Date published: 2025-9-8
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CRY1 Antibody (W-L5) - Western Blotting - Image 34369
CRY1 抗体 (W-L5): sc-101006. 对 HeLa 核提取物中 CRY1 的表达进行 Western 印迹分析.
CRY1 抗体 (W-L5): sc-101006. 对 HeLa 核提取物中 CRY1 的表达进行 Western 印迹分析.

CRY1 抗体 (W-L5): sc-101006

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说明书
  • CRY1抗体(W-L5)是小鼠单克隆IgG1 κ, 在5篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组CRY1
  • 推荐用于 mouse, rat 和 human 来源的CRY1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取CRY1 (W-L5): sc-101006的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CRY1 抗体 (W-L5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CRY1 抗体 (W-L5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CRY1抗体（W-L5）是一种IgG1 κ小鼠单克隆CRY1抗体（也称为CRY1抗体或隐花色素-1抗体），它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的CRY1。CRY1抗体（W-L5）可作为非共轭抗CRY1抗体形式提供。昼夜节律钟是调节激素节律、睡眠周期和进食行为的生物计时器。这些节律是在视交叉上核（SCN）中产生的，SCN是一个位于大脑内的细胞自主昼夜节律振荡器，它通过光线与环境同步。包括Clock和BMAL1在内的许多转录因子是SCN的分子组成部分，它们诱导参与光/暗周期夹带的蛋白质的表达，其中包括Per1和Per2。Tim（代表timeless）产生一个负反馈回路，通过抑制Clock目标基因的表达来调节Clock的活性。Tim与Per1和Per2形成异二聚体，这些异二聚体与Clock结合并阻止Clock-BMAL1二聚体的激活，以抑制Per基因的表达。此外，作为昼夜节律钟的隐花色素光感受器的CRY蛋白，作为光独立抑制剂发挥抑制昼夜节律钟的作用。CRY1和CRY2通过与激活剂Clock-BMAL1以及各种反馈抑制剂Per1、Per2和Tim结合，负调控SCN的组成部分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CRY1 抗体 (W-L5) 参考文献:

  1. 隐色体 1 和 2 对哺乳动物周期基因和昼夜节律的不同调控。  |  Vitaterna, MH., et al. 1999. Proc Natl Acad Sci U S A. 96: 12114-9. PMID: 10518585
  2. CRY1和CRY2在哺乳动物昼夜节律钟中的作用与光无关。  |  Griffin, EA., et al. 1999. Science. 286: 768-71. PMID: 10531061
  3. 家族性睡眠时相延迟症中的人类昼夜节律钟基因 CRY1 变异  |  Patke, A., et al. 2017. Cell. 169: 203-215.e13. PMID: 28388406
  4. CRY1-CBS 结合调节昼夜节律钟功能和新陈代谢。  |  Cal-Kayitmazbatir, S., et al. 2021. FEBS J. 288: 614-639. PMID: 32383312
  5. 肝GSK3β依赖性CRY1降解导致糖尿病高血糖。  |  Kim, YY., et al. 2022. Diabetes. 71: 1373-1387. PMID: 35476750
  6. 昼夜节律CRY1调节多能干细胞特性和体细胞重编程。  |  Sato, S., et al. 2023. Cell Rep. 42: 112590. PMID: 37261952
  7. 哺乳动物视网膜中存在 24 小时昼夜节律时钟。  |  Morell, V. 1996. Science. 272: 349. PMID: 8602520
  8. 哺乳动物的两种假定昼夜节律调节器 mper1 和 mper2 对光的不同反应。  |  Albrecht, U., et al. 1997. Cell. 91: 1055-64. PMID: 9428527
  9. 哺乳动物昼夜节律自律环：永恒同源物和 mPer1 相互作用并负向调节 CLOCK-BMAL1 诱导的转录。  |  Sangoram, AM., et al. 1998. Neuron. 21: 1101-13. PMID: 9856465
  10. 哺乳动物永恒的分子分析  |  Zylka, MJ., et al. 1998. Neuron. 21: 1115-22. PMID: 9856466
  11. 昼夜节律钟的分子基础  |  Dunlap, JC. 1999. Cell. 96: 271-90. PMID: 9988221
  12. 调节昼夜节律上钟节律输出的分子机制  |  Jin, X., et al. 1999. Cell. 96: 57-68. PMID: 9989497

订购信息

产品名称产品编号规格价格数量收藏夹

CRY1 抗体 (W-L5)

sc-101006
100 µg/ml
RMB2504.00

CRY1 (W-L5): m-IgG Fc BP-HRP 套装

sc-539728
100 µg Ab; 10 µg BP
RMB2662.00

CRY1 (W-L5): m-IgG1 BP-HRP 套装

sc-541674
100 µg Ab; 20 µg BP
RMB2662.00

Can sc-101006: CRY1 (W-L5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-101006: CRY1 (W-L5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24

I have been having problems producing clean blots with specific bands with this antibody. Is anyone here willing to share their blotting procedure? Thanks in advance!

Asked by: Tyrsett
Thank you for your question. We recommend blocking with Blotto (5% nonfat milk in TBST) for one hour at room temperature, but you can try overnight at 4C if you are experiencing high background. We then recommend diluting the primary antibody in fresh Blotto at a dilution of 1:200 and incubating for one hour at room temperature. We load 50 µg sample protein. And to help reduce non-specific banding, we recommend using our specific mouse IgG kappa binding protein (sc-516102) in place of a conventional anti-mouse IgG secondary antibody. A link to our complete recommended protocol is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting If you continue to have trouble, please contact our Technical Service Department and we can assist you with troubleshooting the antibody.
Answered by: Technical Support
Date published: 2021-01-05
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Rated 1 out of 5 by from barely detectable by WBI have tested this antibody WB of mouse hippocampus and could only observe an extremely faint band of the expected size, after long exposure and high antibody concentration
Date published: 2018-10-24
Rated 5 out of 5 by from perfect resultCry1 is a circadian clock gene,we use this antibody got the Conclusion :Cry1 is increased in poorly differentiated adenocarcinoma,It's very statisfied result use 1:800.
Date published: 2017-07-15
Rated 5 out of 5 by from Good productThe CRY1 Antibody (W-L5): sc-101006 works well for IHC applications with formalin fixed, paraffin-embedded tissue sections showing staining pattern localized to the nucleus and cytoplasm.
Date published: 2017-02-03
Rated 4 out of 5 by from Solid WB signal with a minimum of nonSolid WB signal with a minimum of non-specific bands!
Date published: 2015-11-16
Rated 4 out of 5 by from Band detected at expected mw in HeLa nuclearBand detected at expected mw in HeLa nuclear extract. -SCBT QC
Date published: 2015-05-04
Rated 4 out of 5 by from Produced great immunoperoxidase nuclear andProduced great immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. -SCBT QC
Date published: 2015-04-29
Rated 4 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using human CRY1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from ClearClear, specific band observed in WB in mouse CRY1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-25
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CRY1抗体 (W-L5) is rated 3.9 out of 5 by 8.
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