Date published: 2025-9-7
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creatine kinase-M Antibody (G-9) - Western Blotting - Image 355609
肌酸激酶-M 抗体 (G-9): sc-365046. 人心脏 (A) 和小鼠骨骼肌 (B) 组织提取物中肌酸激酶-M 表达的 Western 印迹分析.
肌酸激酶-M 抗体 (G-9): sc-365046. 人心脏 (A) 和小鼠骨骼肌 (B) 组织提取物中肌酸激酶-M 表达的 Western 印迹分析.

creatine kinase-M 抗体 (G-9): sc-365046

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说明书
  • creatine kinase-M 抗体 G-9 是小鼠单克隆 IgG1 κ，creatine kinase-M抗体, 在6篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的creatine kinase-M的N-terminus附近的氨基酸9-27之间
  • creatine kinase-M 抗体 (G-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 creatine kinase-M chain; 也和以下物种反应，包括： equine
  • 抗creatine kinase-M抗体（G-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取creatine kinase-M (G-9): sc-365046的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 creatine kinase-M 抗体 (G-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与creatine kinase-M 抗体 (G-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Creatine Kinase 抗体 包括 creatine kinase-M

肌酸激酶-M抗体（G-9）是一种IgG1κ小鼠单克隆肌酸激酶-M抗体（也称为CKM抗体或CKMM抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的肌酸激酶-M蛋白。肌酸激酶-M抗体（G-9）既可作为非偶联的抗肌酸激酶-M抗体形式，也可作为多种偶联形式的抗肌酸激酶-M抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌酸激酶（CK）是一个大型的同工酶家族，调节亚细胞区室中的ATP水平，通过在肌酸和腺嘌呤核苷酸之间转移磷酸盐，在能量需求波动的位点提供ATP。肌酸激酶提供驱动许多细胞系统正常功能所需的磷酸水解能量，这些细胞系统包括肌肉、电细胞、视网膜感光细胞、脑细胞、肾脏、盐腺、子宫肌层、胎盘、胰腺、胸腺、甲状腺、肠上皮细胞、内皮细胞、软骨和骨细胞、巨噬细胞、血小板、肿瘤和癌症细胞。人细胞质肌酸激酶-B，也称为CK-B和BCK，是一种381个氨基酸的肌酸激酶脑组织特异性亚型。人肌浆细胞肌酸激酶-M（CK-M，MCK）是肌酸激酶的一种肌肉组织特异性亚型。人细胞质肌酸激酶-Mi（Mi-CK，MtCK）是肌酸激酶的一种416个氨基酸的线粒体特异性亚型。细胞溶质肌酸激酶在Ca2+泵的能量调节和维持Ca2+稳态中起着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

creatine kinase-M 抗体 (G-9) 参考文献:

