX3CR1 抗体 (B-7) 参考文献:

  1. 通过免疫中和分叉碱受体 CX3CR1 快速通讯预防新月体性肾小球肾炎。  |  Feng, L., et al. 1999. Kidney Int. 56: 612-20. PMID: 10432400
  2. 患有趋化因子受体 CX3CR1 结构变异的 HIV 感染者迅速发展为艾滋病。  |  Faure, S., et al. 2000. Science. 287: 2274-7. PMID: 10731151
  3. 神经元上 CX3CR1 趋化因子受体的表达及其在神经元存活中的作用  |  Meucci, O., et al. 2000. Proc Natl Acad Sci U S A. 97: 8075-80. PMID: 10869418
  4. 作为冠状动脉疾病遗传风险因素的断裂碱受体 CX3CR1 的多态性。  |  Moatti, D., et al. 2001. Blood. 97: 1925-8. PMID: 11264153
  5. Fractalkine (CX3CL1)是极化 Th1 反应的放大电路。  |  Fraticelli, P., et al. 2001. J Clin Invest. 107: 1173-81. PMID: 11342581
  6. 呼吸道合胞病毒 G 糖蛋白对 CX3C 趋化因子的模拟作用  |  Tripp, RA., et al. 2001. Nat Immunol. 2: 732-8. PMID: 11477410
  7. 肿瘤坏死因子-α-转化酶介导了分叉蛋白的诱导性裂解。  |  Tsou, CL., et al. 2001. J Biol Chem. 276: 44622-6. PMID: 11571300
  8. 神经系统疾病中 CX3CL1/CX3CR1 轴的影响。  |  Pawelec, P., et al. 2020. Cells. 9: PMID: 33065974
  9. 治疗神经退行性疾病的 CX3CL1/CX3CR1 信号靶点。  |  Subbarayan, MS., et al. 2022. Pharmacol Ther. 231: 107989. PMID: 34492237
  10. Fractalkine 可通过 CX3CR1/AMPK/PPARγ 通路促进 GMH 后血肿消退并改善神经功能  |  Chen, X., et al. 2023. Stroke. 54: 2420-2433. PMID: 37465997
  11. 人 beta 趋化因子受体样基因的克隆, 染色体定位和 RNA 表达。  |  Combadiere, C., et al. 1995. DNA Cell Biol. 14: 673-80. PMID: 7646814
  12. 鉴定 CX3CR1。人类 CX3C 趋化因子分叉碱的趋化受体和 HIV-1 的融合核心受体。  |  Combadiere, C., et al. 1998. J Biol Chem. 273: 23799-804. PMID: 9726990

订购信息

产品名称产品编号规格价格数量收藏夹

CX3CR1 抗体 (B-7)

sc-377227
200 µg/ml
RMB2377.00

CX3CR1 (B-7): m-IgG2a BP-HRP 套装

sc-545953
200 µg Ab; 10 µg BP
RMB2662.00

CX3CR1 抗体 (B-7) AC

sc-377227 AC
500 µg/ml, 25% agarose
RMB3129.00

CX3CR1 抗体 (B-7) HRP

sc-377227 HRP
200 µg/ml
RMB2377.00

CX3CR1 抗体 (B-7) FITC

sc-377227 FITC
200 µg/ml
RMB2482.00

CX3CR1 抗体 (B-7) PE

sc-377227 PE
200 µg/ml
RMB2580.00

CX3CR1 抗体 (B-7) Alexa Fluor® 488

sc-377227 AF488
200 µg/ml
RMB2685.00

CX3CR1 抗体 (B-7) Alexa Fluor® 546

sc-377227 AF546
200 µg/ml
RMB2685.00

CX3CR1 抗体 (B-7) Alexa Fluor® 594

sc-377227 AF594
200 µg/ml
RMB2685.00

CX3CR1 抗体 (B-7) Alexa Fluor® 647

sc-377227 AF647
200 µg/ml
RMB2685.00

CX3CR1 抗体 (B-7) Alexa Fluor® 680

sc-377227 AF680
200 µg/ml
RMB2685.00

CX3CR1 抗体 (B-7) Alexa Fluor® 790

sc-377227 AF790
200 µg/ml
RMB2685.00

This antibody is recommended for detection of CX3CR1 of human origin,but the references used it to detect CX3CR1 in rat or murine sample.I want to know if it is suitable for rat or murine.

