Date published: 2025-9-9
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Cox-2 Antibody (D-12) - Western Blotting - Image 414687
Cox-2抗体（D-12）：sc-166475。近红外Western blot分析Cox-2在未转染的293T细胞裂解液（sc-117752，A）、转染人Cox-2的293T细胞裂解液（sc-113099，B）及C2C12细胞裂解液（C）中的表达。检测试剂：m-IgG1 BP-CFL 680：sc-533665。用UltraCruz®阻断试剂阻断：sc-516214。
Cox-2抗体（D-12）：sc-166475。近红外Western blot分析Cox-2在未转染的293T细胞裂解液（sc-117752，A）、转染人Cox-2的293T细胞裂解液（sc-113099，B）及C2C12细胞裂解液（C）中的表达。检测试剂：m-IgG1 BP-CFL 680：sc-533665。用UltraCruz®阻断试剂阻断：sc-516214。

Cox-2 抗体 (D-12): sc-166475

4.6(13)
寫評論提問

说明书
  • Cox-2抗体(D-12)是小鼠单克隆IgG1 κ, 在68篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Cox-2的C-terminus的氨基酸586-604之间
  • 抗-Cox-2 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cox-2
  • 抗Cox-2抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cox-2 (D-12): sc-166475的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Cox-2 Antibody (D-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Cox-2 Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Cox抗体，包括Cox-1和Cox-2

Cox-2抗体（D-12）是一种IgG1 κ小鼠单克隆Cox-2抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的Cox-2。Cox-2抗体（D-12）既可作为非共轭抗Cox-2抗体形式存在，也可作为多种共轭形式的抗Cox-2抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。前列腺素是一组多样化的自分泌和旁分泌激素，介导许多细胞和生理过程。前列腺素H2（PGH2）是形成前列腺素过程中的中间分子。环氧化酶-1（Cox-1）和环氧化酶-2（Cox-2）是前列腺素合酶，催化花生四烯酸（AA）形成PGH2。Cox-1和Cox-2是前列腺素内过氧化物合酶（PTGS）的同工酶。Cox-1在大多数组织中均有表达，被认为具有一般的"管家"功能。Cox-2在响应促炎刺激的迁移细胞中有效诱导，被认为是炎症的重要介质。这两种酶都是非甾体抗炎药物（NSAIDs）的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Cox-2 抗体 (D-12)

sc-166475
200 µg/ml
RMB2377.00

Cox-2 (D-12): m-IgG Fc BP-HRP 套装

sc-528975
200 µg Ab; 10 µg BP
RMB2662.00

Cox-2 (D-12): m-IgGκ BP-HRP 套装

sc-521623
200 µg Ab, 40 µg BP
RMB2662.00

Cox-2 (D-12): m-IgG1 BP-HRP 套装

sc-543248
200 µg Ab; 20 µg BP
RMB2662.00

Cox-2 抗体 (D-12) AC

sc-166475 AC
500 µg/ml, 25% agarose
RMB3129.00

Cox-2 抗体 (D-12) HRP

sc-166475 HRP
200 µg/ml
RMB2377.00

Cox-2 抗体 (D-12) FITC

sc-166475 FITC
200 µg/ml
RMB2482.00

Cox-2 抗体 (D-12) PE

sc-166475 PE
200 µg/ml
RMB2580.00

Cox-2 抗体 (D-12) Alexa Fluor® 488

sc-166475 AF488
200 µg/ml
RMB2685.00

Cox-2 抗体 (D-12) Alexa Fluor® 546

sc-166475 AF546
200 µg/ml
RMB2685.00

Cox-2 抗体 (D-12) Alexa Fluor® 594

sc-166475 AF594
200 µg/ml
RMB2685.00

Cox-2 抗体 (D-12) Alexa Fluor® 647

sc-166475 AF647
200 µg/ml
RMB2685.00

Cox-2 抗体 (D-12) Alexa Fluor® 680

sc-166475 AF680
200 µg/ml
RMB2685.00

Cox-2 抗体 (D-12) Alexa Fluor® 790

sc-166475 AF790
200 µg/ml
RMB2685.00

Cox-2 (D-12) 中和勝肽

sc-166475 P
100 µg/0.5 ml
RMB511.00

a549cell에서 cox-2(sc-166475)항체를 붙여서 westernblot 실험을 했는데 분자량이 70kda 보다 밑인 40대kda에서 검출되면 잘못된건가요? 아니면 잘된건가요?

Asked by: pjk020202
Thank you for your question. The molecular weight of Cox-2 is 70 - 72 kDa, and the 40 kDa band might be non-specific.
Answered by: Ling Chen
Date published: 2025-08-13

Does Cox-2 (D-12) react with canine samples?

Asked by: sweetums
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

How should the blocking peptide sc-166475 P be used?

Asked by: sweetums
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the Cox-2 antibody sc-166475 with it prior to application. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 13
Date published: 2017-01-16
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Rated 5 out of 5 by from Antibody works with mouse microglia SIM A9 cellsThe COX-2 (D-12) antibody works very well in LPS-stimulated mouse microglia SIM A9 cells.
Date published: 2021-06-24
Rated 3 out of 5 by from Worked for WB analysisTried 1:500 dilution for WB analysis - worked for canine bladder cancer cells but not human bladder cancer cells
Date published: 2018-06-19
Rated 5 out of 5 by from so sensitiveI use 1:500 to do WB experiment. This antibody specifically completes the experiments and displays the target band.
Date published: 2017-11-01
Rated 5 out of 5 by from Very GoodPositive detection of Cox-2 protein from RAW264.7 cells was using this antibody by western blotting.
Date published: 2017-07-27
Rated 5 out of 5 by from very wellWB : 1/500 on mouse monocyte cell line, the experiment went very well and the result was satisfactory.
Date published: 2017-04-05
Rated 5 out of 5 by from Very goodVery good Cox-2 antibody for WB ,specific band, I suggest dilution this antibody at 1:800
Date published: 2017-03-11
Rated 5 out of 5 by from Great productVery strong IHC and IF staining data; also great WB data
Date published: 2017-01-26
Rated 5 out of 5 by from A good choice for stainingThis antibody is a great choice for IF and IHC and works wonderfully in mouse, rat, or human. It will also provide strong WB bands. It has been published with more than 10 times.
Date published: 2017-01-18
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Cox-2 抗体 (D-12) is rated 4.6 out of 5 by 13.
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