Date published: 2025-9-7

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Clathrin Light Chain B/CLTB 抗体 (G-7): sc-376414

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说明书
  • Clathrin Light Chain B/CLTB抗体(G-7)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列41-100位于human物种的Clathrin HC的内部区域内
  • Clathrin Light Chain B/CLTB 抗体 (G-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Clathrin LCB
  • 抗Clathrin Light Chain B/CLTB抗体(G-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Clathrin Light Chain B/CLTB (G-7): sc-376414免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Clathrin Light Chain B/CLTB 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Clathrin Light Chain B/CLTB 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

网格蛋白轻链B/CLTB抗体(G-7)是一种IgG1 κ小鼠单克隆网格蛋白LCB抗体(也称为网格蛋白LCB抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的网格蛋白LCB。网格蛋白轻链B/CLTB抗体(G-7)既可作为非偶联形式的抗网格蛋白LCB抗体提供,也可作为多种偶联形式的抗网格蛋白LCB抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。网格蛋白是源自质膜和反式高尔基体网络的凹坑和囊泡中的主要胞质被膜蛋白。在受体介导的内吞作用中,受体蛋白被网格蛋白包被的囊泡吞噬。网格蛋白由三个重链和三个轻链非共价结合而成,形成三脚架结构。网格蛋白轻链调节三脚架在细胞内膜上的自我组装。网格蛋白轻链亚基(LCA和LCB)有助于调节受体介导的内吞作用和细胞器生物发生过程中包被囊泡的形成以分拣蛋白质。尽管LCA和LCB由仅共享60%同源性的两个不同基因编码,但它们具有某些共同特征。LCA和LCB都经历了mRNA选择性剪接,产生了组织特异性的异构体。此外,在大脑中,LCA和LCB包含插入序列,这些序列形成了更高分子量的异构体。这些序列插入在LCA和LCB的相似细胞质结构域编码区中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Clathrin Light Chain B/CLTB 抗体 (G-7) 参考文献:

  1. Clathrin 轻链 b 能够有效地影响微管的一种主要蛋白磷酸酶(MT-PP1)。  |  Hiraga, A., et al. 2006. FEBS Lett. 580: 1425-30. PMID: 16460735
  2. 神经元特异性表达高分子量凝集素轻链  |  Wong, DH., et al. 1990. J Neurosci. 10: 3025-31. PMID: 2204686
  3. clathrin轻链LCb的优势与调节分泌途径的存在相关。  |  Acton, SL. and Brodsky, FM. 1990. J Cell Biol. 111: 1419-26. PMID: 2211818
  4. 包膜囊泡的结构和组装。  |  Pearse, BM. and Crowther, RA. 1987. Annu Rev Biophys Biophys Chem. 16: 49-68. PMID: 2885011
  5. Clathrin 和包被囊泡。  |  Pearse, BM. 1987. EMBO J. 6: 2507-12. PMID: 2890519
  6. 识别蛋白酶体轻链LCb的磷酸化位点。  |  Hill, BL., et al. 1988. J Biol Chem. 263: 5499-501. PMID: 3128543
  7. 人类凝集素轻链的结构。三种哺乳动物轻链多态性的一致性。  |  Jackson, AP. and Parham, P. 1988. J Biol Chem. 263: 16688-95. PMID: 3267234
  8. 蛋白酶体轻链LCA和LCB相似、多态且具有重复的七元基序。  |  Kirchhausen, T., et al. 1987. Science. 236: 320-4. PMID: 3563513
  9. 人类凝集素轻链基因(CLTA 和 CLTB)的染色体位置和一些结构特征。  |  Ponnambalam, S., et al. 1994. Genomics. 24: 440-4. PMID: 7713494
  10. 钙调蛋白与凝集素包被囊泡, 三叉小体和轻链的相互作用。结合位点的定域。  |  Pley, UM., et al. 1995. J Biol Chem. 270: 2395-402. PMID: 7836475
  11. 凝集素轻链在阿尔茨海默病病理学中的参与。  |  Nakamura, Y., et al. 1994. Acta Neuropathol. 87: 23-31. PMID: 8140893
  12. 通过转染和基因干扰改变 clathrin 轻链的表达。  |  Acton, SL., et al. 1993. Mol Biol Cell. 4: 647-60. PMID: 8374173

订购信息

产品名称产品编号规格价格数量收藏夹

Clathrin Light Chain B/CLTB 抗体 (G-7)

sc-376414
200 µg/ml
RMB2377.00

Clathrin Light Chain B/CLTB (G-7): m-IgG Fc BP-HRP 套装

sc-527534
200 µg Ab; 10 µg BP
RMB2662.00

Clathrin Light Chain B/CLTB (G-7): m-IgG1 BP-HRP 套装

sc-532907
200 µg Ab; 20 µg BP
RMB2662.00

Clathrin Light Chain B/CLTB 抗体 (G-7) AC

sc-376414 AC
500 µg/ml, 25% agarose
RMB3129.00

Clathrin Light Chain B/CLTB 抗体 (G-7) HRP

sc-376414 HRP
200 µg/ml
RMB2377.00

Clathrin Light Chain B/CLTB 抗体 (G-7) FITC

sc-376414 FITC
200 µg/ml
RMB2482.00

Clathrin Light Chain B/CLTB 抗体 (G-7) PE

sc-376414 PE
200 µg/ml
RMB2580.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 488

sc-376414 AF488
200 µg/ml
RMB2685.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 546

sc-376414 AF546
200 µg/ml
RMB2685.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 594

sc-376414 AF594
200 µg/ml
RMB2685.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 647

sc-376414 AF647
200 µg/ml
RMB2685.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 680

sc-376414 AF680
200 µg/ml
RMB2685.00

Clathrin Light Chain B/CLTB 抗体 (G-7) Alexa Fluor® 790

sc-376414 AF790
200 µg/ml
RMB2685.00

Does this antibody block clathrin mediated endocytosis?

Asked by: cnh14
Thank you for your question. This antibody has not been tested for blocking applications, so we do not have this information.
Answered by: Technical Support
Date published: 2019-01-26

Can Clathrin LCB (G-7): sc-376414 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, Clathrin LCB (G-7): sc-376414 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from bonne fonctionnalitésj'ai testé cet anticorps en immunofluorescence, il fonctionne parfaitement
Date published: 2018-02-19
Rated 5 out of 5 by from Great!The sample antibody stained specficly in HeLa and MDCK cells!
Date published: 2017-11-27
Rated 4 out of 5 by from Robust band seen in WB using Hep G2 wholeRobust band seen in WB using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-06-08
Rated 4 out of 5 by from Strong band seen in WB using HeLa whole cellStrong band seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-03
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Clathrin Light Chain B/CLTB 抗体 (G-7) is rated 4.4 out of 5 by 5.
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