Date published: 2025-9-6
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CREB1 Antibody (24H4B) - Western Blotting - Image 282502
CREB-1 抗体 (24H4B): sc-271. PC-3 (A), MCF7 (B), SK-BR-3 (C) 和 HL-60 (D) 核提取物中 CREB-1 表达的 Western 印迹分析.
CREB-1 抗体 (24H4B): sc-271. PC-3 (A), MCF7 (B), SK-BR-3 (C) 和 HL-60 (D) 核提取物中 CREB-1 表达的 Western 印迹分析.

CREB1 抗体 (24H4B): sc-271

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寫評論提問

说明书
  • CREB1抗体(24H4B)是小鼠单克隆IgG2b κ, 在105篇文献中引用，规格为200 µg/ml
  • 针对氨基酸254-327的抗原，在human的CREB-1的DNA结合和二聚化结构域内
  • 抗-CREB1 抗体 (24H4B) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 CREB-1A and CREB-1B
  • 抗CREB1抗体（24H4B）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271 X, 200 µg/0.1 ml)
  • 关于如何获取CREB1 (24H4B): sc-271的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CREB1 抗体 (24H4B)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外CREB抗体，包括ATF-4

CREB-1抗体（24H4B）是一种小鼠单克隆IgG2b kappa轻链抗体，针对人类CREB-1（也称为CREB和CREB1）的DNA结合和二聚化结构域内的氨基酸254-327进行工程改造，能够通过 ，能够通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）应用，在小鼠、大鼠和人类物种中可靠地检测CREB-1A和CREB-1B的同种型。CREB-1（cAMP反应元件结合蛋白1）是一种重要的转录因子，通过与目标基因启动子中的cAMP反应元件（CRE）结合来调节基因表达，从而调节代谢、神经元可塑性和存活等重要的细胞过程。CREB-1在丝氨酸133位被蛋白激酶A磷酸化后，会发生构象变化，从而促进与CREB结合蛋白（CBP）的相互作用，促进基本转录复合物的组装，并激活对细胞对激素和应激信号的反应至关重要的转录程序。这种调节机制强调了CREB-1通过与核共激活因子PGC-1相互作用而在新糖生成中的关键作用，以及参与II型糖尿病和扩张型心肌病的发病机制，突出了其在代谢调节和心血管健康中的重要性。有非结合形式和各种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CREB1 抗体 (24H4B)

sc-271
200 µg/ml
RMB2377.00

CREB1 抗体 (24H4B) X

sc-271 X
200 µg/0.1 ml
RMB2377.00

CREB1 抗体 (24H4B) AC

sc-271 AC
500 µg/ml, 25% agarose
RMB3129.00

CREB1 抗体 (24H4B) HRP

sc-271 HRP
200 µg/ml
RMB2377.00

CREB1 抗体 (24H4B) FITC

sc-271 FITC
200 µg/ml
RMB2482.00

CREB1 抗体 (24H4B) PE

sc-271 PE
200 µg/ml
RMB2580.00

CREB1 抗体 (24H4B) Alexa Fluor® 488

sc-271 AF488
200 µg/ml
RMB2685.00

CREB1 抗体 (24H4B) Alexa Fluor® 546

sc-271 AF546
200 µg/ml
RMB2685.00

CREB1 抗体 (24H4B) Alexa Fluor® 594

sc-271 AF594
200 µg/ml
RMB2685.00

CREB1 抗体 (24H4B) Alexa Fluor® 647

sc-271 AF647
200 µg/ml
RMB2685.00

CREB1 抗体 (24H4B) Alexa Fluor® 680

sc-271 AF680
200 µg/ml
RMB2685.00

CREB1 抗体 (24H4B) Alexa Fluor® 790

sc-271 AF790
200 µg/ml
RMB2685.00

CREB-1 Antibody (24H4B) 샘플을 받아보고 싶습니다.

Asked by: jeongwon
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다. 샘플을 받으려면 우리 회사 한국에 있는 distributor에게 물어보세요. 아래는 연락처입니다.이메일) [email protected] Website) http://korambiotech.com 이메일) [email protected] Website) http://www.essencemedical.com 다른 문제 있으시면 기술지원팀에 연락하셔서 전화:86 21-60936350 이메일: [email protected] 또는 온라인문의로 문의하시면 됩니다 Thank you for your question. For order question ,please contact our local distributor in Korea . Email: [email protected] Website: http://www.essencemedical.com Email: [email protected] Website: http://www.korambiotech.com If you have any other question ,please call us directly at (86 21) 6093-6350, email [email protected], or contact us by live chat.
Answered by: Technical Support 8
Date published: 2018-12-21

When should the TransCruz reagent sc-271 X be used instead of the standard form sc-271?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from IHC (Frozen)でワークします。マウス大脳皮質（4%PFA固定, 4℃、overnight）に染色性を認めました。線条体にはほとんどシグナルはありませんでした。上記固定条件では抗原賦活化処理(ph6.3クエン酸をした方が染色強度が高いです。
Date published: 2020-08-31
Rated 5 out of 5 by from Specific for CREB in WBI overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. CREB 24H4B was very specific to CREB in WB (dilution 1:200), as it recognised only GFP-CREB protein, but not GFP-ATF1 or GFP-CREM.
Date published: 2018-08-23
Rated 2 out of 5 by from does not work for western in MDA-MB-231 cellsThis CREB-1 antibody does not work for western in MDA-MB-231 cells
Date published: 2018-02-02
Rated 1 out of 5 by from Doesn't work with zebrafish proteinsI tried this antibody with proteins from zebrafish testicle, using two different concentrations in WB. But it didn't work with these proteins.
Date published: 2018-01-24
Rated 1 out of 5 by from Doesn't work with zebrafish proteinsI tried this antibody with proteins from zebrafish testicle, using two different concentrations in WB. But it didn't work with these proteins.
Date published: 2018-01-16
Rated 5 out of 5 by from Superb antibody!CREB has multiple isoforms and often it is difficult to find an antibody which is isoform specific. SCBT anti-CREB1 (CREB-1(24H4B),Sc271) antibody is very specific in immunoblotting and does not recognize the CREB-2 isoform (See my blot). The CREB-1 antibody recognized approximately 40kDa protein in HEK293 (human) cells which is also picked up by an anti-pCREB antibody (top band, see my blot) suggesting further the specificity. So, highly recommend this antibody.
Date published: 2017-09-07
Rated 4 out of 5 by from 效果还可以之前用过货号为CREB1 SC-37715得试用装，感觉还可以，条带单一，没有杂带，就是抗体稀释比例要小。
Date published: 2017-03-13
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of CREB-1 expression in PC-3, MCF7, SK-BR-3 and HL-60 nuclear extracts.
Date published: 2017-01-20
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CREB1 抗体 (24H4B) is rated 4.1 out of 5 by 14.
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