Date published: 2025-9-7
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Clathrin Light Chain Antibody (CON.1) - Western Blotting - Image 363923
Clathrin LC 抗体 (CON. 1): sc-12735. C6 (A) 和 A-10 (B) 全细胞裂解液中 Clathrin LC 表达的 Western 印迹分析.
Clathrin LC 抗体 (CON. 1): sc-12735. C6 (A) 和 A-10 (B) 全细胞裂解液中 Clathrin LC 表达的 Western 印迹分析.

Clathrin Light Chain 抗体 (CON.1): sc-12735

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寫評論提問

说明书
  • Clathrin Light Chain 抗体 CON.1 是小鼠单克隆 IgG2b κ，Clathrin Light Chain抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫bovine物种的clathrin light chain的氨基酸23-44
  • Clathrin Light Chain 抗体 (CON.1) 推荐用于WB, IP 和 IF，检测broad species 来源的 Clathrin LCA and LCB
  • 抗Clathrin Light Chain抗体（CON.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Clathrin Light Chain (CON.1): sc-12735的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Clathrin Light Chain Antibody (CON.1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Clathrin Light Chain Antibody (CON.1) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Clathrin抗体，包括Clathrin LC

Clathrin LC 抗体 (CON.1) 是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测多种物种中的Clathrin LC蛋白。抗Clathrin LC抗体（CON.1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®选项。Clathrin在细胞内运输中发挥着至关重要的作用，特别是在受体介导的内吞作用中，它有助于受体蛋白吞入包被有Clathrin的囊泡中。Clathrin LC蛋白主要存在于细胞质中，可组装成三叶形结构，由三个重链和三个轻链组成。这种结构排列有助于形成包被的囊泡，从而在细胞内运输蛋白质和脂质。轻链，特别是Clathrin轻链A（LCA）和Clathrin轻链B（LCB），负责调节三叶形结构在膜上的自组装，从而影响囊泡的形成效率。LCA和LCB由不同的基因编码，但具有60%的同源性，表现出相似的功能特性，包括组织特异性同工型的可替代性mRNA剪接。脑特异性轻链同工型包含插入序列，形成分子量更高的变体，对神经元功能和信号传导至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Clathrin Light Chain 抗体 (CON.1) 参考文献:

  1. 神经元特异性表达高分子量凝集素轻链  |  Wong, DH., et al. 1990. J Neurosci. 10: 3025-31. PMID: 2204686
  2. clathrin轻链LCb的优势与调节分泌途径的存在相关。  |  Acton, SL. and Brodsky, FM. 1990. J Cell Biol. 111: 1419-26. PMID: 2211818
  3. 包膜囊泡的结构和组装。  |  Pearse, BM. and Crowther, RA. 1987. Annu Rev Biophys Biophys Chem. 16: 49-68. PMID: 2885011
  4. Clathrin 和包被囊泡。  |  Pearse, BM. 1987. EMBO J. 6: 2507-12. PMID: 2890519
  5. 人类凝集素轻链的结构。三种哺乳动物轻链多态性的一致性。  |  Jackson, AP. and Parham, P. 1988. J Biol Chem. 263: 16688-95. PMID: 3267234
  6. 针对癌症的 Clathrin 轻链结合药物输送。  |  Jung, S., et al. 2023. Bioeng Transl Med. 8: e10273. PMID: 36684105
  7. 富含A型包涵体蛋白轻链的小胞外囊泡重塑微血管微环境，诱发肝细胞癌转移。  |  Xu, Y., et al. 2023. J Extracell Vesicles. 12: e12359. PMID: 37606345
  8. CLC2（Clathrin Light Chain 2）-ATG8h/ATG8i相互作用连接了由Clathrin介导的内吞作用（CME）和自噬通路。  |  Lan, H., et al. 2025. Autophagy. 21: 246-248. PMID: 39396122
  9. 人类凝集素轻链基因（CLTA 和 CLTB）的染色体位置和一些结构特征。  |  Ponnambalam, S., et al. 1994. Genomics. 24: 440-4. PMID: 7713494
  10. 钙调蛋白与凝集素包被囊泡, 三叉小体和轻链的相互作用。结合位点的定域。  |  Pley, UM., et al. 1995. J Biol Chem. 270: 2395-402. PMID: 7836475
  11. 凝集素轻链在阿尔茨海默病病理学中的参与。  |  Nakamura, Y., et al. 1994. Acta Neuropathol. 87: 23-31. PMID: 8140893
  12. 通过转染和基因干扰改变 clathrin 轻链的表达。  |  Acton, SL., et al. 1993. Mol Biol Cell. 4: 647-60. PMID: 8374173

订购信息

产品名称产品编号规格价格数量收藏夹

Clathrin Light Chain 抗体 (CON.1)

sc-12735
200 µg/ml
RMB2377.00

Clathrin Light Chain (CON.1): m-IgGκ BP-HRP 套装

sc-520584
200 µg Ab, 40 µg BP
RMB2662.00

Clathrin Light Chain (CON.1): m-IgG2b BP-HRP 套装

sc-548839
200 µg Ab; 10 µg BP
RMB2662.00

Clathrin Light Chain 抗体 (CON.1) AC

sc-12735 AC
500 µg/ml, 25% agarose
RMB3129.00

Clathrin Light Chain 抗体 (CON.1) HRP

sc-12735 HRP
200 µg/ml
RMB2377.00

Clathrin Light Chain 抗体 (CON.1) FITC

sc-12735 FITC
200 µg/ml
RMB2482.00

Clathrin Light Chain 抗体 (CON.1) PE

sc-12735 PE
200 µg/ml
RMB2580.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 488

sc-12735 AF488
200 µg/ml
RMB2685.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 546

sc-12735 AF546
200 µg/ml
RMB2685.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 594

sc-12735 AF594
200 µg/ml
RMB2685.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 647

sc-12735 AF647
200 µg/ml
RMB2685.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 680

sc-12735 AF680
200 µg/ml
RMB2685.00

Clathrin Light Chain 抗体 (CON.1) Alexa Fluor® 790

sc-12735 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with Clathrin LC (CON.1): sc-12735 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from good antibodyThe antibody was used in triple negative breast cancer cell lines and in colon cancer cell lines at the concentration of 1:500 diluted in TBS 0,1% Tween-20 and used overnight. It produce a specific band.
Date published: 2021-10-04
Rated 4 out of 5 by from good resultsI got good IF images in MDCK and HeLa cells with the sample antibody from Santa except for the slightly nonselective staining in cell nuclei.
Date published: 2017-11-27
Rated 1 out of 5 by from No signal in WBUsed at a 1:500 dilution on 35ug of HeLa lysate and did not get a signal after 30min exposure
Date published: 2016-12-06
Rated 4 out of 5 by from Used for studying adult stem cell/pluripotencyUsed for studying adult stem cell/pluripotency
Date published: 2015-04-29
Rated 4 out of 5 by from Publishable data in IF using rat PC12 cellsPublishable data in IF using rat PC12 cells -SCBT Publication Review
Date published: 2015-04-26
Rated 4 out of 5 by from Publishable data in WB using cattle brainPublishable data in WB using cattle brain. -SCBT Publication Review
Date published: 2015-04-02
Rated 4 out of 5 by from Nice band seen in WB using C6 whole cell lysateNice band seen in WB using C6 whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
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Clathrin Light Chain 抗体 (CON.1) is rated 3.8 out of 5 by 8.
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