Date published: 2025-9-7
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Cdc7 Antibody (SPM171) - Western Blotting - Image 15412
Cdc7 抗体 (SPM171): sc-56275. HeLa (A), ECV304 (B) 和 Y79 (C) 全细胞裂解液中 Cdc7 表达的 Western 印迹分析.
Cdc7 抗体 (SPM171): sc-56275. HeLa (A), ECV304 (B) 和 Y79 (C) 全细胞裂解液中 Cdc7 表达的 Western 印迹分析.

Cdc7 抗体 (SPM171): sc-56275

4.6(7)
寫評論提問

说明书
  • Cdc7 抗体 SPM171 是小鼠单克隆 IgG1 κ，Cdc7抗体, 在10篇文献中引用，规格为200 µg/ml
  • 针对全长Cdc7，human起源
  • 推荐用于 human 来源的Cdc7 WB, IP, IF 和 IHC(P)检测
  • 关于如何获取Cdc7 (SPM171): sc-56275的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Cdc7 抗体 (SPM171) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc7 抗体 (SPM171) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc7 抗体 (SPM171) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组化 (IHCP) 检测人源 Cdc7 蛋白。Cdc7 蛋白在 DNA 复制调控中发挥着关键作用，特别是在细胞周期的 S 期激活复制起源。Cdc7 与调节亚基 Dbf4 形成一个重要的复合物，后者对激酶活性至关重要。该复合物负责启动 DNA 复制所需的几种关键蛋白的磷酸化，包括六个迷你染色体维持（Mcm）蛋白中的五个以及 DNA 聚合酶 α-primase的 p180亚基。Cdc7和Dbf4的精确调控至关重要，因为Dbf4的水平在整个细胞周期中波动，在G1期不存在，而在S期和G2期积累。在染色体分离开始时，无性繁殖促进复合体会及时降解 Dbf4，以确保复制前复合体不会立即重新启动，从而保持基因组的稳定性。Cdc7 在整个细胞周期中的持续表达突显了它在 DNA 复制和细胞分裂中的重要性，这使得 Cdc7 (SPM171) 抗体成为研究细胞周期调控和相关过程的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc7 抗体 (SPM171) 参考文献:

  1. Cdc7 激酶调节亚基 Dbf4p 在形成起始适配复制起源中的作用  |  Pasero, P., et al. 1999. Genes Dev. 13: 2159-76. PMID: 10465792
  2. Cdc7p-Dbf4p 激酶在 S 期与染色质结合，并受 APC 和 RAD53 检查点途径的调控。  |  Weinreich, M. and Stillman, B. 1999. EMBO J. 18: 5334-46. PMID: 10508166
  3. 人类和鼠类 DBF4 同源基因的鉴定, 特征描述和染色体定位。  |  Lepke, M., et al. 1999. Mol Gen Genet. 262: 220-9. PMID: 10517317
  4. 哺乳动物 Cdc7-Dbf4 蛋白激酶复合物对 DNA 复制的启动至关重要。  |  Jiang, W., et al. 1999. EMBO J. 18: 5703-13. PMID: 10523313
  5. Dbf4p是一种重要的S期促进因子，是无相促进复合体降解的靶标。  |  Ferreira, MF., et al. 2000. Mol Cell Biol. 20: 242-8. PMID: 10594027
  6. Cdc7 激酶复合体：DNA 复制启动过程中的关键调节因子。  |  Masai, H. and Arai, K. 2002. J Cell Physiol. 190: 287-96. PMID: 11857444
  7. Cdc7 激酶对 MCM4 的磷酸化促进了它与染色质上的 Cdc45 的相互作用。  |  Masai, H., et al. 2006. J Biol Chem. 281: 39249-61. PMID: 17046832
  8. Cdc7 是一种活跃的激酶，可用于承受复制压力的人类癌细胞。  |  Tenca, P., et al. 2007. J Biol Chem. 282: 208-15. PMID: 17062569
  9. 靶向蛋白降解揭示了 CDC7 不依赖于 G1/S 过渡。  |  Suski, JM., et al. 2022. Nature. 605: 357-365. PMID: 35508654
  10. CDC7 抑制会诱导复制应激介导的非整倍体细胞，这种细胞具有炎症表型，使肿瘤对免疫检查点阻断剂敏感。  |  Morita, TY., et al. 2023. Nat Commun. 14: 7490. PMID: 37980406
  11. CDC7 抑制通过 MYC 降解损害肺癌和前列腺肿瘤的神经内分泌转化体。  |  Quintanal-Villalonga, A., et al. 2024. Signal Transduct Target Ther. 9: 189. PMID: 39054323
  12. S 期的起源点火需要 Cdc7 蛋白激酶。  |  Bousset, K. and Diffley, JF. 1998. Genes Dev. 12: 480-90. PMID: 9472017

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc7 抗体 (SPM171)

sc-56275
200 µg/ml
$316.00

Cdc7 (SPM171): m-IgG Fc BP-HRP 套装

sc-528615
200 µg Ab; 10 µg BP
$354.00

Cdc7 (SPM171): m-IgGκ BP-HRP 套装

sc-521036
200 µg Ab, 40 µg BP
$354.00

Cdc7 (SPM171): m-IgG1 BP-HRP 套装

sc-543030
200 µg Ab; 20 µg BP
$354.00

What is the recommended starting dilution for Western blot for sc-56275: Cdc7 (SPM171) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

How much is the volume of sc-56275? How many staining of specimen with moderate size is possible? Dilution has been mentioned in datasheet.(200 ug / ml)

Asked by: jaafariz
Thank you very much for your inquiry. The Cdc7 Antibody (SPM171): sc-56275 is provided at 200 µg in 1 ml buffer. The volume you will receive is 1 ml. We would recommend to use a starting dilution of 1:50 for IHC(P). The number of slides that you can stain with one vial depends on the volume you are using for incubating each slide. Please find further information in our protocols under the following link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support Europe
Date published: 2017-02-28
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Rated 5 out of 5 by from Clean bandsI requested a sample of this antibody and tested it four cell lines (DLD1, HeLa, RPE1 and U2OS) in different fractions (Whole cell, soluble and chromatin) and it detects a band of approximately 60 kDa.
Date published: 2019-07-03
Rated 5 out of 5 by from Good antibodyStrong antibody for WB (1:100) dilution) tested in HCT116 whole cell lysates.
Date published: 2018-02-19
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 4 out of 5 by from Good for WBVery strong and specific band for western blot in human cell samples
Date published: 2017-10-12
Rated 4 out of 5 by from Performs well in nonPerforms well in non-transfected 293T, human Cdc7 transfected 293T and HeLa whole cell lysates by WB. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Good specificity and single strength forGood specificity and single strength for western blot analysis of Cdc7 expression in non-transfected and human Cdc7 transfected 293T whole cell lysates. - SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Strong signal with a bit of background forStrong signal with a bit of background for WB in HeLa, ECV304 and Y79 whole cell lysates. - SCBT QC
Date published: 2014-05-09
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Cdc7 抗体 (SPM171) is rated 4.6 out of 5 by 7.
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