  1. 肌酸激酶M亚型缺陷是肌强直性营养不良的病因，评估肌酸激酶M亚型缺陷并鉴定两种新的CKMM多态性。  |  Bailly, J., et al. 1991. Hum Genet. 86: 457-62. PMID: 2016086
  2. 非心脏手术后肌钙蛋白和肌酸激酶肌肉和脑同工酶测量的预后价值：系统评价和荟萃分析。  |  Levy, M., et al. 2011. Anesthesiology. 114: 796-806. PMID: 21336095
  3. CK-MM干扰血清中CK-MB的免疫酶法定量。  |  Panteghini, M., et al. 1989. Clin Chem. 35: 901-2. PMID: 2656004
  4. 人类肌酸激酶 B 基因的完整核苷酸序列。  |  Mariman, EC., et al. 1989. Nucleic Acids Res. 17: 6385. PMID: 2771648
  5. 肌酸激酶肌肉型基因位点（CKMM，19号染色体）的NcoI RFLP。  |  Coerwinkel-Driessen, M., et al. 1988. Nucleic Acids Res. 16: 8743. PMID: 2901730
  6. 人类线粒体肌酸激酶基因和 cDNA 的分离与鉴定。  |  Haas, RC., et al. 1989. J Biol Chem. 264: 2890-7. PMID: 2914937
  7. 评估两种用于CK-MM同工型的商业测试试剂盒，以早期识别急性心肌梗塞。  |  Shah, VD., et al. 1989. Clin Chem. 35: 493-4. PMID: 2920421
  8. 人类肌酸激酶 M 基因的 cDNA 克隆和 19q13 图谱绘制。  |  Nigro, JM., et al. 1987. Am J Hum Genet. 40: 115-25. PMID: 3031982
  9. 肌酸激酶肌肉型与饱和脂肪酸和/或单不饱和脂肪酸的磷脂酸有特定的相互作用。  |  Hoshino, F., et al. 2019. Biochem Biophys Res Commun. 513: 1035-1040. PMID: 31010675
  10. 人类肌酸激酶 B 基因的结构和表达。  |  Mariman, EC., et al. 1987. Genomics. 1: 126-37. PMID: 3692484
  11. 非肌肉组织和细胞中的肌酸激酶。  |  Wallimann, T. and Hemmer, W. 1994. Mol Cell Biochem. 133-134: 193-220. PMID: 7808454
  12. 肌酸激酶（CK）的一些新方面：细胞和线粒体生物能和生理学的分区, 结构, 功能和调节。  |  Wallimann, T., et al. 1998. Biofactors. 8: 229-34. PMID: 9914824

订购信息

产品名称产品编号规格价格数量收藏夹

creatine kinase-M 抗体 (G-9)

sc-365046
200 µg/ml
RMB2377.00

creatine kinase-M (G-9): m-IgG Fc BP-HRP 套装

sc-529305
200 µg Ab; 10 µg BP
RMB2662.00

creatine kinase-M (G-9): m-IgGκ BP-HRP 套装

sc-522127
200 µg Ab, 40 µg BP
RMB2662.00

creatine kinase-M (G-9): m-IgG1 BP-HRP 套装

sc-543442
200 µg Ab; 20 µg BP
RMB2662.00

creatine kinase-M 抗体 (G-9) AC

sc-365046 AC
500 µg/ml, 25% agarose
RMB3129.00

creatine kinase-M 抗体 (G-9) HRP

sc-365046 HRP
200 µg/ml
RMB2377.00

creatine kinase-M 抗体 (G-9) FITC

sc-365046 FITC
200 µg/ml
RMB2482.00

creatine kinase-M 抗体 (G-9) PE

sc-365046 PE
200 µg/ml
RMB2580.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 488

sc-365046 AF488
200 µg/ml
RMB2685.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 546

sc-365046 AF546
200 µg/ml
RMB2685.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 594

sc-365046 AF594
200 µg/ml
RMB2685.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 647

sc-365046 AF647
200 µg/ml
RMB2685.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 680

sc-365046 AF680
200 µg/ml
RMB2685.00

creatine kinase-M 抗体 (G-9) Alexa Fluor® 790

sc-365046 AF790
200 µg/ml
RMB2685.00

creatine kinase-M (G-9) 中和勝肽

sc-365046 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-365046 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from worked well for Western blot of heart lysateSingle band at the expected molecular weight in a western blot with mouse heart lysate.
Date published: 2017-09-20
Rated 5 out of 5 by from good dataIt is well known that creatine kinase is a large family of isoenzymes that regulate ATP levels in subcellular compartments, where they provide ATP sites for phosphotransfer energy requirements for the transfer of phosphate between creatine and adenine nucleotides . Overall, the product characteristics of good quality of the experiment to provide better data.
Date published: 2017-02-02
Rated 4 out of 5 by from Good antibody for western blottingWe received this antibody on a trial basis from our sales rep Korey Griffin. It was used at a 1:1000 dilution for western blotting of L6 cell lysates to confirm differentiation of myoblasts to myotubes. Bands were seen at the expected molecular weight of 43kD.
Date published: 2016-11-04
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Robust bands seen in WB using human skeletalRobust bands seen in WB using human skeletal muscle and human heart tissue extracts. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-01
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creatine kinase-M 抗体 (G-9) is rated 4.5 out of 5 by 6.
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