Date published: 2025-9-7

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CREM 抗体 (C-2): sc-390426

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说明书
  • CREM抗体(C-2)是小鼠单克隆IgG2a κ, 在7篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-153位于mouse物种的CREM的N-terminus
  • 抗-CREM 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CREM
  • 抗CREM抗体(C-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CREM (C-2): sc-390426免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 CREM 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CREM 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CREM抗体(C-2)是一种IgG2a κ小鼠单克隆CREM抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的CREM。CREM抗体(C-2)既可以是非偶联的抗CREM抗体形式,也可以是多种偶联形式的抗CREM抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核基因转录受到序列特异性转录因子的调控,这些转录因子与模块化的顺式作用启动子和增强子元件结合。ATF/CREB转录因子家族结合回文cAMP应答元件(CRE)的八核苷酸TGACGTCA。ATF/CREB家族包括CREM、CREB-1、CREB-2(也称为ATF-4)、ATF-1、ATF-2和ATF-3。这类蛋白含有高度分化的N端结构域,但共享一个用于二聚化和DNA结合的C端亮氨酸拉链。已知转录因子cAMP反应元件调节器(CREM)对男性生育能力起着至关重要的作用,因为已经证明缺乏功能性CREM基因的雄性小鼠是不育的。在睾丸中,CREM的转录活性通过与组织特异性伴侣--睾丸中CREM的激活剂(ACT)的相互作用而受到控制,这赋予了它强大且不依赖磷酸化的激活能力。发现ACT的功能受到睾丸特异性驱动蛋白KIF17b的调节。此外,该抗体还与犬和叙利亚仓鼠有反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CREM 抗体 (C-2) 参考文献:

  1. 雄性生殖细胞基因转录的特殊规则:CREM 范式。  |  Monaco, L., et al. 2004. Int J Androl. 27: 322-7. PMID: 15595950
  2. 人类雄性生殖细胞中 CREM 激活剂和抑制剂同工酶的表达。  |  Blöcher, S., et al. 2005. Int J Androl. 28: 215-23. PMID: 16048633
  3. 转录因子 ATF cDNA 克隆:能够选择性地形成 DNA 结合异二聚体的大量亮氨酸拉链蛋白家族。  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
  4. 一种常见的细胞转录因子 ATF 与 E1a 和环 AMP 诱导的启动子中的调控元件相互作用。  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
  5. 鉴定大鼠体生长抑素基因中的环-AMP 反应元件。  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459

订购信息

产品名称产品编号规格价格数量收藏夹

CREM 抗体 (C-2)

sc-390426
200 µg/ml
RMB2377.00

CREM (C-2): m-IgG Fc BP-HRP 套装

sc-526159
200 µg Ab; 10 µg BP
RMB2662.00

CREM (C-2): m-IgG2a BP-HRP 套装

sc-546517
200 µg Ab; 10 µg BP
RMB2662.00

CREM 抗体 (C-2) AC

sc-390426 AC
500 µg/ml, 25% agarose
RMB3129.00

CREM 抗体 (C-2) HRP

sc-390426 HRP
200 µg/ml
RMB2377.00

CREM 抗体 (C-2) FITC

sc-390426 FITC
200 µg/ml
RMB2482.00

CREM 抗体 (C-2) PE

sc-390426 PE
200 µg/ml
RMB2580.00

CREM 抗体 (C-2) Alexa Fluor® 488

sc-390426 AF488
200 µg/ml
RMB2685.00

CREM 抗体 (C-2) Alexa Fluor® 546

sc-390426 AF546
200 µg/ml
RMB2685.00

CREM 抗体 (C-2) Alexa Fluor® 594

sc-390426 AF594
200 µg/ml
RMB2685.00

CREM 抗体 (C-2) Alexa Fluor® 647

sc-390426 AF647
200 µg/ml
RMB2685.00

CREM 抗体 (C-2) Alexa Fluor® 680

sc-390426 AF680
200 µg/ml
RMB2685.00

CREM 抗体 (C-2) Alexa Fluor® 790

sc-390426 AF790
200 µg/ml
RMB2685.00

How can CREM (C-2): sc-390426 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-390426 HRP), phycoerythrin (sc-390426 PE), fluoroscein (sc-390426 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25

Does it also detect ICER (1pprox. 20kDa band) on a western blot?

Asked by: mayu
Thank you for your question. We have not specifically tested this antibody to determine if it can also detect the isoform known as ICER. CREM Antibody (C-2) is a mouse monoclonal raised against amino acids 1-153 mapping at the N-terminus of CREM of mouse origin. The exact epitope recognized by the antibody has not been determined. There are many isoforms described for CREM, and most are modified within the range of the immunogen for this antibody. This would have to be determined experimentally.
Answered by: Technical Support
Date published: 2022-07-12
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Rated 4 out of 5 by from Recognizes all CREB family members in WBI overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. CREM C2 (dilution 1:200) recognized GFP-CREM, but was not specific to CREM in WB, as it recognised all the GFP-tagged CREB family members. Also recognizes endogenous levels of CREB in rat primary cortical neuron lysates.
Date published: 2018-08-23
Rated 5 out of 5 by from Excellent immunofluorescence nuclear andExcellent immunofluorescence nuclear and cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-23
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CREM 抗体 (C-2) is rated 4.7 out of 5 by 3.
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