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creatine kinase-M/B Antibody (CK1) - Western Blotting - Image 17572
肌酸激酶-M/B 抗体 (CK1): sc-56896. SH-SY5Y (A) 和 HeLa (B) 全细胞裂解液中肌酸激酶-M/B 表达的 Western 印迹分析.
creatine kinase-M/B Antibody (CK1) - Western Blotting - Image 17572
肌酸激酶-M/B 抗体 (CK1): sc-56896. SH-SY5Y (A) 和 HeLa (B) 全细胞裂解液中肌酸激酶-M/B 表达的 Western 印迹分析.
肌酸激酶-M/B 抗体 (CK1): sc-56896. SH-SY5Y (A) 和 HeLa (B) 全细胞裂解液中肌酸激酶-M/B 表达的 Western 印迹分析.

creatine kinase-M/B 抗体 (CK1): sc-56896

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说明书
  • creatine kinase-M/B 抗体 CK1 是小鼠单克隆 IgG1，提供 100 µg/ml
  • 针对全长天然二聚体creatine kinase-M/B dimer（来源于human）
  • 推荐用于 human 来源的creatine kinase-M/B WB, IP 和 ELISA检测
  • 目前，我们还没有完成creatine kinase-M/B 抗体 (CK1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    此外 Creatine Kinase 抗体包括 creatine kinase-B 和 creatine kinase-M

    肌酸激酶（CKs）是一个大的同工酶家族，它们在亚细胞区室内调节ATP的水平。通过在肌酸和腺嘌呤核苷酸之间转移磷酸盐，它们在有波动能量需求的位点提供ATP。肌酸激酶为驱动许多细胞系统的正常功能提供了必要的磷酸水解能量，这些细胞系统包括肌肉、发电细胞、视网膜感光细胞、脑细胞、肾脏、盐腺、子宫肌层、胎盘、胰腺、胸腺、甲状腺、肠上皮细胞、内皮细胞、软骨和骨细胞、巨噬细胞、血小板以及肿瘤和癌细胞。人细胞质肌酸激酶-B，也被称为CK-B和BCK，是肌酸激酶的一种由381个氨基酸组成的脑组织特异性亚型。人细胞质肌酸激酶-M（CK-M，MCK）是肌酸激酶的一种肌肉组织特异性亚型。人细胞质肌酸激酶-Mi（Mi-CK，MtCK）是肌酸激酶的一种由416个氨基酸组成的线粒体特异性亚型。细胞溶质肌酸激酶在Ca2+泵的能量调节和维持Ca2+稳态中起着重要作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    creatine kinase-M/B 抗体 (CK1) 参考文献:

    1. 使用肌酸激酶M亚基活性的免疫抑制测定血清中肌酸激酶同工酶MB的活性。心肌梗死中的活性动力学和诊断意义。  |  Neumeier, D., et al. 1976. Clin Chim Acta. 73: 445-51. PMID: 1000863
    2. 多发性硬化症正常白质中肌酸激酶B活性降低。  |  Steen, C., et al. 2010. PLoS One. 5: e10811. PMID: 20520825
    3. 肌酸激酶B中的异天冬氨酸形成与酶活性丧失有关；异天冬氨酸积累与PIMT基因敲除小鼠神经功能障碍之间的联系。  |  Dimitrijevic, A., et al. 2014. PLoS One. 9: e100622. PMID: 24955845
    4. 人肌酸激酶：B亚基的cDNA克隆的分离和序列分析，亚基特异性探针的开发以及基因拷贝数的测定。  |  Villarreal-Levy, G., et al. 1987. Biochem Biophys Res Commun. 144: 1116-27. PMID: 3034271
    5. 人类肌酸激酶 B 基因的结构和表达。  |  Mariman, EC., et al. 1987. Genomics. 1: 126-37. PMID: 3692484
    6. 氮质血症、阻塞性尿路疾病或前列腺癌或膀胱癌患者血清中肌酸激酶B同工酶的放射免疫测定。  |  Homburger, HA., et al. 1980. Clin Chem. 26: 1821-4. PMID: 6159995
    7. 急性脑血管疾病中血清肌酸激酶B的浓度。  |  Pfeiffer, FE., et al. 1984. Arch Neurol. 41: 1175-8. PMID: 6487101
    8. 疑似心肌梗塞病例中血清肌酸激酶和肌酸激酶B亚基的活性。  |  Gerhardt, W., et al. 1982. Clin Chem. 28: 277-83. PMID: 7055948
    9. 不同的调控序列控制直接注射骨骼肌和心肌中的肌酸激酶-M基因表达。  |  Vincent, CK., et al. 1993. Mol Cell Biol. 13: 1264-72. PMID: 8423791
    10. 早期和晚期精子发生的生化标记：人精子中乳酸脱氢酶-X和肌酸激酶-M同工型浓度之间的关系。  |  Lalwani, S., et al. 1996. Mol Reprod Dev. 43: 495-502. PMID: 9052941

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    产品名称产品编号规格价格数量收藏夹

    creatine kinase-M/B 抗体 (CK1)

    sc-56896
    100 µg/ml
    		RMB2377.00

    I am doing sandwich ELISA and I would like to know which epitopes recognice this antibody as I need to find a pair of antibodies that detect different epitopes and can be used together as capture and detection! Thanks

    Asked by: Marian_Bionos
    Thanks for your question Unfortunately this antibody has not yet been epitope mapped, so we do not have this information for this clone. It was raised against full length native creatine kinase-M/B dimer of human origin.
    Answered by: Tech Service
    Date published: 2017-03-31

    What is the molecular weight of creatine kinase-M/B ?

    Asked by: TinTin
    Thank you for your question. creatine kinase-M/B has an expected molecular weight of approximately 43 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 4 out of 5 by from Nice bands seen in WB using SHNice bands seen in WB using SH-SY5Y and HeLa whole cell lysates. -SCBT QC
    Date published: 2014-12-14
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    creatine kinase-M/B 抗体 (CK1) is rated 4.0 out of 5 by 1.
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