Date published: 2025-9-7
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Cdc25A Antibody (F-6) - Western Blotting - Image 353936
Cdc25A 抗体 (F-6): sc-7389. SK-N-MC (A), A549 (B), Hep G2 (C), MCF7 (D), A-431 (E) 和 HL-60 (F) 全细胞裂解液中 Cdc25A 表达的 Western 印迹分析.
Cdc25A 抗体 (F-6): sc-7389. SK-N-MC (A), A549 (B), Hep G2 (C), MCF7 (D), A-431 (E) 和 HL-60 (F) 全细胞裂解液中 Cdc25A 表达的 Western 印迹分析.

Cdc25A 抗体 (F-6): sc-7389

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寫評論提問

说明书
  • Cdc25A 抗体 F-6 是小鼠单克隆 IgG1 κ，Cdc25A抗体, 在245篇文献中引用，规格为200 µg/ml
  • 抗原表位位于mouse物种的Cdc25A的C-terminus
  • Cdc25A 抗体 (F-6) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 Cdc25A
  • 抗Cdc25A抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdc25A (F-6): sc-7389的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是Cdc25A Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Cdc25A Antibody (F-6) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Cdc抗体，包括Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26, Cdc27, Cdc28, Cdc34, Cdc37, Cdc4, Cdc40和Cdc42

Cdc25A抗体（F-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人类来源的Cdc25A。Cdc25A单克隆抗体（F-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Cdc25A在细胞周期调节中发挥着关键作用，特别是在从G2期到有丝分裂的过渡中。Cdc25A是一种磷酸酶，可去磷酸化细胞周期蛋白依赖性激酶Cdc2，从而激活Cdc2/细胞周期蛋白B复合物，这对有丝分裂的启动至关重要。对Cdc25A的精确调控至关重要，因为过量的Cdc25A会导致细胞分裂失控，并可能引发多种癌症。Cdc25A的活性受到翻译后修饰的严格控制，包括磷酸化，磷酸化可以激活或抑制功能，这凸显了理解细胞周期进展和肿瘤发生中的调控机制的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc25A 抗体 (F-6) 参考文献:

  1. cdc25 蛋白作用的线索。  |  Moreno, S. and Nurse, P. 1991. Nature. 351: 194. PMID: 1645849
  2. 裂殖酵母中细胞周期蛋白-cdc2的核区和纺锤极体区不同。  |  Alfa, CE., et al. 1990. Nature. 347: 680-2. PMID: 1699136
  3. B 型细胞周期蛋白对 cdc25 酪氨酸磷酸酶的特异性激活：有丝分裂细胞周期蛋白多种作用的证据。  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
  4. cdc25 是一种特异性酪氨酸磷酸酶，可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  5. 成熟促进因子对 M 期的控制  |  Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
  6. 用磷酸酶直接激活 cdc2：识别 p13suc1 敏感和不敏感步骤。  |  Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
  7. 裂殖酵母中有丝分裂诱导子 p80cdc25 周期性积累对有丝分裂的调控。  |  Moreno, S., et al. 1990. Nature. 344: 549-52. PMID: 2320127
  8. 裂殖酵母 cdc2+ 蛋白激酶的酪氨酸磷酸化调节有丝分裂的进入。  |  Gould, KL. and Nurse, P. 1989. Nature. 342: 39-45. PMID: 2682257
  9. 多米诺骨牌与时钟：细胞周期两种观点的结合。  |  Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
  10. Cdc25A通过PKM2去磷酸化上调ErbB2，从而抑制宫颈癌细胞中自噬介导的铁死亡。  |  Wang, C., et al. 2021. Cell Death Dis. 12: 1055. PMID: 34743185
  11. SRSF10：通过触发外显子6跳变来稳定CDC25A，促进肝癌的发生。  |  Liu, X., et al. 2022. J Exp Clin Cancer Res. 41: 353. PMID: 36539837
  12. CHK1-CDC25A-CDK1调控小鼠早期胚胎的细胞周期进展并保护基因组完整性。  |  Knoblochova, L., et al. 2023. EMBO Rep. 24: e56530. PMID: 37694680

