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Cdc20 Antibody (AR12) - Western Blotting - Image 12902
Cdc20 抗体 (AR12): sc-53398. HeLa (A), Nocodazole 处理的 HeLa (B) 和 Ramos (C) 全细胞裂解液中 Cdc20 表达的 Western 印迹分析.
Cdc20 抗体 (AR12): sc-53398. HeLa (A), Nocodazole 处理的 HeLa (B) 和 Ramos (C) 全细胞裂解液中 Cdc20 表达的 Western 印迹分析.

Cdc20 抗体 (AR12): sc-53398

4.2(5)
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说明书
  • Cdc20抗体(AR12)是小鼠单克隆IgG1 κ, 在1篇文献中引用，规格为200 µg/ml
  • 针对尿素变性His6 Cdc20重组蛋白的抗体，源自human
  • 推荐用于 mouse, rat 和 human 来源的Cdc20 WB, IP, IF 和 IHC(P)检测
  • 看 Cdc20 (E-7): sc-13162，可查看有标记的 Cdc20 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Cdc20 抗体 (AR12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc20 抗体 (AR12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

细胞周期蛋白是与激酶结合的调节亚单位，控制着细胞周期进程中的许多重要步骤。Cdc2蛋白激酶（p34Cdc2）在体外表现出蛋白激酶活性，并与细胞周期蛋白B和与p13suc1同源的蛋白质存在于复合物中。Cdc2激酶是M期促进因子（MPF）的活性亚单位和M期特异性组蛋白H1激酶。p34Cdc2/细胞周期蛋白B复合物是G2到M期过渡所必需的。另一种称为Cdc20的细胞周期依赖性蛋白激酶与酿酒酵母蛋白Cdc20和Cdc4具有高度同源性。Cdc20转录本在生长阶段的各种培养细胞系中易于检测，但在细胞生长被化学抑制时消失。Cdc20对α-酪蛋白和髓鞘碱性蛋白表现出激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc20 抗体 (AR12)

sc-53398
200 µg/ml
RMB2377.00

Cdc20 (AR12): m-IgG Fc BP-HRP 套装

sc-536954
200 µg Ab; 10 µg BP
RMB2662.00

Cdc20 (AR12): m-IgGκ BP-HRP 套装

sc-534131
200 µg Ab; 40 µg BP
RMB2662.00

Cdc20 (AR12): m-IgG1 BP-HRP 套装

sc-544920
200 µg Ab; 20 µg BP
RMB2662.00

Can p55 CDC (AR12): sc-53398 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, p55 CDC (AR12): sc-53398 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Decent WB signal with some background achievedDecent WB signal with some background achieved using HeLa, Nocodazole treated HeLa and Ramos whole cell lysates. -SCBT QC
Date published: 2015-06-25
Rated 4 out of 5 by from Clear immunoperoxidase cytoplasmic stainingClear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with U87MG cells and U373MG. -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Very favorable WB achieved using 293T wholeVery favorable WB achieved using 293T whole cell lysate. -SCBT QC
Date published: 2015-01-19
Rated 4 out of 5 by from WB publishable data with human U87MG cellsWB publishable data with human U87MG cells and U373MG - SCBT Publication Review
Date published: 2015-01-04
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Cdc20抗体 (AR12) is rated 4.2 out of 5 by 5.
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