Date published: 2025-9-7
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Chk2 Antibody (DCS-270) - Western Blotting - Image 354015
Chk2 抗体 (DCS-270): sc-56296. CCRF-CEM (A), K-562 (B), Jurkat (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.
Chk2 抗体 (DCS-270): sc-56296. CCRF-CEM (A), K-562 (B), Jurkat (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.

Chk2 抗体 (DCS-270): sc-56296

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寫評論提問

说明书
  • Chk2抗体(DCS-270)是小鼠单克隆IgG2a κ, 在14篇文献中引用，规格为200 µg/ml
  • 针对Chk2融合蛋白的抗体，来源于human
  • 推荐用于 human 来源的Chk2 WB, IP, IF 和 IHC(P)检测
  • 看 Chk2 (A-11): sc-17747，可查看有标记的 Chk2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Chk2 抗体 (DCS-270)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 Chk 抗体 包括 Chk2

细胞周期事件受到细胞周期蛋白依赖性激酶（Cdks）的顺序激活和失活以及细胞周期蛋白的水解调节，而Chk1和Chk2作为Cdks的调节因子参与这些过程。特别地，Chk1和Chk2在G2 DNA损伤检查点中发挥着至关重要的作用，它们通过响应DNA损伤来磷酸化Cdc25C。这种磷酸化作用抑制了Cdc25C的活性，从而阻止了细胞进入有丝分裂阶段。此外，Cdc25A、Cdc25B和Cdc25C蛋白酪氨酸磷酸酶则通过去磷酸化调节性酪氨酸残基上的Cdc2 p34来发挥有丝分裂激活剂的作用。值得一提的是，研究还表明Chk1可以在体外磷酸化Wee1，这为进一步证实细胞内由于Chk1过度表达而导致的Wee1超磷酸化状态提供了证据，这种状态是由Chk1对Wee1的磷酸化作用所引起的。

仅限研究使用。不适用于诊断和治疗用途。

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Chk2 抗体 (DCS-270)

sc-56296
200 µg/ml
RMB2377.00

I am using Chk2 (DCS-270): sc-56296 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great monoclonal antibodyNice robust IHCP stain and strong specific band detected by WB with little to no background
Date published: 2017-02-06
Rated 5 out of 5 by from Strong pulldown in IP in human UStrong pulldown in IP in human U-2-OS and transformed ATM-deficient cells. -SCBT Publication Review
Date published: 2015-05-02
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Good publishable data in WB in human HCT116Good publishable data in WB in human HCT116, TO53, HCC1937. -SCBT Publication Review
Date published: 2015-01-18
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Publishable Western blot results using 1:800Publishable Western blot results using 1:800. -SCBT Publication Review
Date published: 2014-06-29
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Chk2 抗体 (DCS-270) is rated 5.0 out of 5 by 6.
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