Date published: 2025-9-7
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Cdk5 Antibody (J-3) - Western Blotting - Image 7235
Cdk5 抗体 (J-3): sc-6247. NIH/3T3 (A), RAW 264. 7 (B), MM-142 (C) 和 WEHI-231 (D) 核提取物中 Cdk5 表达的 Western 印迹分析.
Cdk5 抗体 (J-3): sc-6247. NIH/3T3 (A), RAW 264. 7 (B), MM-142 (C) 和 WEHI-231 (D) 核提取物中 Cdk5 表达的 Western 印迹分析.

Cdk5 抗体 (J-3): sc-6247

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寫評論提問

说明书
  • Cdk5 抗体 J-3 是小鼠单克隆 IgG2b κ，Cdk5抗体, 在130篇文献中引用，规格为200 µg/ml
  • 免疫1-291氨基酸，对应于human来源Cdk5全长
  • Cdk5 抗体 (J-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Cdk5
  • 抗Cdk5抗体（J-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdk5 (J-3): sc-6247的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Cdk5 Antibody (J-3) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Cdk5 Antibody (J-3) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Cdk抗体，包括Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

Cdk5抗体（J-3）是一种小鼠单克隆IgG2b kappa轻链抗体，靶向细胞周期依赖性激酶5（Cdk5），也称为PSSALRE，是一种参与调节细胞周期和神经元功能的关键蛋白。Cdk5单克隆抗体（J-3）针对全长人类Cdk5蛋白的氨基酸1-291，对小鼠、大鼠和人类Cdk5蛋白均具有反应性，因此Cdk5（J-3）抗体非常适合用于蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附等实验。Cdk5在细胞周期的G1-S转换中起着关键作用，主要在成熟神经元中表达，在神经元分化过程中活性显著增加。Cdk5对于神经丝和tau蛋白等关键底物的磷酸化至关重要，而神经丝和tau蛋白对于维持神经元的结构和功能至关重要。通过调节这些过程，Cdk5对于正常的细胞运作和神经疾病的发展都是不可或缺的。Cdk5 单克隆抗体（J-3）有多种形式，包括非结合型和多种结合型，如琼脂糖、HRP、PE、FITC 和几种 Alexa Fluor® 结合物，为研究人员提供了满足实验需求的灵活选择。Cdk5（J-3）抗体能够精确检测和研究 Cdk5，有助于加深对 Cdk5 在细胞周期调节和神经生物学中作用的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdk5 抗体 (J-3) 参考文献:

  1. 新型 cdc2/CDC28 相关蛋白激酶基因家族的两个成员 PCTAIRE-1 和 PCTAIRE-3。  |  Okuda, T., et al. 1992. Oncogene. 7: 2249-58. PMID: 1437147
  2. 大鼠脑达能素 I 的体内磷酸化位点。  |  Graham, ME., et al. 2007. J Biol Chem. 282: 14695-707. PMID: 17376771
  3. CDK5：一种独特的CDK及其在神经系统中的多重作用。  |  Cortés, N., et al. 2019. J Alzheimers Dis. 68: 843-855. PMID: 30856110
  4. CDK5：细胞凋亡和细胞存活的关键调节因子。  |  Roufayel, R. and Murshid, N. 2019. Biomedicines. 7: PMID: 31698798
  5. CDK5：一个致癌基因还是一个抗致癌基因：位置、位置、位置。  |  Nikhil, K. and Shah, K. 2023. Mol Cancer. 22: 186. PMID: 37993880
  6. 细胞周期激酶  |  Pines, J. 1994. Semin Cancer Biol. 5: 305-13. PMID: 7803767
  7. 大鼠 Nb2 前 T 淋巴瘤细胞在催乳素诱导的 G1/S 转化过程中细胞周期蛋白 E, cdk2, cdk5 和 E2F-1 的协同基因表达。  |  Hosokawa, Y., et al. 1995. Biochem Mol Biol Int. 37: 393-9. PMID: 8673024
  8. 细胞周期蛋白, 细胞周期蛋白依赖性激酶和 cdk 抑制剂：对细胞周期控制和癌症的影响。  |  MacLachlan, TK., et al. 1995. Crit Rev Eukaryot Gene Expr. 5: 127-56. PMID: 8845581
  9. 细胞周期蛋白依赖性激酶 5 (CDK5) 在小鼠神经元中的胞内定位：CDK5 位于细胞核和细胞质中。  |  Ino, H. and Chiba, T. 1996. Brain Res. 732: 179-85. PMID: 8891282
  10. 大鼠小脑神经发生早期阶段 cdk5 的表达及与 p35nck5a 的关联；有丝分裂后神经元的酪氨酸去磷酸化和激活。  |  Lazaro, JB., et al. 1996. Neurosci Lett. 218: 21-4. PMID: 8939471
  11. 细胞周期蛋白依赖性激酶 4 和 6 抑制剂基因家族 p15, p16, p18 和 p19 在白血病和淋巴瘤中的作用。  |  Siebert, R., et al. 1996. Leuk Lymphoma. 23: 505-20. PMID: 9031081
  12. 神经肿瘤学的最新概念：细胞周期--综述。  |  Dirks, PB. and Rutka, JT. 1997. Neurosurgery. 40: 1000-13; discussion 1013-5. PMID: 9149259

