Date published: 2025-9-7

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CREST 抗体 (D-7): sc-515827

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说明书
  • CREST 抗体 D-7 是小鼠单克隆 IgG2b κ,CREST抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的的内部区域内的氨基酸287-312之间
  • CREST 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CREST
  • 抗CREST抗体(D-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CREST (D-7): sc-515827免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 CREST 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CREST 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CREST抗体(D-7)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的CREST蛋白。CREST (D-7) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。钙反应转激活蛋白(CREST,SS18L1)对于神经元树突的正常发育至关重要,因为CREST定位于核体,并在神经元形态发生中发挥重要作用。CREST包含一个多功能域,对于转录激活、核体定位和二聚化至关重要,而这些对于调节功能至关重要。值得注意的是,CREST与cAMP反应元件结合蛋白(CREB)结合蛋白(CBP)相互作用,这凸显了CREST在调节神经元生长和发育中的重要性。CREST的表达无处不在,在脾脏中的水平最低,研究表明,靶向破坏Ss18l1(CREST)基因的小鼠虽然表现出皮质和海马树突发育缺陷,但仍然存活。这一发现强调了钙激活CREST介导的转录在调节神经元形态发生中的关键作用,因为CREST突变小鼠的皮质神经元表现出钙依赖性树突生长受损。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CREST 参考文献:

  1. 小鼠和人类 SS18 基因, 两个类似于人类 SS18 的基因以及小鼠 Ss18 伪基因的图谱绘制和特征描述。  |  de Bruijn, DR., et al. 2001. Cytogenet Cell Genet. 92: 310-9. PMID: 11435705
  2. 滑膜肉瘤中的新型融合基因 SS18L1/SSX1  |  Storlazzi, CT., et al. 2003. Genes Chromosomes Cancer. 37: 195-200. PMID: 12696068
  3. 由钙调控转录激活因子 CREST 调控的树突发育。  |  Aizawa, H., et al. 2004. Science. 303: 197-202. PMID: 14716005
  4. 钙响应式反式激活因子将 CREB 结合蛋白招募到核体上。  |  Pradhan, A. and Liu, Y. 2004. Neurosci Lett. 370: 191-5. PMID: 15488321
  5. 抑制培养神经元树突生长的钙响应式转录因子(CREST)的多功能结构域。  |  Pradhan, A. and Liu, Y. 2005. J Biol Chem. 280: 24738-43. PMID: 15866867
  6. 人类滑膜肉瘤相关 SS18 和 SS18L1 基因位点的共同起源。  |  de Bruijn, DR. and Geurts van Kessel, A. 2006. Cytogenet Genome Res. 112: 222-6. PMID: 16484776
  7. 系统性硬化症相关钙化症的治疗:利妥昔单抗诱导 CREST 相关钙化症消退的病例报告和文献综述。  |  Daoussis, D., et al. 2012. Semin Arthritis Rheum. 41: 822-9. PMID: 22221908
  8. 针对鹌鹑酪氨酸羟化酶的单克隆抗体:描述并用于神经嵴分化细胞的免疫细胞化学研究。  |  Fauquet, M. and Ziller, C. 1989. J Histochem Cytochem. 37: 1197-205. PMID: 2569003
  9. 药物诱导的抗中心粒抗体?  |  Haberhauer, G. and Bröll, H. 1989. Z Rheumatol. 48: 99-100. PMID: 2786663
  10. 将新技术应用于神经嵴迁移和分化研究。  |  Bronner-Fraser, M. and Fraser, SE. 1988. Am J Med Genet Suppl. 4: 23-39. PMID: 3144984
  11. 神经源前体在神经嵴细胞系内的分化。  |  Marusich, MF. 1993. Brain Res Bull. 30: 257-63. PMID: 8457874
  12. 预测人类不明基因的编码序列。X. 100 个新的大脑 cDNA 克隆的完整序列,这些基因可在体外编码大型蛋白质。  |  Ishikawa, K., et al. 1998. DNA Res. 5: 169-76. PMID: 9734811

订购信息

产品名称产品编号规格价格数量收藏夹

CREST 抗体 (D-7)

sc-515827
200 µg/ml
RMB2377.00

CREST (D-7): m-IgG Fc BP-HRP 套装

sc-531530
200 µg Ab; 10 µg BP
RMB2662.00

CREST (D-7): m-IgGκ BP-HRP 套装

sc-525241
200 µg Ab, 40 µg BP
RMB2662.00

CREST (D-7): m-IgG2b BP-HRP 套装

sc-550294
200 µg Ab; 10 µg BP
RMB2662.00

CREST 抗体 (D-7) AC

sc-515827 AC
500 µg/ml, 25% agarose
RMB3129.00

CREST 抗体 (D-7) HRP

sc-515827 HRP
200 µg/ml
RMB2377.00

CREST 抗体 (D-7) FITC

sc-515827 FITC
200 µg/ml
RMB2482.00

CREST 抗体 (D-7) PE

sc-515827 PE
200 µg/ml
RMB2580.00

CREST 抗体 (D-7) Alexa Fluor® 488

sc-515827 AF488
200 µg/ml
RMB2685.00

CREST 抗体 (D-7) Alexa Fluor® 546

sc-515827 AF546
200 µg/ml
RMB2685.00

CREST 抗体 (D-7) Alexa Fluor® 594

sc-515827 AF594
200 µg/ml
RMB2685.00

CREST 抗体 (D-7) Alexa Fluor® 647

sc-515827 AF647
200 µg/ml
RMB2685.00

CREST 抗体 (D-7) Alexa Fluor® 680

sc-515827 AF680
200 µg/ml
RMB2685.00

CREST 抗体 (D-7) Alexa Fluor® 790

sc-515827 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if CREST (D-7): sc-515827 mouse monoclonal antibody will work?

Asked by: TinTin
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Good for Western BlotAntibody detects CREST in SK-N-MC, K-562, and NIH/3T3 nuclear extracts and SK-N-SH, Neuro-2A, and C6 cell lysates by Western blot. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Great for WB and can IP but be careful...The old Crest M15 goat (C-terminal) antibody from Santa Cruz was excellent for co-immunoprecipitating the full BAF complex. This new antibody co-immunoprecipitates Baf53b and Baf47 well but seems to interfere with binding to Baf170 and Brg1/Brm.
Date published: 2020-02-07
Rated 5 out of 5 by from Great antibody for western blotOne single clear band. I used this antibody at 1:500 dilution with 5% carnation milk in TBST with a goat anti mouse light chain specific HRP.
Date published: 2018-05-25
Rated 1 out of 5 by from This is not a good antibody for IF in mouse oocyteI tried this antibody using mouse oocyte for two times, but there was no signal. The picture was added in another antibody's review.
Date published: 2017-07-24
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CREST抗体 (D-7) is rated 3.8 out of 5 by 4.
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