Date published: 2025-9-7
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creatine kinase-B Antibody (B-9) - Western Blotting - Image 414432
肌酸激酶-B 抗体（B-9）：sc-373686。Western blot 分析 HeLa（A）、K-562（B）和 HCT-116（C）细胞裂解液中肌酸激酶-B 的表达。检测试剂：m-IgGκ BP-HRP：sc-516102。
肌酸激酶-B 抗体（B-9）：sc-373686。Western blot 分析 HeLa（A）、K-562（B）和 HCT-116（C）细胞裂解液中肌酸激酶-B 的表达。检测试剂：m-IgGκ BP-HRP：sc-516102。

creatine kinase-B 抗体 (B-9): sc-373686

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说明书
  • creatine kinase-B 抗体 B-9 是小鼠单克隆 IgG3 κ，creatine kinase-B抗体, 在4篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的creatine kinase-B的N-terminus的氨基酸3-30之间
  • 推荐用于 mouse, rat 和 human 来源的creatine kinase-B chain WB, IP, IF 和 ELISA检测
  • 关于如何获取creatine kinase-B (B-9): sc-373686的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是creatine kinase-B Antibody (B-9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与creatine kinase-B Antibody (B-9) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

附加Creatine Kinase抗体，包括creatine kinase-B和creatine kinase-M

肌酸激酶B抗体(B-9)是一种IgG3 κ小鼠单克隆肌酸激酶B抗体(也称为肌酸激酶B抗体)，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的肌酸激酶B蛋白。肌酸激酶B抗体(B-9)可作为非偶联的抗肌酸激酶B抗体使用。肌酸激酶(CKs)是一个庞大的同工酶家族，可调节亚细胞区室中的ATP水平，通过在肌酸和腺嘌呤核苷酸之间转移磷酸盐，在能量需求波动的位点提供ATP。肌酸激酶为驱动许多细胞系统的正常功能提供了磷酸盐水解所需的能量，这些细胞系统包括肌肉、电细胞、视网膜光感受器细胞、脑细胞、肾脏、盐腺、子宫肌层、胎盘、胰腺、胸腺、甲状腺、肠上皮细胞、内皮细胞、软骨和骨细胞、巨噬细胞、血小板以及肿瘤和癌细胞。人细胞质肌酸激酶B，也称为CK-B和BCK，是一种381个氨基酸的脑组织特异性肌酸激酶异构体。人细胞质肌酸激酶-肌肉(CK-M，MCK)是肌酸激酶的一种肌肉组织特异性异构体。人细胞质肌酸激酶-Mi(Mi-CK，MtCK)是一种416个氨基酸的线粒体特异性肌酸激酶异构体。胞质肌酸激酶在Ca2+泵的能量调节和Ca2+稳态的维持中起重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

creatine kinase-B 抗体 (B-9) 参考文献:

  1. 人类肌酸激酶 B 基因的完整核苷酸序列。  |  Mariman, EC., et al. 1989. Nucleic Acids Res. 17: 6385. PMID: 2771648
  2. 人类线粒体肌酸激酶基因和 cDNA 的分离与鉴定。  |  Haas, RC., et al. 1989. J Biol Chem. 264: 2890-7. PMID: 2914937
  3. 人类肌酸激酶 M 基因的 cDNA 克隆和 19q13 图谱绘制。  |  Nigro, JM., et al. 1987. Am J Hum Genet. 40: 115-25. PMID: 3031982
  4. 人类肌酸激酶 B 基因的结构和表达。  |  Mariman, EC., et al. 1987. Genomics. 1: 126-37. PMID: 3692484
  5. 非肌肉组织和细胞中的肌酸激酶。  |  Wallimann, T. and Hemmer, W. 1994. Mol Cell Biochem. 133-134: 193-220. PMID: 7808454
  6. 肌酸激酶（CK）的一些新方面：细胞和线粒体生物能和生理学的分区, 结构, 功能和调节。  |  Wallimann, T., et al. 1998. Biofactors. 8: 229-34. PMID: 9914824

订购信息

产品名称产品编号规格价格数量收藏夹

creatine kinase-B 抗体 (B-9)

sc-373686
200 µg/ml
RMB2377.00

creatine kinase-B (B-9): m-IgGκ BP-HRP 套装

sc-552016
200 µg Ab; 40 µg BP
RMB2662.00

creatine kinase-B (B-9) 中和勝肽

sc-373686 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-373686 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from GoodA band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Date published: 2020-06-26
Rated 5 out of 5 by from works finewe were given a sample to try. it works just fine. It could detect as least as 5ug of WCL loaded. I re-used the diluted antibody up to 5-8 times (stored the diluted aliquot at -20oC). For WB i used TBST, and for blocking I used 5% milk in TBST.
Date published: 2018-08-27
Rated 5 out of 5 by from Strong band in extract from human Hela cellsStrong band with no background in Western blot using whole cell lysates from human HeLa cells.
Date published: 2018-01-29
Rated 5 out of 5 by from Good antibody for WBThis creatine kinase B antibody works well in mouse brain and mouse hippocampus. Strong signal and clean background.
Date published: 2017-11-03
Rated 5 out of 5 by from Excellent antibody.Strong band without unspecific bands and little background in WB with brain extracts
Date published: 2017-09-20
Rated 5 out of 5 by from Great monoclonal antibodyNice strong band observed with creatine kinase-B Antibody (B-9) with little to no background. IF stain was robust and specific
Date published: 2017-02-09
Rated 4 out of 5 by from Strong band detected in WB using mouse brainStrong band detected in WB using mouse brain tissue extract. -SCBT QC
Date published: 2015-06-15
Rated 4 out of 5 by from Robust bands seen in WB using NCIRobust bands seen in WB using NCI-H1688 and SH-SY5Y whole cell lysates. -SCBT QC
Date published: 2015-01-08
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creatine kinase-B 抗体 (B-9) is rated 4.8 out of 5 by 10.
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