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk5 抗体 (J-3)

sc-6247
200 µg/ml
RMB2377.00

Cdk5 (J-3): m-IgG Fc BP-HRP 套装

sc-528161
200 µg Ab; 10 µg BP
RMB2662.00

Cdk5 (J-3): m-IgGκ BP-HRP 套装

sc-520469
200 µg Ab, 40 µg BP
RMB2662.00

Cdk5 抗体 (J-3) AC

sc-6247 AC
500 µg/ml, 25% agarose
RMB3129.00

Cdk5 抗体 (J-3) HRP

sc-6247 HRP
200 µg/ml
RMB2377.00

Cdk5 抗体 (J-3) FITC

sc-6247 FITC
200 µg/ml
RMB2482.00

Cdk5 抗体 (J-3) PE

sc-6247 PE
200 µg/ml
RMB2580.00

Cdk5 抗体 (J-3) Alexa Fluor® 488

sc-6247 AF488
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) Alexa Fluor® 546

sc-6247 AF546
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) Alexa Fluor® 594

sc-6247 AF594
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) Alexa Fluor® 647

sc-6247 AF647
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) Alexa Fluor® 680

sc-6247 AF680
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) Alexa Fluor® 790

sc-6247 AF790
200 µg/ml
RMB2685.00

Cdk5 抗体 (J-3) TRITC

sc-6247 TRITC
200 µg/ml
RMB2407.00

Have you checked that this antibody is specific for CDK5 and does not recognize other related CDKs including CDK1 and CDK2? Thanks

Asked by: MRou
Thank you for your question. Our mouse monoclonal Cdk5 Antibody (J-3): sc-6247 has been experimentally validated for specificity in Cdk5 CRISPR/Cas9 KO transfected HEK293T whole cell lysates. This data is available on our website. Transfections were performed using our Cdk5 CRISPR/Cas9 KO Plasmid: sc-400161 plus Cdk5 HDR Plasmid: sc-400161-HDR.
Answered by: Technical Support
Date published: 2020-05-08

Can Cdk5 Antibody (J-3): sc-6247 be used for detection of Cdk5 of monkey origin?

Asked by: jenni
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from works perfectlyGot this ab as a free sample, works very fine. Clear band at the right molecular weight in mouse brain lysates and SH cells, highly recommended.
Date published: 2021-02-03
Rated 5 out of 5 by from good antibodygood antibody, correct band size by western blotting.
Date published: 2017-11-22
Rated 5 out of 5 by from Detects endogenous proteinWorks well enough for western blot, for endogenous levels of cdk5 in human cell lines and mouse neurons. Used at 1:500.
Date published: 2017-11-21
Rated 5 out of 5 by from Clear and powerful signalUsing antibody 1:1000 working in WB and HeLa cells, we used this antibody for many years and always been good signals.
Date published: 2017-11-02
Rated 5 out of 5 by from good signalThe antibody can be used for the detection of Cdk5 and provide a good signal, which can help us to study the experime
Date published: 2017-10-20
Rated 5 out of 5 by from Really greatWe got obtained specific band is very clear and powerful signal with Cdk5 Antibody. Knowledge of the sensitivity and specificity of the antibody leads to their more rational selection.
Date published: 2017-10-18
Rated 5 out of 5 by from great featurework well in WB at 1:100,and the strip is very clear.
Date published: 2017-10-08
Rated 5 out of 5 by from GreatThis antibody works for mouse brain tissue western blot samples
Date published: 2017-09-06
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Cdk5 抗体 (J-3) is rated 5.0 out of 5 by 27.
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