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creatine kinase-M Antibody (MM-2) - Western Blotting - Image 358005
肌酸激酶-M 抗体 (MM-2): sc-69848. HeLa (A) 和 SH-SY5Y (B) 全细胞裂解液中肌酸激酶-M 表达的 Western 印迹分析.
肌酸激酶-M 抗体 (MM-2): sc-69848. HeLa (A) 和 SH-SY5Y (B) 全细胞裂解液中肌酸激酶-M 表达的 Western 印迹分析.

creatine kinase-M 抗体 (MM-2): sc-69848

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寫評論提問

说明书
  • creatine kinase-M抗体(MM-2)是小鼠单克隆IgG1 κ, 在1篇文献中引用，规格为200 µg/ml
  • 免疫human物种的creatine kinase-M
  • 推荐用于 mouse, rat 和 human 来源的creatine kinase-M WB, IP 和 IF检测
  • m-IgG Fc BP-HRP是creatine kinase-M Antibody (MM-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与creatine kinase-M Antibody (MM-2) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

附加Creatine Kinase抗体，包括creatine kinase-B和creatine kinase-M

肌酸激酶（CKs）是一个大的同工酶家族，它们在亚细胞区室内调节ATP的水平。通过在肌酸和腺嘌呤核苷酸之间转移磷酸盐，它们在有波动能量需求的位点提供ATP。肌酸激酶为驱动许多细胞系统的正常功能提供了必要的磷酸水解能量，这些细胞系统包括肌肉、发电细胞、视网膜感光细胞、脑细胞、肾脏、盐腺、子宫肌层、胎盘、胰腺、胸腺、甲状腺、肠上皮细胞、内皮细胞、软骨和骨细胞、巨噬细胞、血小板以及肿瘤和癌细胞。人细胞质肌酸激酶-B，也被称为CK-B和BCK，是肌酸激酶的一种由381个氨基酸组成的脑组织特异性亚型。人细胞质肌酸激酶-M（CK-M，MCK）是肌酸激酶的一种肌肉组织特异性亚型。人细胞质肌酸激酶-Mi（Mi-CK，MtCK）是肌酸激酶的一种由416个氨基酸组成的线粒体特异性亚型。细胞溶质肌酸激酶在Ca2+泵的能量调节和维持Ca2+稳态中起着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

creatine kinase-M 抗体 (MM-2) 参考文献:

  1. 肌酸激酶M亚型缺陷是肌强直性营养不良的病因，评估肌酸激酶M亚型缺陷并鉴定两种新的CKMM多态性。  |  Bailly, J., et al. 1991. Hum Genet. 86: 457-62. PMID: 2016086
  2. 肌酸激酶（CKMM）与19号染色体上的肌营养不良症密切相关。  |  Yamaoka, LH., et al. 1990. Neurology. 40: 222-6. PMID: 2300239
  3. 人类精子中肌酸激酶B型和M型同工酶合成的精子发生相关变化。  |  Huszar, G. and Vigue, L. 1990. Mol Reprod Dev. 25: 258-62. PMID: 2331374
  4. 注射M型同种异体蛋白或富含M型肌酸激酶信息的聚（A+）-RNA后，分化鸡心脏细胞中出现M型肌酸激酶附带的M带。  |  Schäfer, B., et al. 1985. Basic Res Cardiol. 80 Suppl 2: 129-33. PMID: 2415105
  5. 马肌酸激酶肌肉（CK-M）基因的分子特征和表达分析。  |  Do, KT., et al. 2015. Asian-Australas J Anim Sci. 28: 1680-5. PMID: 26580434
  6. 肌强直性营养不良与肌型肌酸激酶（CKMM）基因密切相关。  |  Brunner, HG., et al. 1989. Hum Genet. 81: 308-10. PMID: 2703233
  7. 肌酸激酶肌肉型基因位点（CKMM，19号染色体）的NcoI RFLP。  |  Coerwinkel-Driessen, M., et al. 1988. Nucleic Acids Res. 16: 8743. PMID: 2901730
  8. 肌酸激酶肌肉型与饱和脂肪酸和/或单不饱和脂肪酸的磷脂酸有特定的相互作用。  |  Hoshino, F., et al. 2019. Biochem Biophys Res Commun. 513: 1035-1040. PMID: 31010675
  9. 两全其美：在急诊科取消肌酸激酶-肌肉/脑（CK-MB）测试，既降低成本，又不会错过临床诊断。  |  Sahadeo, PA., et al. 2021. Cureus. 13: e15150. PMID: 34164248
  10. 肌酸激酶M和B的mRNA在跑步机训练的大鼠骨骼肌中的表达。  |  Apple, FS. and Billadello, JJ. 1994. Life Sci. 55: 585-92. PMID: 8046994

订购信息

产品名称产品编号规格价格数量收藏夹

creatine kinase-M 抗体 (MM-2)

sc-69848
200 µg/ml
RMB2377.00

creatine kinase-M (MM-2): m-IgG Fc BP-HRP 套装

sc-539244
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for creatine kinase-M (MM-2): sc-69848 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-69848.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-20
Rated 4 out of 5 by from Band seen in WB using human skeletal muscleBand seen in WB using human skeletal muscle tissue extract. -SCBT QC
Date published: 2015-01-24
Rated 4 out of 5 by from Band seen in WB using mouse heart and ratBand seen in WB using mouse heart and rat skeletal muscle tissue extracts. -SCBT QC
Date published: 2013-03-08
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creatine kinase-M 抗体 (MM-2) is rated 4.0 out of 5 by 3.
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