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc25A 抗体 (F-6)

sc-7389
200 µg/ml
RMB2377.00

Cdc25A (F-6): m-IgG1 BP-HRP 套装

sc-531629
200 µg Ab; 20 µg BP
RMB2662.00

Cdc25A 抗体 (F-6) AC

sc-7389 AC
500 µg/ml, 25% agarose
RMB3129.00

Cdc25A 抗体 (F-6) HRP

sc-7389 HRP
200 µg/ml
RMB2377.00

Cdc25A 抗体 (F-6) FITC

sc-7389 FITC
200 µg/ml
RMB2482.00

Cdc25A 抗体 (F-6) PE

sc-7389 PE
200 µg/ml
RMB2580.00

Cdc25A 抗体 (F-6) Alexa Fluor® 488

sc-7389 AF488
200 µg/ml
RMB2685.00

Cdc25A 抗体 (F-6) Alexa Fluor® 546

sc-7389 AF546
200 µg/ml
RMB2685.00

Cdc25A 抗体 (F-6) Alexa Fluor® 594

sc-7389 AF594
200 µg/ml
RMB2685.00

Cdc25A 抗体 (F-6) Alexa Fluor® 647

sc-7389 AF647
200 µg/ml
RMB2685.00

Cdc25A 抗体 (F-6) Alexa Fluor® 680

sc-7389 AF680
200 µg/ml
RMB2685.00

Cdc25A 抗体 (F-6) Alexa Fluor® 790

sc-7389 AF790
200 µg/ml
RMB2685.00

How can I get the antigen peptide for Competition Assay to verify the specific bands in my samples?

Asked by: Stav
This antibody is not raised against a peptide, but rather is raised against a recombinant protein. Therefore, no blocking peptide antigen is available. We would recommend using alternative positive and negative controls to verify the specificity of your results with this antibody.
Answered by: Technical Support
Date published: 2019-04-13

I bought this antibody after seeing several citations, however it does not show any bands at 1:200 dilution as suggested by other users. I used U2OS cell lysate known to express CDC25A from many publications. Please help me !

Asked by: emily and jack
Thank you for your question. Please contact our Technical Service Department and we can assist you in troubleshooting the antibody, Cdc25A (F-6): sc-7389. A dilution of 1:200 should be appropriate, and we recommend loading 50 µg of whole cell lysate protein. A link to our complete recommended protocol for Western blotting is below as a reference. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-11-18

I recently brought Cdc25a antibody from Santa Cruz (sc-7389) , after seeing several citations. But I am not getting any expression in HEK293T cells, while several articles showed Cdc25A expression in HEK293T cells. Please suggest me some tips?

Asked by: Das S
We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat, or reach out to your local distributor. We are happy to help you troubleshoot the antibody!
Answered by: Tech Service
Date published: 2017-06-21

What is the recommended secondary when using this antibody for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-23
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Rated 4 out of 5 by from Good antibody!This antibody gives good result by western blot! I used it in 1:200 dilution in PBS 0,02% tween (o.n night incubation at 4°) and I obtained a clear and specific band at the proper molecular weight in Raji cell line.
Date published: 2022-01-27
Rated 3 out of 5 by from CDC25A in 1-cell stage embryosCDC25A was not detected in 1-cell stage embryos 1 hour after 10 Gy irradiation.
Date published: 2021-11-08
Rated 3 out of 5 by from anti-CDC25AGood monoclonal antibody for the detection of endogenous CDC25A by Western blot. Recommended dilution: 1/100
Date published: 2019-02-11
Rated 3 out of 5 by from Great to detect degradation after damageWe've been using this for quite long time. It worked nicely to detect Cdc25A degradation, especially after DNA damage. It worked better for human, for mouse it is much weaker.
Date published: 2018-01-26
Rated 4 out of 5 by from It works wellThis is a free sample, and I try it in Hela cells, it works well, but may need high dilution (1:100).
Date published: 2018-01-08
Rated 5 out of 5 by from Great features!I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 2 out of 5 by from The function is not goodIt works in western blot at 1:100,gastric cancers. but we cannot get a clear band.
Date published: 2017-09-21
Rated 5 out of 5 by from GREAT FEATURESI can get clear bands when using protein samples from T24 and 5637 in WB.
Date published: 2017-09-08
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Cdc25A 抗体 (F-6) is rated 4.1 out of 5 by 18.
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