Date published: 2025-9-8

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Cdc20 抗体 (E-7): sc-13162

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说明书
  • Cdc20 抗体 E-7 是小鼠单克隆 IgG1 κ,Cdc20抗体, 在115篇文献中引用,规格为200 µg/ml
  • 免疫human物种的p55 CDC的氨基酸1-175
  • Cdc20 抗体 (E-7) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Cdc20
  • 抗Cdc20抗体(E-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdc20 (E-7): sc-13162免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Cdc20 抗体 (E-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc20 抗体 (E-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Cdc20抗体(E-7)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学(IHC)检测小鼠、大鼠和人类来源的Cdc20。Cdc20单克隆抗体(E-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Cdc20在细胞周期调节中起着至关重要的作用,特别是在从G2期到有丝分裂的过渡中,它通过激活对细胞周期蛋白和其他调节蛋白的降解至关重要的后端促进复合物/环状体(APC/C)来发挥作用。这种降解对于细胞周期的正常进行至关重要,可确保细胞在正确的时间准确分裂。Cdc20失调会导致细胞分裂异常,与多种癌症有关,因此Cdc20成为细胞周期动力学和癌症生物学研究的重要目标。在生长阶段,Cdc20转录物很容易在各种培养细胞系中检测到,但当细胞生长被化学抑制时,其表达会减少,这凸显了Cdc20在细胞增殖中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc20 抗体 (E-7) 参考文献:

  1. 人类 p55(CDC)/Cdc20 与细胞周期蛋白 A 结合,并被细胞周期蛋白 A-Cdk2 复合物磷酸化。  |  Ohtoshi, A., et al. 2000. Biochem Biophys Res Commun. 268: 530-4. PMID: 10679238
  2. MPF 的摆动伴随着 cdc25 和 cdc2-cyclin B 之间的周期性结合。  |  Jessus, C. and Beach, D. 1992. Cell. 68: 323-32. PMID: 1310257
  3. 可塑性相关转录因子 zif268 对神经元 cdc20 (p55cdc) 表达的调控。  |  Conway, AM., et al. 2007. Synapse. 61: 463-8. PMID: 17372985
  4. cdc2 的可逆酪氨酸磷酸化:进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  5. 人细胞周期蛋白 cDNA 的分离:细胞周期蛋白 mRNA 和蛋白质在细胞周期中的调控以及与 p34cdc2 相互作用的证据。  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
  6. cdc2 是 M 期特异性组蛋白 H1 激酶的一个组成部分:与 MPF 相同的证据。  |  Arion, D., et al. 1988. Cell. 55: 371-8. PMID: 2844417
  7. 爪蟾 cdc2 蛋白是细胞质有丝分裂调节器 MPF 的一个组成部分。  |  Dunphy, WG., et al. 1988. Cell. 54: 423-31. PMID: 3293802
  8. p13suc1 在裂殖酵母细胞分裂周期中是 p34cdc2 蛋白激酶的一个组成部分。  |  Brizuela, L., et al. 1987. EMBO J. 6: 3507-14. PMID: 3322810
  9. CDC20在女性生殖系统癌症中的最新进展和前景。  |  Ni, K. and Hong, L. 2023. Curr Mol Med. 23: 193-199. PMID: 35319365
  10. EIF4A3/CDC20轴在子宫内膜癌中的致癌作用。  |  Lin, Y., et al. 2024. J Mol Med (Berl). 102: 1395-1410. PMID: 39316093
  11. 肿瘤抑制因子TPD52调控的内质网应激受APCCdc20调节。  |  Dan, W., et al. 2024. Adv Sci (Weinh). 11: e2405441. PMID: 39401430
  12. 存在于分裂细胞中的哺乳动物新蛋白 p55CDC 与蛋白激酶活性有关,与酿酒酵母细胞分裂周期蛋白 Cdc20 和 Cdc4 同源。  |  Weinstein, J., et al. 1994. Mol Cell Biol. 14: 3350-63. PMID: 7513050

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc20 抗体 (E-7)

sc-13162
200 µg/ml
RMB2377.00

Cdc20 (E-7): m-IgG Fc BP-HRP 套装

sc-528280
200 µg Ab; 10 µg BP
RMB2662.00

Cdc20 (E-7): m-IgGκ BP-HRP 套装

sc-520619
200 µg Ab, 40 µg BP
RMB2662.00

Cdc20 (E-7): m-IgG1 BP-HRP 套装

sc-542850
200 µg Ab; 20 µg BP
RMB2662.00

Cdc20 抗体 (E-7) AC

sc-13162 AC
500 µg/ml, 25% agarose
RMB3129.00

Cdc20 抗体 (E-7) HRP

sc-13162 HRP
200 µg/ml
RMB2377.00

Cdc20 抗体 (E-7) FITC

sc-13162 FITC
200 µg/ml
RMB2482.00

Cdc20 抗体 (E-7) PE

sc-13162 PE
200 µg/ml
RMB2580.00

Cdc20 抗体 (E-7) Alexa Fluor® 488

sc-13162 AF488
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) Alexa Fluor® 546

sc-13162 AF546
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) Alexa Fluor® 594

sc-13162 AF594
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) Alexa Fluor® 647

sc-13162 AF647
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) Alexa Fluor® 680

sc-13162 AF680
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) Alexa Fluor® 790

sc-13162 AF790
200 µg/ml
RMB2685.00

Cdc20 抗体 (E-7) TRITC

sc-13162 TRITC
200 µg/ml
RMB2407.00

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-13162 PE), fluorescein (sc-13162 FITC), Alexa Fluor® 488 (sc-13162 AF488) or Alexa Fluor® 647 (sc-13162 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 5 out of 5 by from Good for western blottingWorks well to detect CDC20 by western blotting in lysates from human MCF10A cells
Date published: 2023-03-22
Rated 4 out of 5 by from Good for western blotThe antibody can detect a specific band with 1:500 dilution for western blot. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 4 out of 5 by from Works well for WBworks wells for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Good for Western blotThis antibody worked well for western blot using whole cell lysate from human breast cancer cell lines.
Date published: 2017-11-17
Rated 5 out of 5 by from wb goodgood antibody ! dilution used is1:500,except one nonspecial band at 30KD
Date published: 2017-11-15
Rated 4 out of 5 by from not very goodThis antibody's specificity was not very good,and expect to improve。
Date published: 2017-09-12
Rated 5 out of 5 by from Great !the antibody works very well in IHC(P), 1:75 dilution is appropriate,brown particles can be clearly seen in the cytoplasm. No nonspecific staining.
Date published: 2017-04-12
Rated 5 out of 5 by from Great!The antibody is quite strong and specific although we could see a few bands near 55-kDa.
Date published: 2017-03-14
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Cdc20 抗体 (E-7) is rated 4.8 out of 5 by 21.
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