Asked by: xiaoting
感谢您的提问。sc-377227推荐检测human 来源的 CX3CR1。如果您的样本与文献中的相同，并且文献用于大鼠与小鼠，则也可以提供质保。谢谢。 Thank you for your question. sc-377227 recommends testing CX3CR1 of human origin. If your sample is the same as in the references and references were also used in rat and mouse,can also provide a warranty. Thank you.
Answered by: Jiajun Z
Date published: 2023-07-27

“Good antibody for Microglial cells staining We used the antibody to stain microglial cells in frozen slice of mouse cortex tissue” Can I get email or phone about this user? I cannot have the positive signals as this description, thank you !

Asked by: satima
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I am not sure which of your isotype controls I should use for this antibody. Could you help me?

Asked by: s2madrob
Thank you for your question. Anti-CX3CR1 Antibody (B-7) is a mouse monoclonal IgG2a, so the appropriate isotype control would be normal mouse IgG2a: sc-3878. Other isotype controls and their conjugates can be found under this link: https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah
Answered by: Tech Support Europe
Date published: 2020-07-09

Is this primary suitable for IHC AP-secondary antibody？

Asked by: Allen95
Thank you for your question. This CX3CR1 antibody is recommended for Immunofluorescence on cells or on fresh frozen tissues, but has not been tested for IHC on paraffin tissue sections. Any anti-mouse secondary antibody should be fine.
Answered by: Tech Service
Date published: 2019-09-13

Hi, i can use CX3CR1 B-7 for detection of CX3CR1 of rat by Western Blotting? I used CX3CR1 H -70 for detection of CX3Cr1 of rat by WB before and i get excellents resulta before!!! If i can't use this antibody, what You recommend for detection in rat?

Asked by: Ferrara
Thank you for your question. This antibody has not been tested for use on rat samples so it is not warrantied for that use. It may work, but we have not tested it in house yet so we can not warranty it for use on rat samples. If you are interested in a sample of the antibody to test in on your rat cells, please contact your local distributor and they will be happy to help you. You can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-10-18

Hi , Is this antibody suitable for Flow cytometery? If yes, I did not see any histogram/dotblot pictures!

Asked by: Fari
Thank you for your question. We have not yet tested this mouse monoclonal, CX3CR1 Antibody (B-7): sc-377227, for flow cytometry, so this application (FCM) is not recommended at this time. This antibody is currently recommended for detection of CX3CR1 of human origin by WB, IP, IF and ELISA.
Answered by: Technical Support
Date published: 2017-04-18

What is the recommended starting dilution for Western blot for sc-377227: CX3CR1 (B-7) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

Can this antibody be used for IFto tissue of rat?

Asked by: Longyu
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Rated 3 out of 5 by from Decent signal in iPSC-derived microgliaThis antibody recognizing CX3CR1 was tested in human pluripotent stem cells-derived microglia-like cells by immunofluorescence at 1:100 dilution. Staining resulted in a faint signal with a good signal to noise ratio.
Date published: 2023-12-21
Rated 4 out of 5 by from good antibody for IFI used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by from 抗体是好用的抗体是好用的，用于人PMA-primed THP-1细胞，稀释比例为1:100，上样量为40ug 一次就跑出来了
Date published: 2017-12-26
Rated 4 out of 5 by from Good antibody for Microglial cells stainingWe used the antibody to stain microglial cells in frozen slice of mouse cortex tissue.
Date published: 2017-04-11
Rated 5 out of 5 by from successflully labeled microgliaRat L5 spinal cord was lesioned on one side. The CX3CR1-labeled microglia was significantly induced compared to the un-lesion control side.
Date published: 2017-02-15
Rated 4 out of 5 by from not suit to IHC-PWe didnot see expected staining pattern in human decidua by IHC-P with high backgroud
Date published: 2016-12-12
Rated 5 out of 5 by from Produced positive Western blot data of CX3CR1Produced positive Western blot data of CX3CR1 expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2013-10-24
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CX3CR1 抗体 (B-7) is rated 4.3 out of 5 by 7.
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